Cargando…

Supplemental Dietary Selenohomolanthionine Improve Antioxidant Activity and Immune Function in Weaned Beagle Puppies

The purpose of this study was to investigate the effects of dietary Selenohomolanthionine (SeHLan) on antioxidant status and immune response in canine parvovirus (CPV) vaccinated puppies. In this study, 30 weaned puppies were randomly divided into six groups: control group (–Se/–Vacc), immunization...

Descripción completa

Detalles Bibliográficos
Autores principales: Shao, Chunyan, Zheng, Moufeng, Yu, Ziwei, Jiang, Sheng, Zhou, Bin, Song, Quanjiang, Ma, Tianning, Zhou, Yingshan, Dong, Wanyu, Li, Ding, Gu, Yao, Wang, Xiaodu, Song, Houhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8514779/
https://www.ncbi.nlm.nih.gov/pubmed/34660766
http://dx.doi.org/10.3389/fvets.2021.728358
_version_ 1784583469148405760
author Shao, Chunyan
Zheng, Moufeng
Yu, Ziwei
Jiang, Sheng
Zhou, Bin
Song, Quanjiang
Ma, Tianning
Zhou, Yingshan
Dong, Wanyu
Li, Ding
Gu, Yao
Wang, Xiaodu
Song, Houhui
author_facet Shao, Chunyan
Zheng, Moufeng
Yu, Ziwei
Jiang, Sheng
Zhou, Bin
Song, Quanjiang
Ma, Tianning
Zhou, Yingshan
Dong, Wanyu
Li, Ding
Gu, Yao
Wang, Xiaodu
Song, Houhui
author_sort Shao, Chunyan
collection PubMed
description The purpose of this study was to investigate the effects of dietary Selenohomolanthionine (SeHLan) on antioxidant status and immune response in canine parvovirus (CPV) vaccinated puppies. In this study, 30 weaned puppies were randomly divided into six groups: control group (–Se/–Vacc), immunization group (–Se/+Vacc), supplementation of sodium selenite group (SS/+Vacc, 0.35 mg/kg DM), low-dose SeHLan group (SeHLan-L/+Vacc, 0.35 mg/kg DM), mid-dose SeHLan group (SeHLan-M/+Vacc, 1.0 mg/kg DM), and high-dose SeHLan group (SeHLan-H/+Vacc, 2.0 mg/kg DM). The puppies were fed for 42 days and vaccinated with Vanguard Plus 5 on day 0 and day 21. Blood samples were collected on 7, 14, 21, 28, 35, 42 days post-immunization (PI) for determination of antioxidant indicators, lymphocyte proliferation index, serum cytokine concentration (IL-2, IL-4), canine polymorphonuclear neutrophils (PMN) phagocytic function, and the level of CPV antibody titers. The results showed that SeHLan supplementation raised the serum Se concentration and glutathione peroxidase (GSH-Px) activity in a dose-dependent manner (P < 0.05). It also increased the activity of serum superoxide dismutase (SOD) and decreased serum malondialdehyde (MDA) content, especially in SeHLan-M/+Vacc group (1.0 mg/kg DM) (P < 0.01). SeHLan supplementation significantly increased lymphocyte proliferation, IL-2, and IL-4 levels in canine serum, and enhanced phagocytosis of PMN in vaccinated puppies (P < 0.05). Moreover, SeHLan supplementation shortened the CPV antibody production time and increased the CPV antibody titers (P < 0.05). Of note, the beneficial effects of SeHLan were superior to those of SS. In conclusion, dietary SeHLan supplementation improved antioxidant activity, increased CPV antibody titers, and enhanced immune function in puppies after weaning. An appropriate dosage of SeHLan (1~2 mg/kg DM) may confer nutritional benefits in puppies.
format Online
Article
Text
id pubmed-8514779
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85147792021-10-15 Supplemental Dietary Selenohomolanthionine Improve Antioxidant Activity and Immune Function in Weaned Beagle Puppies Shao, Chunyan Zheng, Moufeng Yu, Ziwei Jiang, Sheng Zhou, Bin Song, Quanjiang Ma, Tianning Zhou, Yingshan Dong, Wanyu Li, Ding Gu, Yao Wang, Xiaodu Song, Houhui Front Vet Sci Veterinary Science The purpose of this study was to investigate the effects of dietary Selenohomolanthionine (SeHLan) on antioxidant status and immune response in canine parvovirus (CPV) vaccinated puppies. In this study, 30 weaned puppies were randomly divided into six groups: control group (–Se/–Vacc), immunization group (–Se/+Vacc), supplementation of sodium selenite group (SS/+Vacc, 0.35 mg/kg