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In Vitro Multiplication and NMR Fingerprinting of Rare Veronica caucasica M. Bieb
Micropropagation of rare Veronica caucasica M. Bieb. was achieved by successful in vitro cultivation of mono-nodal segments on MS medium supplemented with 1.0 mg L(–1) 6-benzylaminopurine (BA) and then transferring the regenerated plants on hormone free basal MS medium for root development. In vitro...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512125/ https://www.ncbi.nlm.nih.gov/pubmed/34641432 http://dx.doi.org/10.3390/molecules26195888 |
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author | Mantovska, Desislava I. Zhiponova, Miroslava K. Georgiev, Milen I. Grozdanova, Tsvetinka Gerginova, Dessislava Alipieva, Kalina Simova, Svetlana Popova, Milena Kapchina-Toteva, Veneta M. Yordanova, Zhenya P. |
author_facet | Mantovska, Desislava I. Zhiponova, Miroslava K. Georgiev, Milen I. Grozdanova, Tsvetinka Gerginova, Dessislava Alipieva, Kalina Simova, Svetlana Popova, Milena Kapchina-Toteva, Veneta M. Yordanova, Zhenya P. |
author_sort | Mantovska, Desislava I. |
collection | PubMed |
description | Micropropagation of rare Veronica caucasica M. Bieb. was achieved by successful in vitro cultivation of mono-nodal segments on MS medium supplemented with 1.0 mg L(–1) 6-benzylaminopurine (BA) and then transferring the regenerated plants on hormone free basal MS medium for root development. In vitro multiplicated plants were successively acclimated in a growth chamber and a greenhouse with 92% survival. The number of plastid pigments and the total phenolics content in in vitro cultivated and ex vitro adapted plants were unchanged, and no accumulation of reactive oxygen species (ROS) was detected by staining with 3-3′-diaminobenzidine (DAB) and 2′,7′-dichlorofluorescein diacetate (DCF-DA). Nuclear Magnetic Resonance (NMR) fingerprinting allowed for the identification of the major alterations in metabolome of V. caucasica plants during the process of ex situ conservation. Iridoid glucosides such as verproside, aucubin and catalpol were characteristic for in vitro cultivated plants, while in ex vitro acclimated plants phenolic acid–protocatechuic acid and caffeic acid appeared dominant. The successful initiation of in vitro and ex vitro cultures is an alternative biotechnological approach for the preservation of V. caucasica and would allow for further studies of the biosynthetic potential of the species and the selection of lines with a high content of pharmaceutically valuable molecules and nutraceuticals. |
format | Online Article Text |
id | pubmed-8512125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85121252021-10-14 In Vitro Multiplication and NMR Fingerprinting of Rare Veronica caucasica M. Bieb Mantovska, Desislava I. Zhiponova, Miroslava K. Georgiev, Milen I. Grozdanova, Tsvetinka Gerginova, Dessislava Alipieva, Kalina Simova, Svetlana Popova, Milena Kapchina-Toteva, Veneta M. Yordanova, Zhenya P. Molecules Article Micropropagation of rare Veronica caucasica M. Bieb. was achieved by successful in vitro cultivation of mono-nodal segments on MS medium supplemented with 1.0 mg L(–1) 6-benzylaminopurine (BA) and then transferring the regenerated plants on hormone free basal MS medium for root development. In vitro multiplicated plants were successively acclimated in a growth chamber and a greenhouse with 92% survival. The number of plastid pigments and the total phenolics content in in vitro cultivated and ex vitro adapted plants were unchanged, and no accumulation of reactive oxygen species (ROS) was detected by staining with 3-3′-diaminobenzidine (DAB) and 2′,7′-dichlorofluorescein diacetate (DCF-DA). Nuclear Magnetic Resonance (NMR) fingerprinting allowed for the identification of the major alterations in metabolome of V. caucasica plants during the process of ex situ conservation. Iridoid glucosides such as verproside, aucubin and catalpol were characteristic for in vitro cultivated plants, while in ex vitro acclimated plants phenolic acid–protocatechuic acid and caffeic acid appeared dominant. The successful initiation of in vitro and ex vitro cultures is an alternative biotechnological approach for the preservation of V. caucasica and would allow for further studies of the biosynthetic potential of the species and the selection of lines with a high content of pharmaceutically valuable molecules and nutraceuticals. MDPI 2021-09-28 /pmc/articles/PMC8512125/ /pubmed/34641432 http://dx.doi.org/10.3390/molecules26195888 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mantovska, Desislava I. Zhiponova, Miroslava K. Georgiev, Milen I. Grozdanova, Tsvetinka Gerginova, Dessislava Alipieva, Kalina Simova, Svetlana Popova, Milena Kapchina-Toteva, Veneta M. Yordanova, Zhenya P. In Vitro Multiplication and NMR Fingerprinting of Rare Veronica caucasica M. Bieb |
title | In Vitro Multiplication and NMR Fingerprinting of Rare Veronica caucasica M. Bieb |
title_full | In Vitro Multiplication and NMR Fingerprinting of Rare Veronica caucasica M. Bieb |
title_fullStr | In Vitro Multiplication and NMR Fingerprinting of Rare Veronica caucasica M. Bieb |
title_full_unstemmed | In Vitro Multiplication and NMR Fingerprinting of Rare Veronica caucasica M. Bieb |
title_short | In Vitro Multiplication and NMR Fingerprinting of Rare Veronica caucasica M. Bieb |
title_sort | in vitro multiplication and nmr fingerprinting of rare veronica caucasica m. bieb |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512125/ https://www.ncbi.nlm.nih.gov/pubmed/34641432 http://dx.doi.org/10.3390/molecules26195888 |
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