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Coral-Derived Endophytic Fungal Product, Butyrolactone-I, Alleviates Lps Induced Intestinal Epithelial Cell Inflammatory Response Through TLR4/NF-κB and MAPK Signaling Pathways: An in vitro and in vivo Studies

Herein, we assessed the anti-inflammatory and intestinal barrier protective effects of butyrolactone-I (BTL-1), derived from the coral-derived endophytic fungus (Aspergillus terreus), using the LPS-induced IPEC-J2 inflammation model and the DSS-induced IBD model in mice. In IPEC-J2 cells, pretreatme...

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Autores principales: Chen, Shengwei, Zhang, Yi, Niu, Xueting, Mohyuddin, Sahar Ghulam, Wen, Jiayin, Bao, Minglong, Yu, Tianyue, Wu, Lianyun, Hu, Canyin, Yong, Yanhong, Liu, Xiaoxi, Abd El-Aty, A. M., Ju, Xianghong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517189/
https://www.ncbi.nlm.nih.gov/pubmed/34660669
http://dx.doi.org/10.3389/fnut.2021.748118
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author Chen, Shengwei
Zhang, Yi
Niu, Xueting
Mohyuddin, Sahar Ghulam
Wen, Jiayin
Bao, Minglong
Yu, Tianyue
Wu, Lianyun
Hu, Canyin
Yong, Yanhong
Liu, Xiaoxi
Abd El-Aty, A. M.
Ju, Xianghong
author_facet Chen, Shengwei
Zhang, Yi
Niu, Xueting
Mohyuddin, Sahar Ghulam
Wen, Jiayin
Bao, Minglong
Yu, Tianyue
Wu, Lianyun
Hu, Canyin
Yong, Yanhong
Liu, Xiaoxi
Abd El-Aty, A. M.
Ju, Xianghong
author_sort Chen, Shengwei
collection PubMed
description Herein, we assessed the anti-inflammatory and intestinal barrier protective effects of butyrolactone-I (BTL-1), derived from the coral-derived endophytic fungus (Aspergillus terreus), using the LPS-induced IPEC-J2 inflammation model and the DSS-induced IBD model in mice. In IPEC-J2 cells, pretreatment with BTL-I significantly inhibited TLR4/NF-κB signaling pathway and JNK phosphorylation, resulting in the decrease of IL-1β and IL-6 expression. Interestingly, BTL-1 pretreatment activated the phosphorylation of ERK and P38, which significantly enhanced the expression of TNF-α. Meanwhile, BTL-1 pretreatment upregulated tight junction protein expression (ZO-1, occludin, and claudin-1) and maintained intestinal barrier and intestinal permeability integrity. In mice, BTL-1 significantly alleviated the intestinal inflammatory response induced by DSS, inhibited TLR4/NF-κB signaling pathway, and MAPK signaling pathway, thus reducing the production of IL-1, IL-6, and TNF-α. Further, the expression of tight junction proteins (ZO-1, occludin, and claudin-1) was upregulated in BTL-1 administrated mice. Therefore, it has been suggested that butyrolactone-I alleviates inflammatory responses in LPS-stimulated IPEC-J2 and DSS-induced murine colitis by TLR4/NF-κB and MAPK signal pathway. Thereby, BTL-1 might potentially be used as an ocean drug to prevent intestinal bowel disease.