DM), low-dose SeHLan group (SeHLan-L/+Vacc, 0.35 mg/kg DM), mid-dose SeHLan group (SeHLan-M/+Vacc, 1.0 mg/kg DM), and high-dose SeHLan group (SeHLan-H/+Vacc, 2.0 mg/kg DM). The puppies were fed for 42 days and vaccinated with Vanguard Plus 5 on day 0 and day 21. Blood samples were collected on 7, 14, 21, 28, 35, 42 days post-immunization (PI) for determination of antioxidant indicators, lymphocyte proliferation index, serum cytokine concentration (IL-2, IL-4), canine polymorphonuclear neutrophils (PMN) phagocytic function, and the level of CPV antibody titers. The results showed that SeHLan supplementation raised the serum Se concentration and glutathione peroxidase (GSH-Px) activity in a dose-dependent manner (P < 0.05). It also increased the activity of serum superoxide dismutase (SOD) and decreased serum malondialdehyde (MDA) content, especially in SeHLan-M/+Vacc group (1.0 mg/kg DM) (P < 0.01). SeHLan supplementation significantly increased lymphocyte proliferation, IL-2, and IL-4 levels in canine serum, and enhanced phagocytosis of PMN in vaccinated puppies (P < 0.05). Moreover, SeHLan supplementation shortened the CPV antibody production time and increased the CPV antibody titers (P < 0.05). Of note, the beneficial effects of SeHLan were superior to those of SS. In conclusion, dietary SeHLan supplementation improved antioxidant activity, increased CPV antibody titers, and enhanced immune function in puppies after weaning. An appropriate dosage of SeHLan (1~2 mg/kg DM) may confer nutritional benefits in puppies. Frontiers Media S.A. 2021-09-30 /pmc/articles/PMC8514779/ /pubmed/34660766 http://dx.doi.org/10.3389/fvets.2021.728358 Text en Copyright © 2021 Shao, Zheng, Yu, Jiang, Zhou, Song, Ma, Zhou, Dong, Li, Gu, Wang and Song. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Shao, Chunyan
Zheng, Moufeng
Yu, Ziwei
Jiang, Sheng
Zhou, Bin
Song, Quanjiang
Ma, Tianning
Zhou, Yingshan
Dong, Wanyu
Li, Ding
Gu, Yao
Wang, Xiaodu
Song, Houhui
Supplemental Dietary Selenohomolanthionine Improve Antioxidant Activity and Immune Function in Weaned Beagle Puppies
title Supplemental Dietary Selenohomolanthionine Improve Antioxidant Activity and Immune Function in Weaned Beagle Puppies
title_full Supplemental Dietary Selenohomolanthionine Improve Antioxidant Activity and Immune Function in Weaned Beagle Puppies
title_fullStr Supplemental Dietary Selenohomolanthionine Improve Antioxidant Activity and Immune Function in Weaned Beagle Puppies
title_full_unstemmed Supplemental Dietary Selenohomolanthionine Improve Antioxidant Activity and Immune Function in Weaned Beagle Puppies
title_short Supplemental Dietary Selenohomolanthionine Improve Antioxidant Activity and Immune Function in Weaned Beagle Puppies
title_sort supplemental dietary selenohomolanthionine improve antioxidant activity and immune function in weaned beagle puppies
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8514779/
https://www.ncbi.nlm.nih.gov/pubmed/34660766
http://dx.doi.org/10.3389/fvets.2021.728358
work_keys_str_mv AT shaochunyan supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT zhengmoufeng supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT yuziwei supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT jiangsheng supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT zhoubin supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT songquanjiang supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT matianning supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT zhouyingshan supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT dongwanyu supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT liding supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT guyao supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT wangxiaodu supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies
AT songhouhui supplementaldietaryselenohomolanthionineimproveantioxidantactivityandimmunefunctioninweanedbeaglepuppies