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Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach

The frequency of arboviral disease epidemics is increasing and vector control remains the primary mechanism to limit arboviral transmission. Container inhabiting mosquitoes such as Aedes albopictus and Aedes aegypti are the primary vectors of dengue, chikungunya, and Zika viruses. Current vector con...

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Autores principales: Kancharlapalli, Sri Jyosthsna, Crabtree, Cameron J., Surowiec, Kaz, Longing, Scott D., L. Brelsfoard, Corey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516248/
https://www.ncbi.nlm.nih.gov/pubmed/34648501
http://dx.doi.org/10.1371/journal.pntd.0009824
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author Kancharlapalli, Sri Jyosthsna
Crabtree, Cameron J.
Surowiec, Kaz
Longing, Scott D.
L. Brelsfoard, Corey
author_facet Kancharlapalli, Sri Jyosthsna
Crabtree, Cameron J.
Surowiec, Kaz
Longing, Scott D.
L. Brelsfoard, Corey
author_sort Kancharlapalli, Sri Jyosthsna
collection PubMed
description The frequency of arboviral disease epidemics is increasing and vector control remains the primary mechanism to limit arboviral transmission. Container inhabiting mosquitoes such as Aedes albopictus and Aedes aegypti are the primary vectors of dengue, chikungunya, and Zika viruses. Current vector control methods for these species are often ineffective, suggesting the need for novel control approaches. A proposed novel approach is autodissemination of insect growth regulators (IGRs). The advantage of autodissemination approaches is small amounts of active ingredients compared to traditional insecticide applications are used to impact mosquito populations. While the direct targeting of cryptic locations via autodissemination seems like a significant advantage over large scale applications of insecticides, this approach could actually affect nontarget organisms by delivering these highly potent long lasting growth inhibitors such as pyriproxyfen (PPF) to the exact locations that other beneficial insects visit, such as a nectar source. Here we tested the hypothesis that PPF treated male Ae. albopictus will contaminate nectar sources, which results in the indirect transfer of PPF to European honey bees (Apis mellifera). We performed bioassays, fluorescent imaging, and mass spectrometry on insect and artificial nectar source materials to examine for intra- and interspecific transfer of PPF. Data suggests there is direct transfer of PPF from Ae. albopictus PPF treated males and indirect transfer of PPF to A. mellifera from artificial nectar sources. In addition, we show a reduction in fecundity in Ae. albopictus and Drosophila melanogaster when exposed to sublethal doses of PPF. The observed transfer of PPF to A. mellifera suggests the need for further investigation of autodissemination approaches in a more field like setting to examine for risks to insect pollinators.
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spelling pubmed-85162482021-10-15 Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach Kancharlapalli, Sri Jyosthsna Crabtree, Cameron J. Surowiec, Kaz Longing, Scott D. L. Brelsfoard, Corey PLoS Negl Trop Dis Research Article The frequency of arboviral disease epidemics is increasing and vector control remains the primary mechanism to limit arboviral transmission. Container inhabiting mosquitoes such as Aedes albopictus and Aedes aegypti are the primary vectors of dengue, chikungunya, and Zika viruses. Current vector control methods for these species are often ineffective, suggesting the need for novel control approaches. A proposed novel approach is autodissemination of insect growth regulators (IGRs). The advantage of autodissemination approaches is small amounts of active ingredients compared to traditional insecticide applications are used to impact mosquito populations. While the direct targeting of cryptic locations via autodissemination seems like a significant advantage over large scale applications of insecticides, this approach could actually affect nontarget organisms by delivering these highly potent long lasting growth inhibitors such as pyriproxyfen (PPF) to the exact locations that other beneficial insects visit, such as a nectar source. Here we tested the hypothesis that PPF treated male Ae. albopictus will contaminate nectar sources, which results in the indirect transfer of PPF to European honey bees (Apis mellifera). We performed bioassays, fluorescent imaging, and mass spectrometry on insect and artificial nectar source materials to examine for intra- and interspecific transfer of PPF. Data suggests there is direct transfer of PPF from Ae. albopictus PPF treated males and indirect transfer of PPF to A. mellifera from artificial nectar sources. In addition, we show a reduction in fecundity in Ae. albopictus and Drosophila melanogaster when exposed to sublethal doses of PPF. The observed transfer of PPF to A. mellifera suggests the need for further investigation of autodissemination approaches in a more field like setting to examine for risks to insect pollinators. Public Library of Science 2021-10-14 /pmc/articles/PMC8516248/ /pubmed/34648501 http://dx.doi.org/10.1371/journal.pntd.0009824 Text en © 2021 Kancharlapalli et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kancharlapalli, Sri Jyosthsna
Crabtree, Cameron J.
Surowiec, Kaz
Longing, Scott D.
L. Brelsfoard, Corey
Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach
title Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach
title_full Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach
title_fullStr Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach
title_full_unstemmed Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach
title_short Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach
title_sort indirect transfer of pyriproxyfen to european honeybees via an autodissemination approach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516248/
https://www.ncbi.nlm.nih.gov/pubmed/34648501
http://dx.doi.org/10.1371/journal.pntd.0009824
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