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The Fecal Microbiota of Dogs Switching to a Raw Diet Only Partially Converges to That of Wolves

The genomic signature of dog domestication reveals adaptation to a starch-rich diet compared with their ancestor wolves. Diet is a key element to shape gut microbial populations in a direct way as well as through coevolution with the host. We investigated the dynamics in the gut microbiota of dogs w...

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Autores principales: Xu, Jia, Becker, Anne A. M. J., Luo, Yu, Zhang, Wenfu, Ge, Bingqian, Leng, Chunqing, Wang, Guyue, Ding, Limin, Wang, Jianmei, Fu, Xiaoyu, Janssens, Geert P. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511826/
https://www.ncbi.nlm.nih.gov/pubmed/34659139
http://dx.doi.org/10.3389/fmicb.2021.701439
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author Xu, Jia
Becker, Anne A. M. J.
Luo, Yu
Zhang, Wenfu
Ge, Bingqian
Leng, Chunqing
Wang, Guyue
Ding, Limin
Wang, Jianmei
Fu, Xiaoyu
Janssens, Geert P. J.
author_facet Xu, Jia
Becker, Anne A. M. J.
Luo, Yu
Zhang, Wenfu
Ge, Bingqian
Leng, Chunqing
Wang, Guyue
Ding, Limin
Wang, Jianmei
Fu, Xiaoyu
Janssens, Geert P. J.
author_sort Xu, Jia
collection PubMed
description The genomic signature of dog domestication reveals adaptation to a starch-rich diet compared with their ancestor wolves. Diet is a key element to shape gut microbial populations in a direct way as well as through coevolution with the host. We investigated the dynamics in the gut microbiota of dogs when shifting from a starch-rich, processed kibble diet to a nature-like raw meat diet, using wolves as a wild reference. Six healthy wolves from a local zoo and six healthy American Staffordshire Terriers were included. Dogs were fed the same commercial kibble diet for at least 3 months before sampling at day 0 (DC), and then switched to a raw meat diet (the same diet as the wolves) for 28 days. Samples from the dogs were collected at day 1 (DR1), week 1 (DR7), 2 (DR14), 3 (DR21), and 4 (DR28). The data showed that the microbial population of dogs switched from kibble diet to raw diet shifts the gut microbiota closer to that of wolves, yet still showing distinct differences. At phylum level, raw meat consumption increased the relative abundance of Fusobacteria and Bacteroidetes at DR1, DR7, DR14, and DR21 (q < 0.05) compared with DC, whereas no differences in these two phyla were observed between DC and DR28. At genus level, Faecalibacterium, Catenibacterium, Allisonella, and Megamonas were significantly lower in dogs consuming the raw diet from the first week onward and in wolves compared with dogs on the kibble diet. Linear discriminant analysis effect size (LEfSe) showed a higher abundance of Stenotrophomonas, Faecalibacterium, Megamonas, and Lactobacillus in dogs fed kibble diet compared with dogs fed raw diet for 28 days and wolves. In addition, wolves had greater unidentified Lachnospiraceae compared with dogs irrespective of the diets. These results suggested that carbohydrate-fermenting bacteria give way to protein fermenters when the diet is shifted from kibble to raw diet. In conclusion, some microbial phyla, families, and genera in dogs showed only temporary change upon dietary shift, whereas some microbial groups moved toward the microbial profile of wolves. These findings open the discussion on the extent of coevolution of the core microbiota of dogs throughout domestication.
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spelling pubmed-85118262021-10-14 The Fecal Microbiota of Dogs Switching to a Raw Diet Only Partially Converges to That of Wolves Xu, Jia Becker, Anne A. M. J. Luo, Yu Zhang, Wenfu Ge, Bingqian Leng, Chunqing Wang, Guyue Ding, Limin Wang, Jianmei Fu, Xiaoyu Janssens, Geert P. J. Front Microbiol Microbiology The genomic signature of dog domestication reveals adaptation to a starch-rich diet compared with their ancestor wolves. Diet is a key element to shape gut microbial populations in a direct way as well as through coevolution with the host. We investigated the dynamics in the gut microbiota of dogs when shifting from a starch-rich, processed kibble diet to a nature-like raw meat diet, using wolves as a wild reference. Six healthy wolves from a local zoo and six healthy American Staffordshire Terriers were included. Dogs were fed the same commercial kibble diet for at least 3 months before sampling at day 0 (DC), and then switched to a raw meat diet (the same diet as the wolves) for 28 days. Samples from the dogs were collected at day 1 (DR1), week 1 (DR7), 2 (DR14), 3 (DR21), and 4 (DR28). The data showed that the microbial population of dogs switched from kibble diet to raw diet shifts the gut microbiota closer to that of wolves, yet still showing distinct differences. At phylum level, raw meat consumption increased the relative abundance of Fusobacteria and Bacteroidetes at DR1, DR7, DR14, and DR21 (q < 0.05) compared with DC, whereas no differences in these two phyla were observed between DC and DR28. At genus level, Faecalibacterium, Catenibacterium, Allisonella, and Megamonas were significantly lower in dogs consuming the raw diet from the first week onward and in wolves compared with dogs on the kibble diet. Linear discriminant analysis effect size (LEfSe) showed a higher abundance of Stenotrophomonas, Faecalibacterium, Megamonas, and Lactobacillus in dogs fed kibble diet compared with dogs fed raw diet for 28 days and wolves. In addition, wolves had greater unidentified Lachnospiraceae compared with dogs irrespective of the diets. These results suggested that carbohydrate-fermenting bacteria give way to protein fermenters when the diet is shifted from kibble to raw diet. In conclusion, some microbial phyla, families, and genera in dogs showed only temporary change upon dietary shift, whereas some microbial groups moved toward the microbial profile of wolves. These findings open the discussion on the extent of coevolution of the core microbiota of dogs throughout domestication. Frontiers Media S.A. 2021-09-29 /pmc/articles/PMC8511826/ /pubmed/34659139 http://dx.doi.org/10.3389/fmicb.2021.701439 Text en Copyright © 2021 Xu, Becker, Luo, Zhang, Ge, Leng, Wang, Ding, Wang, Fu and Janssens. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xu, Jia
Becker, Anne A. M. J.
Luo, Yu
Zhang, Wenfu
Ge, Bingqian
Leng, Chunqing
Wang, Guyue
Ding, Limin
Wang, Jianmei
Fu, Xiaoyu
Janssens, Geert P. J.
The Fecal Microbiota of Dogs Switching to a Raw Diet Only Partially Converges to That of Wolves
title The Fecal Microbiota of Dogs Switching to a Raw Diet Only Partially Converges to That of Wolves
title_full The Fecal Microbiota of Dogs Switching to a Raw Diet Only Partially Converges to That of Wolves
title_fullStr The Fecal Microbiota of Dogs Switching to a Raw Diet Only Partially Converges to That of Wolves
title_full_unstemmed The Fecal Microbiota of Dogs Switching to a Raw Diet Only Partially Converges to That of Wolves
title_short The Fecal Microbiota of Dogs Switching to a Raw Diet Only Partially Converges to That of Wolves
title_sort fecal microbiota of dogs switching to a raw diet only partially converges to that of wolves
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511826/
https://www.ncbi.nlm.nih.gov/pubmed/34659139
http://dx.doi.org/10.3389/fmicb.2021.701439
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