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Effects of Caffeine Consumption on Autologous Full-Thickness Skin Graft Healing in an Animal Model
Background There exists contradictory evidence that states both the beneficial and deleterious effects of caffeine on wound healing. The general population might unknowingly consume caffeine that negatively affects wound healing. The main objective of this study is to investigate the effect of daily...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Thieme Medical and Scientific Publishers Pvt. Ltd.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515418/ https://www.ncbi.nlm.nih.gov/pubmed/34667517 http://dx.doi.org/10.1055/s-0041-1734573 |
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author | Supit, Tommy Susilaningsih, Neni Prasetyo, Awal |
author_facet | Supit, Tommy Susilaningsih, Neni Prasetyo, Awal |
author_sort | Supit, Tommy |
collection | PubMed |
description | Background There exists contradictory evidence that states both the beneficial and deleterious effects of caffeine on wound healing. The general population might unknowingly consume caffeine that negatively affects wound healing. The main objective of this study is to investigate the effect of daily caffeine consumption on wound healing, specifically full-thickness skin graft (FTSG). Methods Forty Sprague–Dawley rats were randomized into four groups of equal size: control-dose (CD), low-dose (LD), medium-dose (MD), and high-dose (HD) caffeine groups. After autologous FTSG, all subjects in the intervention group were given daily pure caffeine gavage. The FTSG was explanted 7 days posttransplant. The graft viability, secondary contraction, and adherence were evaluated macroscopically, while fibroblast and collagen deposition was analyzed microscopically with hematoxylin eosin stain. Results The least graft viability (72.8 ± 20.7%, clinical wound assessment scale [CWAS] 2.4), highest secondary contraction (11.4 ± 10.5%), and fibroblast count (331.8 ± 88.6 cells/5 high power fields) were observed in the MD group. More collagen synthesis was observed in subjects who consumed caffeine. The level of secondary contraction, fibroblast count as well as graft viability and collagen synthesis were positively correlated. Conclusions Daily consumption of caffeine impairs graft viability when given in medium dose and increases collagen synthesis, irrespective of dosage. This study was in experimental rats; the results are not directly translatable to humans. |
format | Online Article Text |
id | pubmed-8515418 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85154182021-10-18 Effects of Caffeine Consumption on Autologous Full-Thickness Skin Graft Healing in an Animal Model Supit, Tommy Susilaningsih, Neni Prasetyo, Awal Indian J Plast Surg Background There exists contradictory evidence that states both the beneficial and deleterious effects of caffeine on wound healing. The general population might unknowingly consume caffeine that negatively affects wound healing. The main objective of this study is to investigate the effect of daily caffeine consumption on wound healing, specifically full-thickness skin graft (FTSG). Methods Forty Sprague–Dawley rats were randomized into four groups of equal size: control-dose (CD), low-dose (LD), medium-dose (MD), and high-dose (HD) caffeine groups. After autologous FTSG, all subjects in the intervention group were given daily pure caffeine gavage. The FTSG was explanted 7 days posttransplant. The graft viability, secondary contraction, and adherence were evaluated macroscopically, while fibroblast and collagen deposition was analyzed microscopically with hematoxylin eosin stain. Results The least graft viability (72.8 ± 20.7%, clinical wound assessment scale [CWAS] 2.4), highest secondary contraction (11.4 ± 10.5%), and fibroblast count (331.8 ± 88.6 cells/5 high power fields) were observed in the MD group. More collagen synthesis was observed in subjects who consumed caffeine. The level of secondary contraction, fibroblast count as well as graft viability and collagen synthesis were positively correlated. Conclusions Daily consumption of caffeine impairs graft viability when given in medium dose and increases collagen synthesis, irrespective of dosage. This study was in experimental rats; the results are not directly translatable to humans. Thieme Medical and Scientific Publishers Pvt. Ltd. 2021-09-16 /pmc/articles/PMC8515418/ /pubmed/34667517 http://dx.doi.org/10.1055/s-0041-1734573 Text en Association of Plastic Surgeons of India. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/). https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited. |
spellingShingle | Supit, Tommy Susilaningsih, Neni Prasetyo, Awal Effects of Caffeine Consumption on Autologous Full-Thickness Skin Graft Healing in an Animal Model |
title | Effects of Caffeine Consumption on Autologous Full-Thickness Skin Graft Healing in an Animal Model |
title_full | Effects of Caffeine Consumption on Autologous Full-Thickness Skin Graft Healing in an Animal Model |
title_fullStr | Effects of Caffeine Consumption on Autologous Full-Thickness Skin Graft Healing in an Animal Model |
title_full_unstemmed | Effects of Caffeine Consumption on Autologous Full-Thickness Skin Graft Healing in an Animal Model |
title_short | Effects of Caffeine Consumption on Autologous Full-Thickness Skin Graft Healing in an Animal Model |
title_sort | effects of caffeine consumption on autologous full-thickness skin graft healing in an animal model |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515418/ https://www.ncbi.nlm.nih.gov/pubmed/34667517 http://dx.doi.org/10.1055/s-0041-1734573 |
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