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Interactome analysis reveals endocytosis and membrane recycling of EpCAM during differentiation of embryonic stem cells and carcinoma cells

Transmembrane epithelial cell adhesion molecule (EpCAM) is expressed in epithelia, carcinoma, teratoma, and embryonic stem cells (ESCs). EpCAM displays spatiotemporal patterning during embryogenesis, tissue morphogenesis, cell differentiation, and epithelial-to-mesenchymal transition (EMT) in carcin...

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Autores principales: Pan, Min, Kohlbauer, Vera, Blancke Soares, Alexandra, Schinke, Henrik, Huang, Yuanchi, Kranz, Gisela, Quadt, Tanja, Hachmeister, Matthias, Gires, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517208/
https://www.ncbi.nlm.nih.gov/pubmed/34693227
http://dx.doi.org/10.1016/j.isci.2021.103179
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author Pan, Min
Kohlbauer, Vera
Blancke Soares, Alexandra
Schinke, Henrik
Huang, Yuanchi
Kranz, Gisela
Quadt, Tanja
Hachmeister, Matthias
Gires, Olivier
author_facet Pan, Min
Kohlbauer, Vera
Blancke Soares, Alexandra
Schinke, Henrik
Huang, Yuanchi
Kranz, Gisela
Quadt, Tanja
Hachmeister, Matthias
Gires, Olivier
author_sort Pan, Min
collection PubMed
description Transmembrane epithelial cell adhesion molecule (EpCAM) is expressed in epithelia, carcinoma, teratoma, and embryonic stem cells (ESCs). EpCAM displays spatiotemporal patterning during embryogenesis, tissue morphogenesis, cell differentiation, and epithelial-to-mesenchymal transition (EMT) in carcinomas. Potential interactors of EpCAM were identified in murine F9 teratoma cells using a stable isotope labeling with amino acids in cell culture-based proteomic approach (n = 77, enrichment factor >3, p value ≤ 0.05). Kyoto Encyclopedia of Genes and Genomes and gene ontology terms revealed interactions with regulators of endosomal trafficking and membrane recycling, which were further validated for Rab5, Rab7, and Rab11. Endocytosis and membrane recycling of EpCAM were confirmed in mF9 cells, E14TG2α ESC, and Kyse30 carcinoma cells. Reduction of EpCAM during mesodermal differentiation and TGFβ-induced EMT correlated with enhanced endocytosis and block or reduction of recycling in ESCs and esophageal carcinoma cells. Hence, endocytosis and membrane recycling are means of regulation of EpCAM protein levels during differentiation of ESC and EMT induction in carcinoma cells.
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spelling pubmed-85172082021-10-21 Interactome analysis reveals endocytosis and membrane recycling of EpCAM during differentiation of embryonic stem cells and carcinoma cells Pan, Min Kohlbauer, Vera Blancke Soares, Alexandra Schinke, Henrik Huang, Yuanchi Kranz, Gisela Quadt, Tanja Hachmeister, Matthias Gires, Olivier iScience Article Transmembrane epithelial cell adhesion molecule (EpCAM) is expressed in epithelia, carcinoma, teratoma, and embryonic stem cells (ESCs). EpCAM displays spatiotemporal patterning during embryogenesis, tissue morphogenesis, cell differentiation, and epithelial-to-mesenchymal transition (EMT) in carcinomas. Potential interactors of EpCAM were identified in murine F9 teratoma cells using a stable isotope labeling with amino acids in cell culture-based proteomic approach (n = 77, enrichment factor >3, p value ≤ 0.05). Kyoto Encyclopedia of Genes and Genomes and gene ontology terms revealed interactions with regulators of endosomal trafficking and membrane recycling, which were further validated for Rab5, Rab7, and Rab11. Endocytosis and membrane recycling of EpCAM were confirmed in mF9 cells, E14TG2α ESC, and Kyse30 carcinoma cells. Reduction of EpCAM during mesodermal differentiation and TGFβ-induced EMT correlated with enhanced endocytosis and block or reduction of recycling in ESCs and esophageal carcinoma cells. Hence, endocytosis and membrane recycling are means of regulation of EpCAM protein levels during differentiation of ESC and EMT induction in carcinoma cells. Elsevier 2021-09-27 /pmc/articles/PMC8517208/ /pubmed/34693227 http://dx.doi.org/10.1016/j.isci.2021.103179 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pan, Min
Kohlbauer, Vera
Blancke Soares, Alexandra
Schinke, Henrik
Huang, Yuanchi
Kranz, Gisela
Quadt, Tanja
Hachmeister, Matthias
Gires, Olivier
Interactome analysis reveals endocytosis and membrane recycling of EpCAM during differentiation of embryonic stem cells and carcinoma cells
title Interactome analysis reveals endocytosis and membrane recycling of EpCAM during differentiation of embryonic stem cells and carcinoma cells
title_full Interactome analysis reveals endocytosis and membrane recycling of EpCAM during differentiation of embryonic stem cells and carcinoma cells
title_fullStr Interactome analysis reveals endocytosis and membrane recycling of EpCAM during differentiation of embryonic stem cells and carcinoma cells
title_full_unstemmed Interactome analysis reveals endocytosis and membrane recycling of EpCAM during differentiation of embryonic stem cells and carcinoma cells
title_short Interactome analysis reveals endocytosis and membrane recycling of EpCAM during differentiation of embryonic stem cells and carcinoma cells
title_sort interactome analysis reveals endocytosis and membrane recycling of epcam during differentiation of embryonic stem cells and carcinoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517208/
https://www.ncbi.nlm.nih.gov/pubmed/34693227
http://dx.doi.org/10.1016/j.isci.2021.103179
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