A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages

Herein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol–gel matrix. Working parameters such as applied potential, pH, NAD(+) concentrati...

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Detalles Bibliográficos
Autores principales: Istrate, Oana Maria, Rotariu, Lucian, Bala, Camelia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512042/
https://www.ncbi.nlm.nih.gov/pubmed/34640829
http://dx.doi.org/10.3390/s21196510
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author Istrate, Oana Maria
Rotariu, Lucian
Bala, Camelia
author_facet Istrate, Oana Maria
Rotariu, Lucian
Bala, Camelia
author_sort Istrate, Oana Maria
collection PubMed
description Herein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol–gel matrix. Working parameters such as applied potential, pH, NAD(+) concentration, storage conditions were optimized. A response range between 0.05 and 2 mM was found with a sensitivity of 13.45 ± 0.67 µA/mM·cm(2) and a detection limit of 20 µM. The developed biosensor was used to detect ethanol in commercial beverages with good accuracy.
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spelling pubmed-85120422021-10-14 A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages Istrate, Oana Maria Rotariu, Lucian Bala, Camelia Sensors (Basel) Article Herein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol–gel matrix. Working parameters such as applied potential, pH, NAD(+) concentration, storage conditions were optimized. A response range between 0.05 and 2 mM was found with a sensitivity of 13.45 ± 0.67 µA/mM·cm(2) and a detection limit of 20 µM. The developed biosensor was used to detect ethanol in commercial beverages with good accuracy. MDPI 2021-09-29 /pmc/articles/PMC8512042/ /pubmed/34640829 http://dx.doi.org/10.3390/s21196510 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Istrate, Oana Maria
Rotariu, Lucian
Bala, Camelia
A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages
title A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages
title_full A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages
title_fullStr A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages
title_full_unstemmed A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages
title_short A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages
title_sort novel amperometric biosensor based on poly(allylamine hydrochloride) for determination of ethanol in beverages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512042/
https://www.ncbi.nlm.nih.gov/pubmed/34640829
http://dx.doi.org/10.3390/s21196510
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