A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages

Herein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol–gel matrix. Working parameters such as applied potential, pH, NAD(+) concentrati...

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Detalles Bibliográficos
Autores principales: Istrate, Oana Maria, Rotariu, Lucian, Bala, Camelia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512042/
https://www.ncbi.nlm.nih.gov/pubmed/34640829
http://dx.doi.org/10.3390/s21196510
Descripción
Sumario:Herein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol–gel matrix. Working parameters such as applied potential, pH, NAD(+) concentration, storage conditions were optimized. A response range between 0.05 and 2 mM was found with a sensitivity of 13.45 ± 0.67 µA/mM·cm(2) and a detection limit of 20 µM. The developed biosensor was used to detect ethanol in commercial beverages with good accuracy.

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