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Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites

Oil pollution is of increasing concern for environmental safety and the use of microbial surfactants in oil remediation has become inevitable for their efficacy and ecofriendly nature. In this work, biosurfactants of bacteria isolated from oil-contaminated soil have been characterized. Four potent b...

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Autores principales: Ali, Ferdausi, Das, Sharup, Hossain, Tanim Jabid, Chowdhury, Sumaiya Islam, Zedny, Subrina Akter, Das, Tuhin, Ahmed Chowdhury, Mohammad Nazmul, Uddin, Mohammad Seraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511774/
https://www.ncbi.nlm.nih.gov/pubmed/34659780
http://dx.doi.org/10.1098/rsos.211003
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author Ali, Ferdausi
Das, Sharup
Hossain, Tanim Jabid
Chowdhury, Sumaiya Islam
Zedny, Subrina Akter
Das, Tuhin
Ahmed Chowdhury, Mohammad Nazmul
Uddin, Mohammad Seraj
author_facet Ali, Ferdausi
Das, Sharup
Hossain, Tanim Jabid
Chowdhury, Sumaiya Islam
Zedny, Subrina Akter
Das, Tuhin
Ahmed Chowdhury, Mohammad Nazmul
Uddin, Mohammad Seraj
author_sort Ali, Ferdausi
collection PubMed
description Oil pollution is of increasing concern for environmental safety and the use of microbial surfactants in oil remediation has become inevitable for their efficacy and ecofriendly nature. In this work, biosurfactants of bacteria isolated from oil-contaminated soil have been characterized. Four potent biosurfactant-producing strains (SD4, SD11, SD12 and SD13) were selected from 27 isolates based on drop collapse assay and emulsification index, and identified as species belonging to Bacillus, Burkholderia, Providencia and Klebsiella, revealed from their 16S rRNA gene-based analysis. Detailed morphological and biochemical characteristics of each selected isolate were determined. Their growth conditions for maximum biosurfactant production were optimized and found quite similar among the four isolates with a pH of 3.0 and temperature 37°C after 6 or 7 days of growth on kerosene. The biosurfactants of SD4, SD11 and SD12 appeared to be glycolipids and that of SD13 a lipopeptide. Emulsification activity of most of the biosurfactants was stable at low and high temperatures (4–100°C), a wide range of pH (2–10) and salt concentrations (2–7% NaCl). Each biosurfactant showed antimicrobial activity against two or more pathogenic bacteria. The biosurfactants were well-capable of emulsifying kerosene, diesel and soya bean, and could efficiently degrade diesel.
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spelling pubmed-85117742021-10-15 Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites Ali, Ferdausi Das, Sharup Hossain, Tanim Jabid Chowdhury, Sumaiya Islam Zedny, Subrina Akter Das, Tuhin Ahmed Chowdhury, Mohammad Nazmul Uddin, Mohammad Seraj R Soc Open Sci Organismal and Evolutionary Biology Oil pollution is of increasing concern for environmental safety and the use of microbial surfactants in oil remediation has become inevitable for their efficacy and ecofriendly nature. In this work, biosurfactants of bacteria isolated from oil-contaminated soil have been characterized. Four potent biosurfactant-producing strains (SD4, SD11, SD12 and SD13) were selected from 27 isolates based on drop collapse assay and emulsification index, and identified as species belonging to Bacillus, Burkholderia, Providencia and Klebsiella, revealed from their 16S rRNA gene-based analysis. Detailed morphological and biochemical characteristics of each selected isolate were determined. Their growth conditions for maximum biosurfactant production were optimized and found quite similar among the four isolates with a pH of 3.0 and temperature 37°C after 6 or 7 days of growth on kerosene. The biosurfactants of SD4, SD11 and SD12 appeared to be glycolipids and that of SD13 a lipopeptide. Emulsification activity of most of the biosurfactants was stable at low and high temperatures (4–100°C), a wide range of pH (2–10) and salt concentrations (2–7% NaCl). Each biosurfactant showed antimicrobial activity against two or more pathogenic bacteria. The biosurfactants were well-capable of emulsifying kerosene, diesel and soya bean, and could efficiently degrade diesel. The Royal Society 2021-10-13 /pmc/articles/PMC8511774/ /pubmed/34659780 http://dx.doi.org/10.1098/rsos.211003 Text en © 2021 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Organismal and Evolutionary Biology
Ali, Ferdausi
Das, Sharup
Hossain, Tanim Jabid
Chowdhury, Sumaiya Islam
Zedny, Subrina Akter
Das, Tuhin
Ahmed Chowdhury, Mohammad Nazmul
Uddin, Mohammad Seraj
Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites
title Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites
title_full Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites
title_fullStr Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites
title_full_unstemmed Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites
title_short Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites
title_sort production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites
topic Organismal and Evolutionary Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511774/
https://www.ncbi.nlm.nih.gov/pubmed/34659780
http://dx.doi.org/10.1098/rsos.211003
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