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Effects and mechanisms of mUCMSCs on ovarian structure and function in naturally ageing C57 mice

BACKGROUND: The ovaries are the core reproductive organs in women and are critical for maintaining normal reproductive function and endocrine system stability. An ageing C57 mouse model was used to evaluate the efficacy and mechanism of mouse umbilical cord mesenchymal stem cells (mUCMSCs) and to ex...

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Autores principales: Pan, Xing-Hua, Zhang, Xue-Juan, Yao, Xiang, Tian, Ni-Ni, Yang, Zai-Ling, Wang, Kai, Zhu, Xiang-Qing, Zhao, Jing, He, Jie, Cai, Xue-Min, Pang, Rong-Qing, Ruan, Guang-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515706/
https://www.ncbi.nlm.nih.gov/pubmed/34645513
http://dx.doi.org/10.1186/s13048-021-00854-5
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author Pan, Xing-Hua
Zhang, Xue-Juan
Yao, Xiang
Tian, Ni-Ni
Yang, Zai-Ling
Wang, Kai
Zhu, Xiang-Qing
Zhao, Jing
He, Jie
Cai, Xue-Min
Pang, Rong-Qing
Ruan, Guang-Ping
author_facet Pan, Xing-Hua
Zhang, Xue-Juan
Yao, Xiang
Tian, Ni-Ni
Yang, Zai-Ling
Wang, Kai
Zhu, Xiang-Qing
Zhao, Jing
He, Jie
Cai, Xue-Min
Pang, Rong-Qing
Ruan, Guang-Ping
author_sort Pan, Xing-Hua
collection PubMed
description BACKGROUND: The ovaries are the core reproductive organs in women and are critical for maintaining normal reproductive function and endocrine system stability. An ageing C57 mouse model was used to evaluate the efficacy and mechanism of mouse umbilical cord mesenchymal stem cells (mUCMSCs) and to explore the mechanism by which mUCMSCs promote the antioxidant repair of mouse granulosa cells (mGCs). RESULTS: Eighteen-month-old C57 mice were randomly divided into a model group and a treatment group. At the same time, 2-month-old C57 mice were established as a young group (15 mice per group). The mice in the treatment group were injected via the tail vein with GFP-labelled mUCMSCs. The ovarian volume in ageing C57 mice was decreased, and there were no follicles at any stage. After mUCMSC transplantation, the mouse ovaries increased in size, follicles at various stages were observed in the cortex, and the antral follicle counts increased. The serum E2, AMH, and INH-B levels of mice in the treatment group were significantly higher than those of mice in the model control group (P < 0.05). mUCMSCs downregulated the expression of the autophagy-related gene LC3b and the apoptosis-related genes Bax and Caspase-3, upregulated the expression of SOD2 and the peroxidase gene PRDX IV, and reduced apoptosis rates and reactive oxygen species (ROS) levels in granulosa cells. CONCLUSIONS: mUCMSCs play roles in promoting the repair of ageing ovaries by regulating immunity, anti-inflammatory responses and the PI3K-Akt signalling pathway. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13048-021-00854-5.
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spelling pubmed-85157062021-10-20 Effects and mechanisms of mUCMSCs on ovarian structure and function in naturally ageing C57 mice Pan, Xing-Hua Zhang, Xue-Juan Yao, Xiang Tian, Ni-Ni Yang, Zai-Ling Wang, Kai Zhu, Xiang-Qing Zhao, Jing He, Jie Cai, Xue-Min Pang, Rong-Qing Ruan, Guang-Ping J Ovarian Res Research BACKGROUND: The ovaries are the core reproductive organs in women and are critical for maintaining normal reproductive function and endocrine system stability. An ageing C57 mouse model was used to evaluate the efficacy and mechanism of mouse umbilical cord mesenchymal stem cells (mUCMSCs) and to explore the mechanism by which mUCMSCs promote the antioxidant repair of mouse granulosa cells (mGCs). RESULTS: Eighteen-month-old C57 mice were randomly divided into a model group and a treatment group. At the same time, 2-month-old C57 mice were established as a young group (15 mice per group). The mice in the treatment group were injected via the tail vein with GFP-labelled mUCMSCs. The ovarian volume in ageing C57 mice was decreased, and there were no follicles at any stage. After mUCMSC transplantation, the mouse ovaries increased in size, follicles at various stages were observed in the cortex, and the antral follicle counts increased. The serum E2, AMH, and INH-B levels of mice in the treatment group were significantly higher than those of mice in the model control group (P < 0.05). mUCMSCs downregulated the expression of the autophagy-related gene LC3b and the apoptosis-related genes Bax and Caspase-3, upregulated the expression of SOD2 and the peroxidase gene PRDX IV, and reduced apoptosis rates and reactive oxygen species (ROS) levels in granulosa cells. CONCLUSIONS: mUCMSCs play roles in promoting the repair of ageing ovaries by regulating immunity, anti-inflammatory responses and the PI3K-Akt signalling pathway. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13048-021-00854-5. BioMed Central 2021-10-13 /pmc/articles/PMC8515706/ /pubmed/34645513 http://dx.doi.org/10.1186/s13048-021-00854-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Pan, Xing-Hua
Zhang, Xue-Juan
Yao, Xiang
Tian, Ni-Ni
Yang, Zai-Ling
Wang, Kai
Zhu, Xiang-Qing
Zhao, Jing
He, Jie
Cai, Xue-Min
Pang, Rong-Qing
Ruan, Guang-Ping
Effects and mechanisms of mUCMSCs on ovarian structure and function in naturally ageing C57 mice
title Effects and mechanisms of mUCMSCs on ovarian structure and function in naturally ageing C57 mice
title_full Effects and mechanisms of mUCMSCs on ovarian structure and function in naturally ageing C57 mice
title_fullStr Effects and mechanisms of mUCMSCs on ovarian structure and function in naturally ageing C57 mice
title_full_unstemmed Effects and mechanisms of mUCMSCs on ovarian structure and function in naturally ageing C57 mice
title_short Effects and mechanisms of mUCMSCs on ovarian structure and function in naturally ageing C57 mice
title_sort effects and mechanisms of mucmscs on ovarian structure and function in naturally ageing c57 mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515706/
https://www.ncbi.nlm.nih.gov/pubmed/34645513
http://dx.doi.org/10.1186/s13048-021-00854-5
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