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Recent advances in sensitivity enhancement for lateral flow assay

Conventional lateral flow assay (LFA) is typically performed by observing the color changes in the test lines by naked eyes, which achieves considerable commercial success and has a significant impact on the fields of food safety, environment monitoring, disease diagnosis, and other applications. Ho...

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Detalles Bibliográficos
Autores principales: Deng, Yulin, Jiang, Hao, Li, Xiaoqiong, Lv, Xuefei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513549/
https://www.ncbi.nlm.nih.gov/pubmed/34647157
http://dx.doi.org/10.1007/s00604-021-05037-z
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author Deng, Yulin
Jiang, Hao
Li, Xiaoqiong
Lv, Xuefei
author_facet Deng, Yulin
Jiang, Hao
Li, Xiaoqiong
Lv, Xuefei
author_sort Deng, Yulin
collection PubMed
description Conventional lateral flow assay (LFA) is typically performed by observing the color changes in the test lines by naked eyes, which achieves considerable commercial success and has a significant impact on the fields of food safety, environment monitoring, disease diagnosis, and other applications. However, this qualitative detection method is not very suitable for low levels of disease biomarkers’ detection. Although many nanomaterials are used as new labels for LFA, additional readers limit their application to some extent. Fortunately, a lot of work has been done for improving the sensitivity of LFA. In this review, currently reported LFA sensitivity enhancement methods with an objective evaluation are summarized, such as sample pretreatment, the change of flow rate, and label evolution, and future development direction and challenges of LFAs are discussed.
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spelling pubmed-85135492021-10-13 Recent advances in sensitivity enhancement for lateral flow assay Deng, Yulin Jiang, Hao Li, Xiaoqiong Lv, Xuefei Mikrochim Acta Review Article Conventional lateral flow assay (LFA) is typically performed by observing the color changes in the test lines by naked eyes, which achieves considerable commercial success and has a significant impact on the fields of food safety, environment monitoring, disease diagnosis, and other applications. However, this qualitative detection method is not very suitable for low levels of disease biomarkers’ detection. Although many nanomaterials are used as new labels for LFA, additional readers limit their application to some extent. Fortunately, a lot of work has been done for improving the sensitivity of LFA. In this review, currently reported LFA sensitivity enhancement methods with an objective evaluation are summarized, such as sample pretreatment, the change of flow rate, and label evolution, and future development direction and challenges of LFAs are discussed. Springer Vienna 2021-10-13 2021 /pmc/articles/PMC8513549/ /pubmed/34647157 http://dx.doi.org/10.1007/s00604-021-05037-z Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Review Article
Deng, Yulin
Jiang, Hao
Li, Xiaoqiong
Lv, Xuefei
Recent advances in sensitivity enhancement for lateral flow assay
title Recent advances in sensitivity enhancement for lateral flow assay
title_full Recent advances in sensitivity enhancement for lateral flow assay
title_fullStr Recent advances in sensitivity enhancement for lateral flow assay
title_full_unstemmed Recent advances in sensitivity enhancement for lateral flow assay
title_short Recent advances in sensitivity enhancement for lateral flow assay
title_sort recent advances in sensitivity enhancement for lateral flow assay
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513549/
https://www.ncbi.nlm.nih.gov/pubmed/34647157
http://dx.doi.org/10.1007/s00604-021-05037-z
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