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The structure of the cereal leaf beetle (Oulema melanopus) microbiome depends on the insect’s developmental stage, host plant, and origin

Cereal leaf beetle (CLB, Oulema melanopus, Coleoptera, Chrysomelidae) is a serious agricultural pest that causes considerable damages to agricultural production. The aim of this study was to characterize the bacterial communities associated with larvae and imagoes of CLB collected from various cerea...

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Autores principales: Wielkopolan, Beata, Krawczyk, Krzysztof, Szabelska-Beręsewicz, Alicja, Obrępalska-Stęplowska, Aleksandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516949/
https://www.ncbi.nlm.nih.gov/pubmed/34650106
http://dx.doi.org/10.1038/s41598-021-99411-9
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author Wielkopolan, Beata
Krawczyk, Krzysztof
Szabelska-Beręsewicz, Alicja
Obrępalska-Stęplowska, Aleksandra
author_facet Wielkopolan, Beata
Krawczyk, Krzysztof
Szabelska-Beręsewicz, Alicja
Obrępalska-Stęplowska, Aleksandra
author_sort Wielkopolan, Beata
collection PubMed
description Cereal leaf beetle (CLB, Oulema melanopus, Coleoptera, Chrysomelidae) is a serious agricultural pest that causes considerable damages to agricultural production. The aim of this study was to characterize the bacterial communities associated with larvae and imagoes of CLB collected from various cereal host species and locations. The bacterial profile was characterized by 16S rRNA gene sequencing at the V3-V4 hypervariable region. Using taxonomy-based analysis, the bacterial community of CLB containing 16 phyla, 26 classes, 49 orders, 78 families, 94 genera, and 63 species of bacteria was identified. The abundance of Wolbachia, Rickettsia, and Lactococcus genus was significantly higher in CLB imagoes than in larvae. Statistical analysis confirmed that the bacterial community of the larvae is more diverse in comparison to imagoes and that insects collected from spring barley and wheat are characterized by a much higher biodiversity level of bacterial genera and species than insects collected from other cereals. Obtained results indicated that the developmental stage, the host plant, and the insect’s sampling location affected the CLB’s microbiome. Additionally, the CLB core microbiome was determined. It consists of 2 genera (Wolbachia and Rickettsia) shared by at least 90% tested CLB insects, regardless of the variables analysed.
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spelling pubmed-85169492021-10-15 The structure of the cereal leaf beetle (Oulema melanopus) microbiome depends on the insect’s developmental stage, host plant, and origin Wielkopolan, Beata Krawczyk, Krzysztof Szabelska-Beręsewicz, Alicja Obrępalska-Stęplowska, Aleksandra Sci Rep Article Cereal leaf beetle (CLB, Oulema melanopus, Coleoptera, Chrysomelidae) is a serious agricultural pest that causes considerable damages to agricultural production. The aim of this study was to characterize the bacterial communities associated with larvae and imagoes of CLB collected from various cereal host species and locations. The bacterial profile was characterized by 16S rRNA gene sequencing at the V3-V4 hypervariable region. Using taxonomy-based analysis, the bacterial community of CLB containing 16 phyla, 26 classes, 49 orders, 78 families, 94 genera, and 63 species of bacteria was identified. The abundance of Wolbachia, Rickettsia, and Lactococcus genus was significantly higher in CLB imagoes than in larvae. Statistical analysis confirmed that the bacterial community of the larvae is more diverse in comparison to imagoes and that insects collected from spring barley and wheat are characterized by a much higher biodiversity level of bacterial genera and species than insects collected from other cereals. Obtained results indicated that the developmental stage, the host plant, and the insect’s sampling location affected the CLB’s microbiome. Additionally, the CLB core microbiome was determined. It consists of 2 genera (Wolbachia and Rickettsia) shared by at least 90% tested CLB insects, regardless of the variables analysed. Nature Publishing Group UK 2021-10-14 /pmc/articles/PMC8516949/ /pubmed/34650106 http://dx.doi.org/10.1038/s41598-021-99411-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wielkopolan, Beata
Krawczyk, Krzysztof
Szabelska-Beręsewicz, Alicja
Obrępalska-Stęplowska, Aleksandra
The structure of the cereal leaf beetle (Oulema melanopus) microbiome depends on the insect’s developmental stage, host plant, and origin
title The structure of the cereal leaf beetle (Oulema melanopus) microbiome depends on the insect’s developmental stage, host plant, and origin
title_full The structure of the cereal leaf beetle (Oulema melanopus) microbiome depends on the insect’s developmental stage, host plant, and origin
title_fullStr The structure of the cereal leaf beetle (Oulema melanopus) microbiome depends on the insect’s developmental stage, host plant, and origin
title_full_unstemmed The structure of the cereal leaf beetle (Oulema melanopus) microbiome depends on the insect’s developmental stage, host plant, and origin
title_short The structure of the cereal leaf beetle (Oulema melanopus) microbiome depends on the insect’s developmental stage, host plant, and origin
title_sort structure of the cereal leaf beetle (oulema melanopus) microbiome depends on the insect’s developmental stage, host plant, and origin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516949/
https://www.ncbi.nlm.nih.gov/pubmed/34650106
http://dx.doi.org/10.1038/s41598-021-99411-9
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