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Dysregulation of NF-κB-Associated LncRNAs in Autism Spectrum Disorder

Autism spectrum disorder (ASD) is a long-standing neurodevelopmental condition with prominent effects on social behavior of affected children. This disorder has been linked with neuroinflammatory responses. NF-κB has been shown to affect these responses in the orbitofrontal cortex of patients with A...

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Autores principales: Honarmand Tamizkar, Kasra, Badrlou, Elham, Aslani, Termeh, Brand, Serge, Arsang-Jang, Shahram, Ghafouri-Fard, Soudeh, Taheri, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512169/
https://www.ncbi.nlm.nih.gov/pubmed/34658787
http://dx.doi.org/10.3389/fnmol.2021.747785
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author Honarmand Tamizkar, Kasra
Badrlou, Elham
Aslani, Termeh
Brand, Serge
Arsang-Jang, Shahram
Ghafouri-Fard, Soudeh
Taheri, Mohammad
author_facet Honarmand Tamizkar, Kasra
Badrlou, Elham
Aslani, Termeh
Brand, Serge
Arsang-Jang, Shahram
Ghafouri-Fard, Soudeh
Taheri, Mohammad
author_sort Honarmand Tamizkar, Kasra
collection PubMed
description Autism spectrum disorder (ASD) is a long-standing neurodevelopmental condition with prominent effects on social behavior of affected children. This disorder has been linked with neuroinflammatory responses. NF-κB has been shown to affect these responses in the orbitofrontal cortex of patients with ASD, thus being implicated in the pathogenesis of ASD. We measured expression of some NF-κB-associated lncRNAs and mRNAs (DILC, ANRIL, PACER, CHAST, ADINR, DICER1-AS1, HNF1A-AS1, NKILA, ATG5 and CEBPA) in the peripheral blood of ASD kids vs. healthy children. Expression quantities of ADINR, ANRIL, DILC, NKILA and CHAST were meaningfully higher in ASD cases compared with healthy kids (Posterior Beta = 1.402, P value < 0.0001; Posterior Beta = 2.959, P value < 0.0001; Posterior Beta = 0.882, P value = 0.012; Posterior Beta = 1.461, P value < 0.0001; Posterior Beta = 0.541, P value = 0.043, respectively). The Bonferroni corrected P values for these lncRNAs remained significant except for CHAST and DILC. Expression levels of other genes were not considerably different between cases and controls. Expressions of ATG5, DICER-AS1 and DILC were correlated with age of ASD patients (P < 0.0001). Among ASD cases, the most robust correlation has been detected between ADINR and NKILA (r = 0.87, P < 0.0001). Expression of none of genes has been correlated with age of healthy children. Among this group of children, expression levels of ADINR and CHAST were robustly correlated (r = 0.83, P < 0.0001). ANRIL had the greatest AUC value (AUC = 0.857), thus the best diagnostic power among the assessed genes. NKILA ranked the second position in this regard (AUC = 0.757). Thus, NF-κB-associated lncRNAs might partake in the pathogenesis of ASD.
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spelling pubmed-85121692021-10-14 Dysregulation of NF-κB-Associated LncRNAs in Autism Spectrum Disorder Honarmand Tamizkar, Kasra Badrlou, Elham Aslani, Termeh Brand, Serge Arsang-Jang, Shahram Ghafouri-Fard, Soudeh Taheri, Mohammad Front Mol Neurosci Molecular Neuroscience Autism spectrum disorder (ASD) is a long-standing neurodevelopmental condition with prominent effects on social behavior of affected children. This disorder has been linked with neuroinflammatory responses. NF-κB has been shown to affect these responses in the orbitofrontal cortex of patients with ASD, thus being implicated in the pathogenesis of ASD. We measured expression of some NF-κB-associated lncRNAs and mRNAs (DILC, ANRIL, PACER, CHAST, ADINR, DICER1-AS1, HNF1A-AS1, NKILA, ATG5 and CEBPA) in the peripheral blood of ASD kids vs. healthy children. Expression quantities of ADINR, ANRIL, DILC, NKILA and CHAST were meaningfully higher in ASD cases compared with healthy kids (Posterior Beta = 1.402, P value < 0.0001; Posterior Beta = 2.959, P value < 0.0001; Posterior Beta = 0.882, P value = 0.012; Posterior Beta = 1.461, P value < 0.0001; Posterior Beta = 0.541, P value = 0.043, respectively). The Bonferroni corrected P values for these lncRNAs remained significant except for CHAST and DILC. Expression levels of other genes were not considerably different between cases and controls. Expressions of ATG5, DICER-AS1 and DILC were correlated with age of ASD patients (P < 0.0001). Among ASD cases, the most robust correlation has been detected between ADINR and NKILA (r = 0.87, P < 0.0001). Expression of none of genes has been correlated with age of healthy children. Among this group of children, expression levels of ADINR and CHAST were robustly correlated (r = 0.83, P < 0.0001). ANRIL had the greatest AUC value (AUC = 0.857), thus the best diagnostic power among the assessed genes. NKILA ranked the second position in this regard (AUC = 0.757). Thus, NF-κB-associated lncRNAs might partake in the pathogenesis of ASD. Frontiers Media S.A. 2021-09-28 /pmc/articles/PMC8512169/ /pubmed/34658787 http://dx.doi.org/10.3389/fnmol.2021.747785 Text en Copyright © 2021 Honarmand Tamizkar, Badrlou, Aslani, Brand, Arsang-Jang, Ghafouri-Fard and Taheri. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Neuroscience
Honarmand Tamizkar, Kasra
Badrlou, Elham
Aslani, Termeh
Brand, Serge
Arsang-Jang, Shahram
Ghafouri-Fard, Soudeh
Taheri, Mohammad
Dysregulation of NF-κB-Associated LncRNAs in Autism Spectrum Disorder
title Dysregulation of NF-κB-Associated LncRNAs in Autism Spectrum Disorder
title_full Dysregulation of NF-κB-Associated LncRNAs in Autism Spectrum Disorder
title_fullStr Dysregulation of NF-κB-Associated LncRNAs in Autism Spectrum Disorder
title_full_unstemmed Dysregulation of NF-κB-Associated LncRNAs in Autism Spectrum Disorder
title_short Dysregulation of NF-κB-Associated LncRNAs in Autism Spectrum Disorder
title_sort dysregulation of nf-κb-associated lncrnas in autism spectrum disorder
topic Molecular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512169/
https://www.ncbi.nlm.nih.gov/pubmed/34658787
http://dx.doi.org/10.3389/fnmol.2021.747785
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