Cargando…

Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis

Fentanyl and fentalogs’ intake as drugs of abuse is experiencing a great increase in recent years. For this reason, there are more and more cases in which it is important to recognize and quantify these molecules and related metabolites in biological matrices. Oral fluid (OF) is often used to find o...

Descripción completa

Detalles Bibliográficos
Autores principales: Vincenti, Flaminia, Montesano, Camilla, Pirau, Svetlana, Gregori, Adolfo, Di Rosa, Fabiana, Curini, Roberta, Sergi, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512571/
https://www.ncbi.nlm.nih.gov/pubmed/34641416
http://dx.doi.org/10.3390/molecules26195870
_version_ 1784583025607049216
author Vincenti, Flaminia
Montesano, Camilla
Pirau, Svetlana
Gregori, Adolfo
Di Rosa, Fabiana
Curini, Roberta
Sergi, Manuel
author_facet Vincenti, Flaminia
Montesano, Camilla
Pirau, Svetlana
Gregori, Adolfo
Di Rosa, Fabiana
Curini, Roberta
Sergi, Manuel
author_sort Vincenti, Flaminia
collection PubMed
description Fentanyl and fentalogs’ intake as drugs of abuse is experiencing a great increase in recent years. For this reason, there are more and more cases in which it is important to recognize and quantify these molecules and related metabolites in biological matrices. Oral fluid (OF) is often used to find out if a subject has recently used a psychoactive substance and if, therefore, the person is still under the effect of psychotropics. Given its difficulty in handling, good sample preparation and the development of instrumental methods for analysis are essential. In this work, an analytical method is proposed for the simultaneous determination of 25 analytes, including fentanyl, several derivatives and metabolites. OF was collected by means of passive drool; sample pretreatment was developed in order to be fast, simple and possibly semi-automated by exploiting microextraction on packed sorbent (MEPS). The analysis was performed by means of LC–HRMS/MS obtaining good identification and quantification of all the analytes in less than 10 min. The proposed method was fully validated according to the Scientific Working Group for Forensic Toxicology (SWGTOX) international guidelines. Good results were obtained in terms of recoveries, matrix effect and sensitivity, showing that this method could represent a useful tool in forensic toxicology. The presented method was successfully applied to the analysis of proficiency test samples.
format Online
Article
Text
id pubmed-8512571
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85125712021-10-14 Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis Vincenti, Flaminia Montesano, Camilla Pirau, Svetlana Gregori, Adolfo Di Rosa, Fabiana Curini, Roberta Sergi, Manuel Molecules Article Fentanyl and fentalogs’ intake as drugs of abuse is experiencing a great increase in recent years. For this reason, there are more and more cases in which it is important to recognize and quantify these molecules and related metabolites in biological matrices. Oral fluid (OF) is often used to find out if a subject has recently used a psychoactive substance and if, therefore, the person is still under the effect of psychotropics. Given its difficulty in handling, good sample preparation and the development of instrumental methods for analysis are essential. In this work, an analytical method is proposed for the simultaneous determination of 25 analytes, including fentanyl, several derivatives and metabolites. OF was collected by means of passive drool; sample pretreatment was developed in order to be fast, simple and possibly semi-automated by exploiting microextraction on packed sorbent (MEPS). The analysis was performed by means of LC–HRMS/MS obtaining good identification and quantification of all the analytes in less than 10 min. The proposed method was fully validated according to the Scientific Working Group for Forensic Toxicology (SWGTOX) international guidelines. Good results were obtained in terms of recoveries, matrix effect and sensitivity, showing that this method could represent a useful tool in forensic toxicology. The presented method was successfully applied to the analysis of proficiency test samples. MDPI 2021-09-28 /pmc/articles/PMC8512571/ /pubmed/34641416 http://dx.doi.org/10.3390/molecules26195870 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vincenti, Flaminia
Montesano, Camilla
Pirau, Svetlana
Gregori, Adolfo
Di Rosa, Fabiana
Curini, Roberta
Sergi, Manuel
Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis
title Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis
title_full Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis
title_fullStr Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis
title_full_unstemmed Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis
title_short Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis
title_sort simultaneous quantification of 25 fentanyl derivatives and metabolites in oral fluid by means of microextraction on packed sorbent and lc–hrms/ms analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512571/
https://www.ncbi.nlm.nih.gov/pubmed/34641416
http://dx.doi.org/10.3390/molecules26195870
work_keys_str_mv AT vincentiflaminia simultaneousquantificationof25fentanylderivativesandmetabolitesinoralfluidbymeansofmicroextractiononpackedsorbentandlchrmsmsanalysis
AT montesanocamilla simultaneousquantificationof25fentanylderivativesandmetabolitesinoralfluidbymeansofmicroextractiononpackedsorbentandlchrmsmsanalysis
AT pirausvetlana simultaneousquantificationof25fentanylderivativesandmetabolitesinoralfluidbymeansofmicroextractiononpackedsorbentandlchrmsmsanalysis
AT gregoriadolfo simultaneousquantificationof25fentanylderivativesandmetabolitesinoralfluidbymeansofmicroextractiononpackedsorbentandlchrmsmsanalysis
AT dirosafabiana simultaneousquantificationof25fentanylderivativesandmetabolitesinoralfluidbymeansofmicroextractiononpackedsorbentandlchrmsmsanalysis
AT curiniroberta simultaneousquantificationof25fentanylderivativesandmetabolitesinoralfluidbymeansofmicroextractiononpackedsorbentandlchrmsmsanalysis
AT sergimanuel simultaneousquantificationof25fentanylderivativesandmetabolitesinoralfluidbymeansofmicroextractiononpackedsorbentandlchrmsmsanalysis