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Poly-ε-Caprolactone/Fibrin-Alginate Scaffold: A New Pro-Angiogenic Composite Biomaterial for the Treatment of Bone Defects

We hypothesized that a composite of 3D porous melt-electrowritten poly-ɛ-caprolactone (PCL) coated throughout with a porous and slowly biodegradable fibrin/alginate (FA) matrix would accelerate bone repair due to its angiogenic potential. Scanning electron microscopy showed that the open pore struct...

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Autores principales: Ren, Jiongyu, Kohli, Nupur, Sharma, Vaibhav, Shakouri, Taleen, Keskin-Erdogan, Zalike, Saifzadeh, Siamak, Brierly, Gary I., Knowles, Jonathan C., Woodruff, Maria A., García-Gareta, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512525/
https://www.ncbi.nlm.nih.gov/pubmed/34641215
http://dx.doi.org/10.3390/polym13193399
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author Ren, Jiongyu
Kohli, Nupur
Sharma, Vaibhav
Shakouri, Taleen
Keskin-Erdogan, Zalike
Saifzadeh, Siamak
Brierly, Gary I.
Knowles, Jonathan C.
Woodruff, Maria A.
García-Gareta, Elena
author_facet Ren, Jiongyu
Kohli, Nupur
Sharma, Vaibhav
Shakouri, Taleen
Keskin-Erdogan, Zalike
Saifzadeh, Siamak
Brierly, Gary I.
Knowles, Jonathan C.
Woodruff, Maria A.
García-Gareta, Elena
author_sort Ren, Jiongyu
collection PubMed
description We hypothesized that a composite of 3D porous melt-electrowritten poly-ɛ-caprolactone (PCL) coated throughout with a porous and slowly biodegradable fibrin/alginate (FA) matrix would accelerate bone repair due to its angiogenic potential. Scanning electron microscopy showed that the open pore structure of the FA matrix was maintained in the PCL/FA composites. Fourier transform infrared spectroscopy and differential scanning calorimetry showed complete coverage of the PCL fibres by FA, and the PCL/FA crystallinity was decreased compared with PCL. In vitro cell work with osteoprogenitor cells showed that they preferentially bound to the FA component and proliferated on all scaffolds over 28 days. A chorioallantoic membrane assay showed more blood vessel infiltration into FA and PCL/FA compared with PCL, and a significantly higher number of bifurcation points for PCL/FA compared with both FA and PCL. Implantation into a rat cranial defect model followed by microcomputed tomography, histology, and immunohistochemistry after 4- and 12-weeks post operation showed fast early bone formation at week 4, with significantly higher bone formation for FA and PCL/FA compared with PCL. However, this phenomenon was not extrapolated to week 12. Therefore, for long-term bone regeneration, tuning of FA degradation to ensure syncing with new bone formation is likely necessary.
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spelling pubmed-85125252021-10-14 Poly-ε-Caprolactone/Fibrin-Alginate Scaffold: A New Pro-Angiogenic Composite Biomaterial for the Treatment of Bone Defects Ren, Jiongyu Kohli, Nupur Sharma, Vaibhav Shakouri, Taleen Keskin-Erdogan, Zalike Saifzadeh, Siamak Brierly, Gary I. Knowles, Jonathan C. Woodruff, Maria A. García-Gareta, Elena Polymers (Basel) Article We hypothesized that a composite of 3D porous melt-electrowritten poly-ɛ-caprolactone (PCL) coated throughout with a porous and slowly biodegradable fibrin/alginate (FA) matrix would accelerate bone repair due to its angiogenic potential. Scanning electron microscopy showed that the open pore structure of the FA matrix was maintained in the PCL/FA composites. Fourier transform infrared spectroscopy and differential scanning calorimetry showed complete coverage of the PCL fibres by FA, and the PCL/FA crystallinity was decreased compared with PCL. In vitro cell work with osteoprogenitor cells showed that they preferentially bound to the FA component and proliferated on all scaffolds over 28 days. A chorioallantoic membrane assay showed more blood vessel infiltration into FA and PCL/FA compared with PCL, and a significantly higher number of bifurcation points for PCL/FA compared with both FA and PCL. Implantation into a rat cranial defect model followed by microcomputed tomography, histology, and immunohistochemistry after 4- and 12-weeks post operation showed fast early bone formation at week 4, with significantly higher bone formation for FA and PCL/FA compared with PCL. However, this phenomenon was not extrapolated to week 12. Therefore, for long-term bone regeneration, tuning of FA degradation to ensure syncing with new bone formation is likely necessary. MDPI 2021-10-02 /pmc/articles/PMC8512525/ /pubmed/34641215 http://dx.doi.org/10.3390/polym13193399 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ren, Jiongyu
Kohli, Nupur
Sharma, Vaibhav
Shakouri, Taleen
Keskin-Erdogan, Zalike
Saifzadeh, Siamak
Brierly, Gary I.
Knowles, Jonathan C.
Woodruff, Maria A.
García-Gareta, Elena
Poly-ε-Caprolactone/Fibrin-Alginate Scaffold: A New Pro-Angiogenic Composite Biomaterial for the Treatment of Bone Defects
title Poly-ε-Caprolactone/Fibrin-Alginate Scaffold: A New Pro-Angiogenic Composite Biomaterial for the Treatment of Bone Defects
title_full Poly-ε-Caprolactone/Fibrin-Alginate Scaffold: A New Pro-Angiogenic Composite Biomaterial for the Treatment of Bone Defects
title_fullStr Poly-ε-Caprolactone/Fibrin-Alginate Scaffold: A New Pro-Angiogenic Composite Biomaterial for the Treatment of Bone Defects
title_full_unstemmed Poly-ε-Caprolactone/Fibrin-Alginate Scaffold: A New Pro-Angiogenic Composite Biomaterial for the Treatment of Bone Defects
title_short Poly-ε-Caprolactone/Fibrin-Alginate Scaffold: A New Pro-Angiogenic Composite Biomaterial for the Treatment of Bone Defects
title_sort poly-ε-caprolactone/fibrin-alginate scaffold: a new pro-angiogenic composite biomaterial for the treatment of bone defects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512525/
https://www.ncbi.nlm.nih.gov/pubmed/34641215
http://dx.doi.org/10.3390/polym13193399
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