Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord

To fill the prevailing gap in methodology for whole spinal cord (SC) analysis, we have (1) designed scaffolds (SpineRacks) that facilitate efficient and ordered cryo-sectioning of the entire SC in a single block, (2) constructed a 3D reference atlas of adult mouse SC, and (3) developed software (Spi...

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Detalles Bibliográficos
Autores principales: Fiederling, Felix, Hammond, Luke A., Ng, David, Mason, Carol, Dodd, Jane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516137/
https://www.ncbi.nlm.nih.gov/pubmed/34661190
http://dx.doi.org/10.1016/j.crmeth.2021.100074
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author Fiederling, Felix
Hammond, Luke A.
Ng, David
Mason, Carol
Dodd, Jane
author_facet Fiederling, Felix
Hammond, Luke A.
Ng, David
Mason, Carol
Dodd, Jane
author_sort Fiederling, Felix
collection PubMed
description To fill the prevailing gap in methodology for whole spinal cord (SC) analysis, we have (1) designed scaffolds (SpineRacks) that facilitate efficient and ordered cryo-sectioning of the entire SC in a single block, (2) constructed a 3D reference atlas of adult mouse SC, and (3) developed software (SpinalJ) to register images of sections and for standardized analysis of cells and projections in atlas space. We have verified mapping accuracies for known neurons and demonstrated the usefulness of this platform to reveal unknown neuronal distributions. Together, these tools provide high-throughput analyses of whole mouse SC and enable direct comparison of 3D spatial information between animals and studies.
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spelling pubmed-85161372021-10-14 Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord Fiederling, Felix Hammond, Luke A. Ng, David Mason, Carol Dodd, Jane Cell Rep Methods Article To fill the prevailing gap in methodology for whole spinal cord (SC) analysis, we have (1) designed scaffolds (SpineRacks) that facilitate efficient and ordered cryo-sectioning of the entire SC in a single block, (2) constructed a 3D reference atlas of adult mouse SC, and (3) developed software (SpinalJ) to register images of sections and for standardized analysis of cells and projections in atlas space. We have verified mapping accuracies for known neurons and demonstrated the usefulness of this platform to reveal unknown neuronal distributions. Together, these tools provide high-throughput analyses of whole mouse SC and enable direct comparison of 3D spatial information between animals and studies. Elsevier 2021-09-15 /pmc/articles/PMC8516137/ /pubmed/34661190 http://dx.doi.org/10.1016/j.crmeth.2021.100074 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Fiederling, Felix
Hammond, Luke A.
Ng, David
Mason, Carol
Dodd, Jane
Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord
title Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord
title_full Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord
title_fullStr Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord
title_full_unstemmed Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord
title_short Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord
title_sort tools for efficient analysis of neurons in a 3d reference atlas of whole mouse spinal cord
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516137/
https://www.ncbi.nlm.nih.gov/pubmed/34661190
http://dx.doi.org/10.1016/j.crmeth.2021.100074
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