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Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus

Cases of pox-like lesions in horses and donkeys have been associated with poxviruses belonging to different genera of the family Poxviridae. These include the orthopoxviruses vaccinia virus (VACV), horsepoxvirus (HPXV) and cowpoxvirus (CPXV), as well as a potentially novel parapoxvirus and molluscum...

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Autores principales: Ehmann, Rosina, Brandes, K., Antwerpen, M., Walter, M., v. Schlippenbach, K., Stegmaier, E., Essbauer, S., Bugert, J., Teifke, J. P., Meyer, H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515872/
https://www.ncbi.nlm.nih.gov/pubmed/31922947
http://dx.doi.org/10.1099/jgv.0.001357
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author Ehmann, Rosina
Brandes, K.
Antwerpen, M.
Walter, M.
v. Schlippenbach, K.
Stegmaier, E.
Essbauer, S.
Bugert, J.
Teifke, J. P.
Meyer, H.
author_facet Ehmann, Rosina
Brandes, K.
Antwerpen, M.
Walter, M.
v. Schlippenbach, K.
Stegmaier, E.
Essbauer, S.
Bugert, J.
Teifke, J. P.
Meyer, H.
author_sort Ehmann, Rosina
collection PubMed
description Cases of pox-like lesions in horses and donkeys have been associated with poxviruses belonging to different genera of the family Poxviridae. These include the orthopoxviruses vaccinia virus (VACV), horsepoxvirus (HPXV) and cowpoxvirus (CPXV), as well as a potentially novel parapoxvirus and molluscum contagiosum virus (MOCV). However, with the exception of VACV, HPXV and CPXV, the genomic characterization of the causative agents remains largely elusive with only single short genome fragments available. Here we present the first full-length genome sequence of an equine molluscum contagiosum-like virus (EMCLV) directly determined from skin biopsies of a horse with generalized papular dermatitis. Histopathological analysis of the lesions revealed severe epidermal hyperplasia with numerous eosinophilic inclusion bodies within keratinocytes. Virions were detected in the lesions in embedded tissue by transmission electron microscopy. The genome sequence determined by next- and third-generation sequencing comprises 166 843 nt with inverted terminal repeats (ITRs) of 3473 nt. Overall, 20 of the predicted 159 ORFs have no equivalents in other poxviruses. Intriguingly, two of these ORFs were identified to encode homologues of mammalian proteins involved in immune signalling pathways, namely secreted and transmembrane protein 1 (SECTM1) and insulin growth factor-like family receptor 1 (IGFLR1), that were not described in any virus family so far. Phylogenetic analysis with all relevant representatives of the Poxviridae suggests that EMCLV should be nominated as a new species within the genus Molluscipoxvirus.
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spelling pubmed-85158722021-10-15 Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus Ehmann, Rosina Brandes, K. Antwerpen, M. Walter, M. v. Schlippenbach, K. Stegmaier, E. Essbauer, S. Bugert, J. Teifke, J. P. Meyer, H. J Gen Virol Research Article Cases of pox-like lesions in horses and donkeys have been associated with poxviruses belonging to different genera of the family Poxviridae. These include the orthopoxviruses vaccinia virus (VACV), horsepoxvirus (HPXV) and cowpoxvirus (CPXV), as well as a potentially novel parapoxvirus and molluscum contagiosum virus (MOCV). However, with the exception of VACV, HPXV and CPXV, the genomic characterization of the causative agents remains largely elusive with only single short genome fragments available. Here we present the first full-length genome sequence of an equine molluscum contagiosum-like virus (EMCLV) directly determined from skin biopsies of a horse with generalized papular dermatitis. Histopathological analysis of the lesions revealed severe epidermal hyperplasia with numerous eosinophilic inclusion bodies within keratinocytes. Virions were detected in the lesions in embedded tissue by transmission electron microscopy. The genome sequence determined by next- and third-generation sequencing comprises 166 843 nt with inverted terminal repeats (ITRs) of 3473 nt. Overall, 20 of the predicted 159 ORFs have no equivalents in other poxviruses. Intriguingly, two of these ORFs were identified to encode homologues of mammalian proteins involved in immune signalling pathways, namely secreted and transmembrane protein 1 (SECTM1) and insulin growth factor-like family receptor 1 (IGFLR1), that were not described in any virus family so far. Phylogenetic analysis with all relevant representatives of the Poxviridae suggests that EMCLV should be nominated as a new species within the genus Molluscipoxvirus. Microbiology Society 2020-01-10 /pmc/articles/PMC8515872/ /pubmed/31922947 http://dx.doi.org/10.1099/jgv.0.001357 Text en © 2021 Not applicable https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
spellingShingle Research Article
Ehmann, Rosina
Brandes, K.
Antwerpen, M.
Walter, M.
v. Schlippenbach, K.
Stegmaier, E.
Essbauer, S.
Bugert, J.
Teifke, J. P.
Meyer, H.
Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus
title Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus
title_full Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus
title_fullStr Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus
title_full_unstemmed Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus
title_short Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus
title_sort molecular and genomic characterization of a novel equine molluscum contagiosum-like virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515872/
https://www.ncbi.nlm.nih.gov/pubmed/31922947
http://dx.doi.org/10.1099/jgv.0.001357
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