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Recent advances in sensitivity enhancement for lateral flow assay
Conventional lateral flow assay (LFA) is typically performed by observing the color changes in the test lines by naked eyes, which achieves considerable commercial success and has a significant impact on the fields of food safety, environment monitoring, disease diagnosis, and other applications. Ho...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513549/ https://www.ncbi.nlm.nih.gov/pubmed/34647157 http://dx.doi.org/10.1007/s00604-021-05037-z |
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author | Deng, Yulin Jiang, Hao Li, Xiaoqiong Lv, Xuefei |
author_facet | Deng, Yulin Jiang, Hao Li, Xiaoqiong Lv, Xuefei |
author_sort | Deng, Yulin |
collection | PubMed |
description | Conventional lateral flow assay (LFA) is typically performed by observing the color changes in the test lines by naked eyes, which achieves considerable commercial success and has a significant impact on the fields of food safety, environment monitoring, disease diagnosis, and other applications. However, this qualitative detection method is not very suitable for low levels of disease biomarkers’ detection. Although many nanomaterials are used as new labels for LFA, additional readers limit their application to some extent. Fortunately, a lot of work has been done for improving the sensitivity of LFA. In this review, currently reported LFA sensitivity enhancement methods with an objective evaluation are summarized, such as sample pretreatment, the change of flow rate, and label evolution, and future development direction and challenges of LFAs are discussed. |
format | Online Article Text |
id | pubmed-8513549 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-85135492021-10-13 Recent advances in sensitivity enhancement for lateral flow assay Deng, Yulin Jiang, Hao Li, Xiaoqiong Lv, Xuefei Mikrochim Acta Review Article Conventional lateral flow assay (LFA) is typically performed by observing the color changes in the test lines by naked eyes, which achieves considerable commercial success and has a significant impact on the fields of food safety, environment monitoring, disease diagnosis, and other applications. However, this qualitative detection method is not very suitable for low levels of disease biomarkers’ detection. Although many nanomaterials are used as new labels for LFA, additional readers limit their application to some extent. Fortunately, a lot of work has been done for improving the sensitivity of LFA. In this review, currently reported LFA sensitivity enhancement methods with an objective evaluation are summarized, such as sample pretreatment, the change of flow rate, and label evolution, and future development direction and challenges of LFAs are discussed. Springer Vienna 2021-10-13 2021 /pmc/articles/PMC8513549/ /pubmed/34647157 http://dx.doi.org/10.1007/s00604-021-05037-z Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Review Article Deng, Yulin Jiang, Hao Li, Xiaoqiong Lv, Xuefei Recent advances in sensitivity enhancement for lateral flow assay |
title | Recent advances in sensitivity enhancement for lateral flow assay |
title_full | Recent advances in sensitivity enhancement for lateral flow assay |
title_fullStr | Recent advances in sensitivity enhancement for lateral flow assay |
title_full_unstemmed | Recent advances in sensitivity enhancement for lateral flow assay |
title_short | Recent advances in sensitivity enhancement for lateral flow assay |
title_sort | recent advances in sensitivity enhancement for lateral flow assay |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513549/ https://www.ncbi.nlm.nih.gov/pubmed/34647157 http://dx.doi.org/10.1007/s00604-021-05037-z |
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