A DNA-based voltmeter for organelles

The role of membrane potential in most intracellular organelles remains unexplored because of the lack of suitable tools. Here, we describe a fluorescent DNA-nanodevice that reports absolute membrane potential and can be targeted to organelles in live cells. This nanodevice, denoted Voltair, bears a...

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Autores principales: Saminathan, Anand, Devany, John, Veetil, Aneesh Tazhe, Suresh, Bhavyashree, Pillai, Kavya Smitha, Schwake, Michael, Krishnan, Yamuna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513801/
https://www.ncbi.nlm.nih.gov/pubmed/33139937
http://dx.doi.org/10.1038/s41565-020-00784-1
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author Saminathan, Anand
Devany, John
Veetil, Aneesh Tazhe
Suresh, Bhavyashree
Pillai, Kavya Smitha
Schwake, Michael
Krishnan, Yamuna
author_facet Saminathan, Anand
Devany, John
Veetil, Aneesh Tazhe
Suresh, Bhavyashree
Pillai, Kavya Smitha
Schwake, Michael
Krishnan, Yamuna
author_sort Saminathan, Anand
collection PubMed
description The role of membrane potential in most intracellular organelles remains unexplored because of the lack of suitable tools. Here, we describe a fluorescent DNA-nanodevice that reports absolute membrane potential and can be targeted to organelles in live cells. This nanodevice, denoted Voltair, bears a voltage-sensitive fluorophore, a reference fluorophore for ratiometry, and acts as an endocytic tracer. Using Voltair we could measure the membrane potential of different organelles in situ in live cells. Voltair can potentially guide the rational design of biocompatible electronics as well as expand our understanding of how membrane potential regulates organelle biology.
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spelling pubmed-85138012021-10-13 A DNA-based voltmeter for organelles Saminathan, Anand Devany, John Veetil, Aneesh Tazhe Suresh, Bhavyashree Pillai, Kavya Smitha Schwake, Michael Krishnan, Yamuna Nat Nanotechnol Article The role of membrane potential in most intracellular organelles remains unexplored because of the lack of suitable tools. Here, we describe a fluorescent DNA-nanodevice that reports absolute membrane potential and can be targeted to organelles in live cells. This nanodevice, denoted Voltair, bears a voltage-sensitive fluorophore, a reference fluorophore for ratiometry, and acts as an endocytic tracer. Using Voltair we could measure the membrane potential of different organelles in situ in live cells. Voltair can potentially guide the rational design of biocompatible electronics as well as expand our understanding of how membrane potential regulates organelle biology. 2020-11-02 2021-01 /pmc/articles/PMC8513801/ /pubmed/33139937 http://dx.doi.org/10.1038/s41565-020-00784-1 Text en http://www.nature.com/authors/editorial_policies/license.html#termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Saminathan, Anand
Devany, John
Veetil, Aneesh Tazhe
Suresh, Bhavyashree
Pillai, Kavya Smitha
Schwake, Michael
Krishnan, Yamuna
A DNA-based voltmeter for organelles
title A DNA-based voltmeter for organelles
title_full A DNA-based voltmeter for organelles
title_fullStr A DNA-based voltmeter for organelles
title_full_unstemmed A DNA-based voltmeter for organelles
title_short A DNA-based voltmeter for organelles
title_sort dna-based voltmeter for organelles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513801/
https://www.ncbi.nlm.nih.gov/pubmed/33139937
http://dx.doi.org/10.1038/s41565-020-00784-1
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