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Oral Microbiome Alterations and SARS-CoV-2 Saliva Viral Load in Patients with COVID-19

Bacterial-viral interactions in saliva have been associated with morbidity and mortality for respiratory viruses such as influenza and SARS-CoV. However, such transkingdom relationships during SARS-CoV-2 infection are currently unknown. Here, we aimed to elucidate the relationship between saliva mic...

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Detalles Bibliográficos
Autores principales: Miller, Emily Happy, Annavajhala, Medini K., Chong, Alexander M., Park, Heekuk, Nobel, Yael R., Soroush, Ali, Blackett, John W., Krigel, Anna, Phipps, Meaghan M., Freedberg, Daniel E., Zucker, Jason, Sano, Ellen D., Uhlemann, Anne-Catrin, Abrams, Julian A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515944/
https://www.ncbi.nlm.nih.gov/pubmed/34643448
http://dx.doi.org/10.1128/Spectrum.00055-21
Descripción
Sumario:Bacterial-viral interactions in saliva have been associated with morbidity and mortality for respiratory viruses such as influenza and SARS-CoV. However, such transkingdom relationships during SARS-CoV-2 infection are currently unknown. Here, we aimed to elucidate the relationship between saliva microbiota and SARS-CoV-2 in a cohort of newly hospitalized COVID-19 patients and controls. We used 16S rRNA sequencing to compare microbiome diversity and taxonomic composition between COVID-19 patients (n = 53) and controls (n = 59) and based on saliva SARS-CoV-2 viral load as measured using reverse transcription PCR (RT-PCR). The saliva microbiome did not differ markedly between COVID-19 patients and controls. However, we identified significant differential abundance of numerous taxa based on saliva SARS-CoV-2 viral load, including multiple species within Streptococcus and Prevotella. IMPORTANCE Alterations to the saliva microbiome based on SARS-CoV-2 viral load indicate potential biologically relevant bacterial-viral relationships which may affect clinical outcomes in COVID-19 disease.