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Enhanced Susceptibility of ADAP-Deficient Mice to Listeria monocytogenes Infection Is Associated With an Altered Phagocyte Phenotype and Function

The adhesion and degranulation-promoting adaptor protein (ADAP) serves as a multifunctional scaffold and is involved in the formation of immune signaling complexes. To date, only limited data exist regarding the role of ADAP in pathogen-specific immunity during in vivo infection, and its contributio...

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Autores principales: Böning, Martha A. L., Parzmair, Gerald P., Jeron, Andreas, Düsedau, Henning P., Kershaw, Olivia, Xu, Baolin, Relja, Borna, Schlüter, Dirk, Dunay, Ildiko Rita, Reinhold, Annegret, Schraven, Burkhart, Bruder, Dunja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515145/
https://www.ncbi.nlm.nih.gov/pubmed/34659211
http://dx.doi.org/10.3389/fimmu.2021.724855
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author Böning, Martha A. L.
Parzmair, Gerald P.
Jeron, Andreas
Düsedau, Henning P.
Kershaw, Olivia
Xu, Baolin
Relja, Borna
Schlüter, Dirk
Dunay, Ildiko Rita
Reinhold, Annegret
Schraven, Burkhart
Bruder, Dunja
author_facet Böning, Martha A. L.
Parzmair, Gerald P.
Jeron, Andreas
Düsedau, Henning P.
Kershaw, Olivia
Xu, Baolin
Relja, Borna
Schlüter, Dirk
Dunay, Ildiko Rita
Reinhold, Annegret
Schraven, Burkhart
Bruder, Dunja
author_sort Böning, Martha A. L.
collection PubMed
description The adhesion and degranulation-promoting adaptor protein (ADAP) serves as a multifunctional scaffold and is involved in the formation of immune signaling complexes. To date, only limited data exist regarding the role of ADAP in pathogen-specific immunity during in vivo infection, and its contribution in phagocyte-mediated antibacterial immunity remains elusive. Here, we show that mice lacking ADAP (ADAPko) are highly susceptible to the infection with the intracellular pathogen Listeria monocytogenes (Lm) by showing enhanced immunopathology in infected tissues together with increased morbidity, mortality, and excessive infiltration of neutrophils and monocytes. Despite high phagocyte numbers in the spleen and liver, ADAPko mice only inefficiently controlled pathogen growth, hinting at a functional impairment of infection-primed phagocytes in the ADAP-deficient host. Flow cytometric analysis of hallmark pro-inflammatory mediators and unbiased whole genome transcriptional profiling of neutrophils and inflammatory monocytes uncovered broad molecular alterations in the inflammatory program in both phagocyte subsets following their activation in the ADAP-deficient host. Strikingly, ex vivo phagocytosis assay revealed impaired phagocytic capacity of neutrophils derived from Lm-infected ADAPko mice. Together, our data suggest that an alternative priming of phagocytes in ADAP-deficient mice during Lm infection induces marked alterations in the inflammatory profile of neutrophils and inflammatory monocytes that contribute to enhanced immunopathology while limiting their capacity to eliminate the pathogen and to prevent the fatal outcome of the infection.
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spelling pubmed-85151452021-10-15 Enhanced Susceptibility of ADAP-Deficient Mice to Listeria monocytogenes Infection Is Associated With an Altered Phagocyte Phenotype and Function Böning, Martha A. L. Parzmair, Gerald P. Jeron, Andreas Düsedau, Henning P. Kershaw, Olivia Xu, Baolin Relja, Borna Schlüter, Dirk Dunay, Ildiko Rita Reinhold, Annegret Schraven, Burkhart Bruder, Dunja Front Immunol Immunology The adhesion and degranulation-promoting adaptor protein (ADAP) serves as a multifunctional scaffold and is involved in the formation of immune signaling complexes. To date, only limited data exist regarding the role of ADAP in pathogen-specific immunity during in vivo infection, and its contribution in phagocyte-mediated antibacterial immunity remains elusive. Here, we show that mice lacking ADAP (ADAPko) are highly susceptible to the infection with the intracellular pathogen Listeria monocytogenes (Lm) by showing enhanced immunopathology in infected tissues together with increased morbidity, mortality, and excessive infiltration of neutrophils and monocytes. Despite high phagocyte numbers in the spleen and liver, ADAPko mice only inefficiently controlled pathogen growth, hinting at a functional impairment of infection-primed phagocytes in the ADAP-deficient host. Flow cytometric analysis of hallmark pro-inflammatory mediators and unbiased whole genome transcriptional profiling of neutrophils and inflammatory monocytes uncovered broad molecular alterations in the inflammatory program in both phagocyte subsets following their activation in the ADAP-deficient host. Strikingly, ex vivo phagocytosis assay revealed impaired phagocytic capacity of neutrophils derived from Lm-infected ADAPko mice. Together, our data suggest that an alternative priming of phagocytes in ADAP-deficient mice during Lm infection induces marked alterations in the inflammatory profile of neutrophils and inflammatory monocytes that contribute to enhanced immunopathology while limiting their capacity to eliminate the pathogen and to prevent the fatal outcome of the infection. Frontiers Media S.A. 2021-09-30 /pmc/articles/PMC8515145/ /pubmed/34659211 http://dx.doi.org/10.3389/fimmu.2021.724855 Text en Copyright © 2021 Böning, Parzmair, Jeron, Düsedau, Kershaw, Xu, Relja, Schlüter, Dunay, Reinhold, Schraven and Bruder https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Böning, Martha A. L.
Parzmair, Gerald P.
Jeron, Andreas
Düsedau, Henning P.
Kershaw, Olivia
Xu, Baolin
Relja, Borna
Schlüter, Dirk
Dunay, Ildiko Rita
Reinhold, Annegret
Schraven, Burkhart
Bruder, Dunja
Enhanced Susceptibility of ADAP-Deficient Mice to Listeria monocytogenes Infection Is Associated With an Altered Phagocyte Phenotype and Function
title Enhanced Susceptibility of ADAP-Deficient Mice to Listeria monocytogenes Infection Is Associated With an Altered Phagocyte Phenotype and Function
title_full Enhanced Susceptibility of ADAP-Deficient Mice to Listeria monocytogenes Infection Is Associated With an Altered Phagocyte Phenotype and Function
title_fullStr Enhanced Susceptibility of ADAP-Deficient Mice to Listeria monocytogenes Infection Is Associated With an Altered Phagocyte Phenotype and Function
title_full_unstemmed Enhanced Susceptibility of ADAP-Deficient Mice to Listeria monocytogenes Infection Is Associated With an Altered Phagocyte Phenotype and Function
title_short Enhanced Susceptibility of ADAP-Deficient Mice to Listeria monocytogenes Infection Is Associated With an Altered Phagocyte Phenotype and Function
title_sort enhanced susceptibility of adap-deficient mice to listeria monocytogenes infection is associated with an altered phagocyte phenotype and function
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515145/
https://www.ncbi.nlm.nih.gov/pubmed/34659211
http://dx.doi.org/10.3389/fimmu.2021.724855
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