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Transcriptomics of chicken cecal tonsils and intestine after infection with low pathogenic avian influenza virus H9N2
Influenza viruses cause severe respiratory infections in humans and birds, triggering global health concerns and economic burden. Influenza infection is a dynamic process involving complex biological host responses. The objective of this study was to illustrate global biological processes in ileum a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517014/ https://www.ncbi.nlm.nih.gov/pubmed/34650121 http://dx.doi.org/10.1038/s41598-021-99182-3 |
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author | Alqazlan, Nadiyah Emam, Mehdi Nagy, Éva Bridle, Byram Sargolzaei, Mehdi Sharif, Shayan |
author_facet | Alqazlan, Nadiyah Emam, Mehdi Nagy, Éva Bridle, Byram Sargolzaei, Mehdi Sharif, Shayan |
author_sort | Alqazlan, Nadiyah |
collection | PubMed |
description | Influenza viruses cause severe respiratory infections in humans and birds, triggering global health concerns and economic burden. Influenza infection is a dynamic process involving complex biological host responses. The objective of this study was to illustrate global biological processes in ileum and cecal tonsils at early time points after chickens were infected with low pathogenic avian influenza virus (LPAIV) H9N2 through transcriptome analysis. Total RNA isolated from ileum and cecal tonsils of non-infected and infected layers at 12-, 24- and 72-h post-infection (hpi) was used for mRNA sequencing analyses to characterize differentially expressed genes and overrepresented pathways. Statistical analysis highlighted transcriptomic signatures significantly occurring 24 and 72 hpi, but not earlier at 12 hpi. Interferon (IFN)-inducible and IFN-stimulated gene (ISG) expression was increased, followed by continued expression of various heat-shock proteins (HSP), including HSP60, HSP70, HSP90 and HSP110. Some upregulated genes involved in innate antiviral responses included DDX60, MX1, RSAD2 and CMPK2. The ISG15 antiviral mechanism pathway was highly enriched in ileum and cecal tonsils at 24 hpi. Overall, most affected pathways were related to interferon production and the heat-shock response. Research on these candidate genes and pathways is warranted to decipher underlying mechanisms of immunity against LPAIV in chickens. |
format | Online Article Text |
id | pubmed-8517014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85170142021-10-15 Transcriptomics of chicken cecal tonsils and intestine after infection with low pathogenic avian influenza virus H9N2 Alqazlan, Nadiyah Emam, Mehdi Nagy, Éva Bridle, Byram Sargolzaei, Mehdi Sharif, Shayan Sci Rep Article Influenza viruses cause severe respiratory infections in humans and birds, triggering global health concerns and economic burden. Influenza infection is a dynamic process involving complex biological host responses. The objective of this study was to illustrate global biological processes in ileum and cecal tonsils at early time points after chickens were infected with low pathogenic avian influenza virus (LPAIV) H9N2 through transcriptome analysis. Total RNA isolated from ileum and cecal tonsils of non-infected and infected layers at 12-, 24- and 72-h post-infection (hpi) was used for mRNA sequencing analyses to characterize differentially expressed genes and overrepresented pathways. Statistical analysis highlighted transcriptomic signatures significantly occurring 24 and 72 hpi, but not earlier at 12 hpi. Interferon (IFN)-inducible and IFN-stimulated gene (ISG) expression was increased, followed by continued expression of various heat-shock proteins (HSP), including HSP60, HSP70, HSP90 and HSP110. Some upregulated genes involved in innate antiviral responses included DDX60, MX1, RSAD2 and CMPK2. The ISG15 antiviral mechanism pathway was highly enriched in ileum and cecal tonsils at 24 hpi. Overall, most affected pathways were related to interferon production and the heat-shock response. Research on these candidate genes and pathways is warranted to decipher underlying mechanisms of immunity against LPAIV in chickens. Nature Publishing Group UK 2021-10-14 /pmc/articles/PMC8517014/ /pubmed/34650121 http://dx.doi.org/10.1038/s41598-021-99182-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Alqazlan, Nadiyah Emam, Mehdi Nagy, Éva Bridle, Byram Sargolzaei, Mehdi Sharif, Shayan Transcriptomics of chicken cecal tonsils and intestine after infection with low pathogenic avian influenza virus H9N2 |
title | Transcriptomics of chicken cecal tonsils and intestine after infection with low pathogenic avian influenza virus H9N2 |
title_full | Transcriptomics of chicken cecal tonsils and intestine after infection with low pathogenic avian influenza virus H9N2 |
title_fullStr | Transcriptomics of chicken cecal tonsils and intestine after infection with low pathogenic avian influenza virus H9N2 |
title_full_unstemmed | Transcriptomics of chicken cecal tonsils and intestine after infection with low pathogenic avian influenza virus H9N2 |
title_short | Transcriptomics of chicken cecal tonsils and intestine after infection with low pathogenic avian influenza virus H9N2 |
title_sort | transcriptomics of chicken cecal tonsils and intestine after infection with low pathogenic avian influenza virus h9n2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517014/ https://www.ncbi.nlm.nih.gov/pubmed/34650121 http://dx.doi.org/10.1038/s41598-021-99182-3 |
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