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spelling pubmed-85171892021-10-16 Coral-Derived Endophytic Fungal Product, Butyrolactone-I, Alleviates Lps Induced Intestinal Epithelial Cell Inflammatory Response Through TLR4/NF-κB and MAPK Signaling Pathways: An in vitro and in vivo Studies Chen, Shengwei Zhang, Yi Niu, Xueting Mohyuddin, Sahar Ghulam Wen, Jiayin Bao, Minglong Yu, Tianyue Wu, Lianyun Hu, Canyin Yong, Yanhong Liu, Xiaoxi Abd El-Aty, A. M. Ju, Xianghong Front Nutr Nutrition Herein, we assessed the anti-inflammatory and intestinal barrier protective effects of butyrolactone-I (BTL-1), derived from the coral-derived endophytic fungus (Aspergillus terreus), using the LPS-induced IPEC-J2 inflammation model and the DSS-induced IBD model in mice. In IPEC-J2 cells, pretreatment with BTL-I significantly inhibited TLR4/NF-κB signaling pathway and JNK phosphorylation, resulting in the decrease of IL-1β and IL-6 expression. Interestingly, BTL-1 pretreatment activated the phosphorylation of ERK and P38, which significantly enhanced the expression of TNF-α. Meanwhile, BTL-1 pretreatment upregulated tight junction protein expression (ZO-1, occludin, and claudin-1) and maintained intestinal barrier and intestinal permeability integrity. In mice, BTL-1 significantly alleviated the intestinal inflammatory response induced by DSS, inhibited TLR4/NF-κB signaling pathway, and MAPK signaling pathway, thus reducing the production of IL-1, IL-6, and TNF-α. Further, the expression of tight junction proteins (ZO-1, occludin, and claudin-1) was upregulated in BTL-1 administrated mice. Therefore, it has been suggested that butyrolactone-I alleviates inflammatory responses in LPS-stimulated IPEC-J2 and DSS-induced murine colitis by TLR4/NF-κB and MAPK signal pathway. Thereby, BTL-1 might potentially be used as an ocean drug to prevent intestinal bowel disease. Frontiers Media S.A. 2021-10-01 /pmc/articles/PMC8517189/ /pubmed/34660669 http://dx.doi.org/10.3389/fnut.2021.748118 Text en Copyright © 2021 Chen, Zhang, Niu, Mohyuddin, Wen, Bao, Yu, Wu, Hu, Yong, Liu, Abd El-Aty and Ju. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Chen, Shengwei
Zhang, Yi
Niu, Xueting
Mohyuddin, Sahar Ghulam
Wen, Jiayin
Bao, Minglong
Yu, Tianyue
Wu, Lianyun
Hu, Canyin
Yong, Yanhong
Liu, Xiaoxi
Abd El-Aty, A. M.
Ju, Xianghong
Coral-Derived Endophytic Fungal Product, Butyrolactone-I, Alleviates Lps Induced Intestinal Epithelial Cell Inflammatory Response Through TLR4/NF-κB and MAPK Signaling Pathways: An in vitro and in vivo Studies
title Coral-Derived Endophytic Fungal Product, Butyrolactone-I, Alleviates Lps Induced Intestinal Epithelial Cell Inflammatory Response Through TLR4/NF-κB and MAPK Signaling Pathways: An in vitro and in vivo Studies
title_full Coral-Derived Endophytic Fungal Product, Butyrolactone-I, Alleviates Lps Induced Intestinal Epithelial Cell Inflammatory Response Through TLR4/NF-κB and MAPK Signaling Pathways: An in vitro and in vivo Studies
title_fullStr Coral-Derived Endophytic Fungal Product, Butyrolactone-I, Alleviates Lps Induced Intestinal Epithelial Cell Inflammatory Response Through TLR4/NF-κB and MAPK Signaling Pathways: An in vitro and in vivo Studies
title_full_unstemmed Coral-Derived Endophytic Fungal Product, Butyrolactone-I, Alleviates Lps Induced Intestinal Epithelial Cell Inflammatory Response Through TLR4/NF-κB and MAPK Signaling Pathways: An in vitro and in vivo Studies
title_short Coral-Derived Endophytic Fungal Product, Butyrolactone-I, Alleviates Lps Induced Intestinal Epithelial Cell Inflammatory Response Through TLR4/NF-κB and MAPK Signaling Pathways: An in vitro and in vivo Studies
title_sort coral-derived endophytic fungal product, butyrolactone-i, alleviates lps induced intestinal epithelial cell inflammatory response through tlr4/nf-κb and mapk signaling pathways: an in vitro and in vivo studies
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517189/
https://www.ncbi.nlm.nih.gov/pubmed/34660669
http://dx.doi.org/10.3389/fnut.2021.748118
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