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MicroRNA 142-5p promotes tumor growth in oral squamous cell carcinoma via the PI3K/AKT pathway by regulating PTEN

MicroRNAs (miRNAs) play an important role in carcinogenesis and cancer progression. The purpose of this study was to identify miRNAs associated with carcinoma function in OSCC and to investigate the potential role of the specific miRNAs. First, a comprehensive microarray analysis was performed, and...

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Autores principales: Iizumi, Seiichiro, Uchida, Fumihiko, Nagai, Hiroki, Takaoka, Shohei, Fukuzawa, Satoshi, Kanno, Naomi Ishibashi, Yamagata, Kenji, Tabuchi, Katsuhiko, Yanagawa, Toru, Bukawa, Hiroki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515248/
https://www.ncbi.nlm.nih.gov/pubmed/34693046
http://dx.doi.org/10.1016/j.heliyon.2021.e08086
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author Iizumi, Seiichiro
Uchida, Fumihiko
Nagai, Hiroki
Takaoka, Shohei
Fukuzawa, Satoshi
Kanno, Naomi Ishibashi
Yamagata, Kenji
Tabuchi, Katsuhiko
Yanagawa, Toru
Bukawa, Hiroki
author_facet Iizumi, Seiichiro
Uchida, Fumihiko
Nagai, Hiroki
Takaoka, Shohei
Fukuzawa, Satoshi
Kanno, Naomi Ishibashi
Yamagata, Kenji
Tabuchi, Katsuhiko
Yanagawa, Toru
Bukawa, Hiroki
author_sort Iizumi, Seiichiro
collection PubMed
description MicroRNAs (miRNAs) play an important role in carcinogenesis and cancer progression. The purpose of this study was to identify miRNAs associated with carcinoma function in OSCC and to investigate the potential role of the specific miRNAs. First, a comprehensive microarray analysis was performed, and miR-142-5p was identified as a candidate miRNA involved in OSCC. miR-142-5p has been reported to show high expression levels in cancer patients and to be involved in tumor growth and metastasis. However, the expression and function of miR-142-5p in oral squamous cell carcinoma (OSCC) are not fully characterized. We evaluated miR-142-5p expression in both OSCC-derived cell lines and primary OSCC tissues and performed functional analysis of miR-142-5p in OSCC-derived cell lines using mimics and inhibitors. miR-142-5p expression was up-regulated in OSCC tissues and OSCC cell lines. Overexpression of miR-142-5p significantly promoted the proliferation and invasion of OSCC cells. Bioinformatics analysis was performed using TargetScan to predict potential target sites that match the seed region of miR-142-5p. Phosphatase and tensin homolog deleted on chromo-some 10 (PTEN) was identified as a potential target and selected for further analysis. PTEN expression levels were down-regulated and AKT expression levels were up-regulated in miR-142-5p-overexpressing cells. We have shown that miR-142-5p targets the PTEN gene and is involved in cancer progression. Our results suggest that miR-142-5p is involved in the progression of OSCC by controlling the phosphatidylinositol 3-kinase (PI3K)/AKT pathway by targeting the PTEN gene. Our findings suggest that miR-142-5p may be a new target for the treatment of OSCC.
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spelling pubmed-85152482021-10-21 MicroRNA 142-5p promotes tumor growth in oral squamous cell carcinoma via the PI3K/AKT pathway by regulating PTEN Iizumi, Seiichiro Uchida, Fumihiko Nagai, Hiroki Takaoka, Shohei Fukuzawa, Satoshi Kanno, Naomi Ishibashi Yamagata, Kenji Tabuchi, Katsuhiko Yanagawa, Toru Bukawa, Hiroki Heliyon Research Article MicroRNAs (miRNAs) play an important role in carcinogenesis and cancer progression. The purpose of this study was to identify miRNAs associated with carcinoma function in OSCC and to investigate the potential role of the specific miRNAs. First, a comprehensive microarray analysis was performed, and miR-142-5p was identified as a candidate miRNA involved in OSCC. miR-142-5p has been reported to show high expression levels in cancer patients and to be involved in tumor growth and metastasis. However, the expression and function of miR-142-5p in oral squamous cell carcinoma (OSCC) are not fully characterized. We evaluated miR-142-5p expression in both OSCC-derived cell lines and primary OSCC tissues and performed functional analysis of miR-142-5p in OSCC-derived cell lines using mimics and inhibitors. miR-142-5p expression was up-regulated in OSCC tissues and OSCC cell lines. Overexpression of miR-142-5p significantly promoted the proliferation and invasion of OSCC cells. Bioinformatics analysis was performed using TargetScan to predict potential target sites that match the seed region of miR-142-5p. Phosphatase and tensin homolog deleted on chromo-some 10 (PTEN) was identified as a potential target and selected for further analysis. PTEN expression levels were down-regulated and AKT expression levels were up-regulated in miR-142-5p-overexpressing cells. We have shown that miR-142-5p targets the PTEN gene and is involved in cancer progression. Our results suggest that miR-142-5p is involved in the progression of OSCC by controlling the phosphatidylinositol 3-kinase (PI3K)/AKT pathway by targeting the PTEN gene. Our findings suggest that miR-142-5p may be a new target for the treatment of OSCC. Elsevier 2021-09-30 /pmc/articles/PMC8515248/ /pubmed/34693046 http://dx.doi.org/10.1016/j.heliyon.2021.e08086 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Iizumi, Seiichiro
Uchida, Fumihiko
Nagai, Hiroki
Takaoka, Shohei
Fukuzawa, Satoshi
Kanno, Naomi Ishibashi
Yamagata, Kenji
Tabuchi, Katsuhiko
Yanagawa, Toru
Bukawa, Hiroki
MicroRNA 142-5p promotes tumor growth in oral squamous cell carcinoma via the PI3K/AKT pathway by regulating PTEN
title MicroRNA 142-5p promotes tumor growth in oral squamous cell carcinoma via the PI3K/AKT pathway by regulating PTEN
title_full MicroRNA 142-5p promotes tumor growth in oral squamous cell carcinoma via the PI3K/AKT pathway by regulating PTEN
title_fullStr MicroRNA 142-5p promotes tumor growth in oral squamous cell carcinoma via the PI3K/AKT pathway by regulating PTEN
title_full_unstemmed MicroRNA 142-5p promotes tumor growth in oral squamous cell carcinoma via the PI3K/AKT pathway by regulating PTEN
title_short MicroRNA 142-5p promotes tumor growth in oral squamous cell carcinoma via the PI3K/AKT pathway by regulating PTEN
title_sort microrna 142-5p promotes tumor growth in oral squamous cell carcinoma via the pi3k/akt pathway by regulating pten
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515248/
https://www.ncbi.nlm.nih.gov/pubmed/34693046
http://dx.doi.org/10.1016/j.heliyon.2021.e08086
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