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Increased systemic RNA oxidative damage and diagnostic value of RNA oxidative metabolites during Shigella flexneri-induced intestinal infection

BACKGROUND: Shigella flexneri (S. flexneri) is a major pathogen causing acute intestinal infection, but the systematic oxidative damage incurred during the course of infection has not been investigated. AIM: To investigate the incurred systemic RNA oxidative damage and the diagnostic value of RNA ox...

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Autores principales: Nie, Jing-Jing, Pian, Ya-Ya, Hu, Ji-Hong, Fan, Guo-Qing, Zeng, Lv-Tao, Ouyang, Qiu-Geng, Gao, Zhen-Xiang, Liu, Zhen, Wang, Chen-Chen, Liu, Qian, Cai, Jian-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515791/
https://www.ncbi.nlm.nih.gov/pubmed/34712030
http://dx.doi.org/10.3748/wjg.v27.i37.6248
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author Nie, Jing-Jing
Pian, Ya-Ya
Hu, Ji-Hong
Fan, Guo-Qing
Zeng, Lv-Tao
Ouyang, Qiu-Geng
Gao, Zhen-Xiang
Liu, Zhen
Wang, Chen-Chen
Liu, Qian
Cai, Jian-Ping
author_facet Nie, Jing-Jing
Pian, Ya-Ya
Hu, Ji-Hong
Fan, Guo-Qing
Zeng, Lv-Tao
Ouyang, Qiu-Geng
Gao, Zhen-Xiang
Liu, Zhen
Wang, Chen-Chen
Liu, Qian
Cai, Jian-Ping
author_sort Nie, Jing-Jing
collection PubMed
description BACKGROUND: Shigella flexneri (S. flexneri) is a major pathogen causing acute intestinal infection, but the systematic oxidative damage incurred during the course of infection has not been investigated. AIM: To investigate the incurred systemic RNA oxidative damage and the diagnostic value of RNA oxidative metabolites during S. flexneri-induced intestinal infection. METHODS: In this study, a Sprague-Dawley rat model of acute intestinal infection was established by oral gavage with S. flexneri strains. The changes in white blood cells (WBCs) and cytokine levels in blood and the inflammatory response in the colon were investigated. We also detected the RNA and DNA oxidation in urine and tissues. RESULTS: S. flexneri infection induced an increase in WBCs, C-reactive protein, interleukin (IL)-6, IL-10, IL-1β, IL-4, IL-17a, IL-10, and tumor necrosis factor α (TNF-α) in blood. Of note, a significant increase in urinary 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn), an important marker of total RNA oxidation, was detected after intestinal infection (P = 0.03). The urinary 8-oxo-Gsn level returned to the baseline level after recovery from infection. In addition, the results of a correlation analysis showed that urinary 8-oxo-Gsn was positively correlated with the WBC count and the cytokines IL-6, TNF-α, IL-10, IL-1β, and IL-17α. Further detection of the oxidation in different tissues showed that S. flexneri infection induced RNA oxidative damage in the colon, ileum, liver, spleen, and brain. CONCLUSION: Acute infection induced by S. flexneri causes increased RNA oxidative damage in various tissues (liver, spleen, and brain) and an increase of 8-oxo-Gsn, a urinary metabolite. Urinary 8-oxo-Gsn may be useful as a biomarker for evaluating the severity and prognosis of infection.
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spelling pubmed-85157912021-10-27 Increased systemic RNA oxidative damage and diagnostic value of RNA oxidative metabolites during Shigella flexneri-induced intestinal infection Nie, Jing-Jing Pian, Ya-Ya Hu, Ji-Hong Fan, Guo-Qing Zeng, Lv-Tao Ouyang, Qiu-Geng Gao, Zhen-Xiang Liu, Zhen Wang, Chen-Chen Liu, Qian Cai, Jian-Ping World J Gastroenterol Basic Study BACKGROUND: Shigella flexneri (S. flexneri) is a major pathogen causing acute intestinal infection, but the systematic oxidative damage incurred during the course of infection has not been investigated. AIM: To investigate the incurred systemic RNA oxidative damage and the diagnostic value of RNA oxidative metabolites during S. flexneri-induced intestinal infection. METHODS: In this study, a Sprague-Dawley rat model of acute intestinal infection was established by oral gavage with S. flexneri strains. The changes in white blood cells (WBCs) and cytokine levels in blood and the inflammatory response in the colon were investigated. We also detected the RNA and DNA oxidation in urine and tissues. RESULTS: S. flexneri infection induced an increase in WBCs, C-reactive protein, interleukin (IL)-6, IL-10, IL-1β, IL-4, IL-17a, IL-10, and tumor necrosis factor α (TNF-α) in blood. Of note, a significant increase in urinary 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn), an important marker of total RNA oxidation, was detected after intestinal infection (P = 0.03). The urinary 8-oxo-Gsn level returned to the baseline level after recovery from infection. In addition, the results of a correlation analysis showed that urinary 8-oxo-Gsn was positively correlated with the WBC count and the cytokines IL-6, TNF-α, IL-10, IL-1β, and IL-17α. Further detection of the oxidation in different tissues showed that S. flexneri infection induced RNA oxidative damage in the colon, ileum, liver, spleen, and brain. CONCLUSION: Acute infection induced by S. flexneri causes increased RNA oxidative damage in various tissues (liver, spleen, and brain) and an increase of 8-oxo-Gsn, a urinary metabolite. Urinary 8-oxo-Gsn may be useful as a biomarker for evaluating the severity and prognosis of infection. Baishideng Publishing Group Inc 2021-10-07 2021-10-07 /pmc/articles/PMC8515791/ /pubmed/34712030 http://dx.doi.org/10.3748/wjg.v27.i37.6248 Text en ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
spellingShingle Basic Study
Nie, Jing-Jing
Pian, Ya-Ya
Hu, Ji-Hong
Fan, Guo-Qing
Zeng, Lv-Tao
Ouyang, Qiu-Geng
Gao, Zhen-Xiang
Liu, Zhen
Wang, Chen-Chen
Liu, Qian
Cai, Jian-Ping
Increased systemic RNA oxidative damage and diagnostic value of RNA oxidative metabolites during Shigella flexneri-induced intestinal infection
title Increased systemic RNA oxidative damage and diagnostic value of RNA oxidative metabolites during Shigella flexneri-induced intestinal infection
title_full Increased systemic RNA oxidative damage and diagnostic value of RNA oxidative metabolites during Shigella flexneri-induced intestinal infection
title_fullStr Increased systemic RNA oxidative damage and diagnostic value of RNA oxidative metabolites during Shigella flexneri-induced intestinal infection
title_full_unstemmed Increased systemic RNA oxidative damage and diagnostic value of RNA oxidative metabolites during Shigella flexneri-induced intestinal infection
title_short Increased systemic RNA oxidative damage and diagnostic value of RNA oxidative metabolites during Shigella flexneri-induced intestinal infection
title_sort increased systemic rna oxidative damage and diagnostic value of rna oxidative metabolites during shigella flexneri-induced intestinal infection
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515791/
https://www.ncbi.nlm.nih.gov/pubmed/34712030
http://dx.doi.org/10.3748/wjg.v27.i37.6248
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