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Chlamydia buteonis in birds of prey presented to California wildlife rehabilitation facilities

Chlamydial infections, caused by a group of obligate, intracellular, gram-negative bacteria, have health implications for animals and humans. Due to their highly infectious nature and zoonotic potential, staff at wildlife rehabilitation centers should be educated on the clinical manifestations, prev...

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Autores principales: Seibert, Brittany A., Keel, Michael K., Kelly, Terra R., Nilsen, Roger A., Ciembor, Paula, Pesti, Denise, Gregory, Christopher R., Ritchie, Branson W., Hawkins, Michelle G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513822/
https://www.ncbi.nlm.nih.gov/pubmed/34644359
http://dx.doi.org/10.1371/journal.pone.0258500
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author Seibert, Brittany A.
Keel, Michael K.
Kelly, Terra R.
Nilsen, Roger A.
Ciembor, Paula
Pesti, Denise
Gregory, Christopher R.
Ritchie, Branson W.
Hawkins, Michelle G.
author_facet Seibert, Brittany A.
Keel, Michael K.
Kelly, Terra R.
Nilsen, Roger A.
Ciembor, Paula
Pesti, Denise
Gregory, Christopher R.
Ritchie, Branson W.
Hawkins, Michelle G.
author_sort Seibert, Brittany A.
collection PubMed
description Chlamydial infections, caused by a group of obligate, intracellular, gram-negative bacteria, have health implications for animals and humans. Due to their highly infectious nature and zoonotic potential, staff at wildlife rehabilitation centers should be educated on the clinical manifestations, prevalence, and risk factors associated with Chlamydia spp. infections in raptors. The objectives of this study were to document the prevalence of chlamydial DNA shedding and anti-chlamydial antibodies in raptors admitted to five wildlife rehabilitation centers in California over a one-year period. Chlamydial prevalence was estimated in raptors for each center and potential risk factors associated with infection were evaluated, including location, species, season, and age class. Plasma samples and conjunctiva/choana/cloaca swabs were collected for serology and qPCR from a subset of 263 birds of prey, representing 18 species. Serologic assays identified both anti-C. buteonis IgM and anti-chlamydial IgY antibodies. Chlamydial DNA and anti-chlamydial antibodies were detected in 4.18% (11/263) and 3.14% (6/191) of patients, respectively. Chamydial DNA was identified in raptors from the families Accipitridae and Strigidae while anti-C.buteonis IgM was identified in birds identified in Accipitridae, Falconidae, Strigidae, and Cathartidae. Two of the chlamydial DNA positive birds (one Swainson’s hawk (Buteo swainsoni) and one red-tailed hawk (Buteo jamaicensis)) were necropsied, and tissues were collected for culture. Sequencing of the cultured elementary bodies revealed a chlamydial DNA sequence with 99.97% average nucleotide identity to the recently described Chlamydia buteonis. Spatial clusters of seropositive raptors and raptors positive for chlamydial DNA were detected in northern California. Infections were most prevalent during the winter season. Furthermore, while the proportion of raptors testing positive for chlamydial DNA was similar across age classes, seroprevalence was highest in adults. This study questions the current knowledge on C. buteonis host range and highlights the importance of further studies to evaluate the diversity and epidemiology of Chlamydia spp. infecting raptor populations.
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spelling pubmed-85138222021-10-14 Chlamydia buteonis in birds of prey presented to California wildlife rehabilitation facilities Seibert, Brittany A. Keel, Michael K. Kelly, Terra R. Nilsen, Roger A. Ciembor, Paula Pesti, Denise Gregory, Christopher R. Ritchie, Branson W. Hawkins, Michelle G. PLoS One Research Article Chlamydial infections, caused by a group of obligate, intracellular, gram-negative bacteria, have health implications for animals and humans. Due to their highly infectious nature and zoonotic potential, staff at wildlife rehabilitation centers should be educated on the clinical manifestations, prevalence, and risk factors associated with Chlamydia spp. infections in raptors. The objectives of this study were to document the prevalence of chlamydial DNA shedding and anti-chlamydial antibodies in raptors admitted to five wildlife rehabilitation centers in California over a one-year period. Chlamydial prevalence was estimated in raptors for each center and potential risk factors associated with infection were evaluated, including location, species, season, and age class. Plasma samples and conjunctiva/choana/cloaca swabs were collected for serology and qPCR from a subset of 263 birds of prey, representing 18 species. Serologic assays identified both anti-C. buteonis IgM and anti-chlamydial IgY antibodies. Chlamydial DNA and anti-chlamydial antibodies were detected in 4.18% (11/263) and 3.14% (6/191) of patients, respectively. Chamydial DNA was identified in raptors from the families Accipitridae and Strigidae while anti-C.buteonis IgM was identified in birds identified in Accipitridae, Falconidae, Strigidae, and Cathartidae. Two of the chlamydial DNA positive birds (one Swainson’s hawk (Buteo swainsoni) and one red-tailed hawk (Buteo jamaicensis)) were necropsied, and tissues were collected for culture. Sequencing of the cultured elementary bodies revealed a chlamydial DNA sequence with 99.97% average nucleotide identity to the recently described Chlamydia buteonis. Spatial clusters of seropositive raptors and raptors positive for chlamydial DNA were detected in northern California. Infections were most prevalent during the winter season. Furthermore, while the proportion of raptors testing positive for chlamydial DNA was similar across age classes, seroprevalence was highest in adults. This study questions the current knowledge on C. buteonis host range and highlights the importance of further studies to evaluate the diversity and epidemiology of Chlamydia spp. infecting raptor populations. Public Library of Science 2021-10-13 /pmc/articles/PMC8513822/ /pubmed/34644359 http://dx.doi.org/10.1371/journal.pone.0258500 Text en © 2021 Seibert et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Seibert, Brittany A.
Keel, Michael K.
Kelly, Terra R.
Nilsen, Roger A.
Ciembor, Paula
Pesti, Denise
Gregory, Christopher R.
Ritchie, Branson W.
Hawkins, Michelle G.
Chlamydia buteonis in birds of prey presented to California wildlife rehabilitation facilities
title Chlamydia buteonis in birds of prey presented to California wildlife rehabilitation facilities
title_full Chlamydia buteonis in birds of prey presented to California wildlife rehabilitation facilities
title_fullStr Chlamydia buteonis in birds of prey presented to California wildlife rehabilitation facilities
title_full_unstemmed Chlamydia buteonis in birds of prey presented to California wildlife rehabilitation facilities
title_short Chlamydia buteonis in birds of prey presented to California wildlife rehabilitation facilities
title_sort chlamydia buteonis in birds of prey presented to california wildlife rehabilitation facilities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513822/
https://www.ncbi.nlm.nih.gov/pubmed/34644359
http://dx.doi.org/10.1371/journal.pone.0258500
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