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Apelin Promotes Endothelial Progenitor Cell Angiogenesis in Rheumatoid Arthritis Disease via the miR-525-5p/Angiopoietin-1 Pathway

Angiogenesis is a critical process in the formation of new capillaries and a key participant in rheumatoid arthritis (RA) pathogenesis. The adipokine apelin (APLN) plays critical roles in several cellular functions, including angiogenesis. We report that APLN treatment of RA synovial fibroblasts (RA...

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Autores principales: Chang, Ting-Kuo, Zhong, You-Han, Liu, Shan-Chi, Huang, Chien-Chung, Tsai, Chun-Hao, Lee, Hsiang-Ping, Wang, Shih-Wei, Hsu, Chin-Jung, Tang, Chih-Hsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511637/
https://www.ncbi.nlm.nih.gov/pubmed/34659230
http://dx.doi.org/10.3389/fimmu.2021.737990
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author Chang, Ting-Kuo
Zhong, You-Han
Liu, Shan-Chi
Huang, Chien-Chung
Tsai, Chun-Hao
Lee, Hsiang-Ping
Wang, Shih-Wei
Hsu, Chin-Jung
Tang, Chih-Hsin
author_facet Chang, Ting-Kuo
Zhong, You-Han
Liu, Shan-Chi
Huang, Chien-Chung
Tsai, Chun-Hao
Lee, Hsiang-Ping
Wang, Shih-Wei
Hsu, Chin-Jung
Tang, Chih-Hsin
author_sort Chang, Ting-Kuo
collection PubMed
description Angiogenesis is a critical process in the formation of new capillaries and a key participant in rheumatoid arthritis (RA) pathogenesis. The adipokine apelin (APLN) plays critical roles in several cellular functions, including angiogenesis. We report that APLN treatment of RA synovial fibroblasts (RASFs) increased angiopoietin-1 (Ang1) expression. Ang1 antibody abolished endothelial progenitor cell (EPC) tube formation and migration in conditioned medium from APLN-treated RASFs. We also found significantly higher levels of APLN and Ang1 expression in synovial fluid from RA patients compared with those with osteoarthritis. APLN facilitated Ang1-dependent EPC angiogenesis by inhibiting miR-525-5p synthesis via phospholipase C gamma (PLCγ) and protein kinase C alpha (PKCα) signaling. Importantly, infection with APLN shRNA mitigated EPC angiogenesis, articular swelling, and cartilage erosion in ankle joints of mice with collagen-induced arthritis. APLN is therefore a novel therapeutic target for RA.
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spelling pubmed-85116372021-10-14 Apelin Promotes Endothelial Progenitor Cell Angiogenesis in Rheumatoid Arthritis Disease via the miR-525-5p/Angiopoietin-1 Pathway Chang, Ting-Kuo Zhong, You-Han Liu, Shan-Chi Huang, Chien-Chung Tsai, Chun-Hao Lee, Hsiang-Ping Wang, Shih-Wei Hsu, Chin-Jung Tang, Chih-Hsin Front Immunol Immunology Angiogenesis is a critical process in the formation of new capillaries and a key participant in rheumatoid arthritis (RA) pathogenesis. The adipokine apelin (APLN) plays critical roles in several cellular functions, including angiogenesis. We report that APLN treatment of RA synovial fibroblasts (RASFs) increased angiopoietin-1 (Ang1) expression. Ang1 antibody abolished endothelial progenitor cell (EPC) tube formation and migration in conditioned medium from APLN-treated RASFs. We also found significantly higher levels of APLN and Ang1 expression in synovial fluid from RA patients compared with those with osteoarthritis. APLN facilitated Ang1-dependent EPC angiogenesis by inhibiting miR-525-5p synthesis via phospholipase C gamma (PLCγ) and protein kinase C alpha (PKCα) signaling. Importantly, infection with APLN shRNA mitigated EPC angiogenesis, articular swelling, and cartilage erosion in ankle joints of mice with collagen-induced arthritis. APLN is therefore a novel therapeutic target for RA. Frontiers Media S.A. 2021-09-29 /pmc/articles/PMC8511637/ /pubmed/34659230 http://dx.doi.org/10.3389/fimmu.2021.737990 Text en Copyright © 2021 Chang, Zhong, Liu, Huang, Tsai, Lee, Wang, Hsu and Tang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Chang, Ting-Kuo
Zhong, You-Han
Liu, Shan-Chi
Huang, Chien-Chung
Tsai, Chun-Hao
Lee, Hsiang-Ping
Wang, Shih-Wei
Hsu, Chin-Jung
Tang, Chih-Hsin
Apelin Promotes Endothelial Progenitor Cell Angiogenesis in Rheumatoid Arthritis Disease via the miR-525-5p/Angiopoietin-1 Pathway
title Apelin Promotes Endothelial Progenitor Cell Angiogenesis in Rheumatoid Arthritis Disease via the miR-525-5p/Angiopoietin-1 Pathway
title_full Apelin Promotes Endothelial Progenitor Cell Angiogenesis in Rheumatoid Arthritis Disease via the miR-525-5p/Angiopoietin-1 Pathway
title_fullStr Apelin Promotes Endothelial Progenitor Cell Angiogenesis in Rheumatoid Arthritis Disease via the miR-525-5p/Angiopoietin-1 Pathway
title_full_unstemmed Apelin Promotes Endothelial Progenitor Cell Angiogenesis in Rheumatoid Arthritis Disease via the miR-525-5p/Angiopoietin-1 Pathway
title_short Apelin Promotes Endothelial Progenitor Cell Angiogenesis in Rheumatoid Arthritis Disease via the miR-525-5p/Angiopoietin-1 Pathway
title_sort apelin promotes endothelial progenitor cell angiogenesis in rheumatoid arthritis disease via the mir-525-5p/angiopoietin-1 pathway
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511637/
https://www.ncbi.nlm.nih.gov/pubmed/34659230
http://dx.doi.org/10.3389/fimmu.2021.737990
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