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Modified ELISA for antibody avidity evaluation: The need for standardization

Antibody avidity is an important parameter to evaluate immune response, being useful to evaluate vaccine responses and helping to distinguish acute and latent infection. The antibody avidity can be measured by different methods, yet the most common is a modified ELISA. The utilization of commercial...

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Detalles Bibliográficos
Autores principales: Correa, Victor Araujo, Rodrigues, Thais Sousa, Portilho, Amanda Izeli, Trzewikoswki de Lima, Gabriela, De Gaspari, Elizabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chang Gung University 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515003/
https://www.ncbi.nlm.nih.gov/pubmed/34493482
http://dx.doi.org/10.1016/j.bj.2020.10.009
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author Correa, Victor Araujo
Rodrigues, Thais Sousa
Portilho, Amanda Izeli
Trzewikoswki de Lima, Gabriela
De Gaspari, Elizabeth
author_facet Correa, Victor Araujo
Rodrigues, Thais Sousa
Portilho, Amanda Izeli
Trzewikoswki de Lima, Gabriela
De Gaspari, Elizabeth
author_sort Correa, Victor Araujo
collection PubMed
description Antibody avidity is an important parameter to evaluate immune response, being useful to evaluate vaccine responses and helping to distinguish acute and latent infection. The antibody avidity can be measured by different methods, yet the most common is a modified ELISA. The utilization of commercial kits or in-house methods to evaluate antibody avidity have been adopted more and more, although the lack of standardization between different assays may generate a lot of variation in the process, making it hard to compare the results generated.
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spelling pubmed-85150032021-10-21 Modified ELISA for antibody avidity evaluation: The need for standardization Correa, Victor Araujo Rodrigues, Thais Sousa Portilho, Amanda Izeli Trzewikoswki de Lima, Gabriela De Gaspari, Elizabeth Biomed J Review Article Antibody avidity is an important parameter to evaluate immune response, being useful to evaluate vaccine responses and helping to distinguish acute and latent infection. The antibody avidity can be measured by different methods, yet the most common is a modified ELISA. The utilization of commercial kits or in-house methods to evaluate antibody avidity have been adopted more and more, although the lack of standardization between different assays may generate a lot of variation in the process, making it hard to compare the results generated. Chang Gung University 2021-08 2020-10-27 /pmc/articles/PMC8515003/ /pubmed/34493482 http://dx.doi.org/10.1016/j.bj.2020.10.009 Text en © 2020 Chang Gung University. Publishing services by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review Article
Correa, Victor Araujo
Rodrigues, Thais Sousa
Portilho, Amanda Izeli
Trzewikoswki de Lima, Gabriela
De Gaspari, Elizabeth
Modified ELISA for antibody avidity evaluation: The need for standardization
title Modified ELISA for antibody avidity evaluation: The need for standardization
title_full Modified ELISA for antibody avidity evaluation: The need for standardization
title_fullStr Modified ELISA for antibody avidity evaluation: The need for standardization
title_full_unstemmed Modified ELISA for antibody avidity evaluation: The need for standardization
title_short Modified ELISA for antibody avidity evaluation: The need for standardization
title_sort modified elisa for antibody avidity evaluation: the need for standardization
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515003/
https://www.ncbi.nlm.nih.gov/pubmed/34493482
http://dx.doi.org/10.1016/j.bj.2020.10.009
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