Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution

Zebrafish have been found to be a premier model organism in biological and regeneration research. However, the comprehensive cell compositions and molecular dynamics during tissue regeneration in zebrafish remain poorly understood. Here, we utilized Microwell-seq to analyze more than 250,000 single...

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Autores principales: Jiang, Mengmeng, Xiao, Yanyu, E, Weigao, Ma, Lifeng, Wang, Jingjing, Chen, Haide, Gao, Ce, Liao, Yuan, Guo, Qile, Peng, Jinrong, Han, Xiaoping, Guo, Guoji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517238/
https://www.ncbi.nlm.nih.gov/pubmed/34660600
http://dx.doi.org/10.3389/fcell.2021.743421
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author Jiang, Mengmeng
Xiao, Yanyu
E, Weigao
Ma, Lifeng
Wang, Jingjing
Chen, Haide
Gao, Ce
Liao, Yuan
Guo, Qile
Peng, Jinrong
Han, Xiaoping
Guo, Guoji
author_facet Jiang, Mengmeng
Xiao, Yanyu
E, Weigao
Ma, Lifeng
Wang, Jingjing
Chen, Haide
Gao, Ce
Liao, Yuan
Guo, Qile
Peng, Jinrong
Han, Xiaoping
Guo, Guoji
author_sort Jiang, Mengmeng
collection PubMed
description Zebrafish have been found to be a premier model organism in biological and regeneration research. However, the comprehensive cell compositions and molecular dynamics during tissue regeneration in zebrafish remain poorly understood. Here, we utilized Microwell-seq to analyze more than 250,000 single cells covering major zebrafish cell types and constructed a systematic zebrafish cell landscape. We revealed single-cell compositions for 18 zebrafish tissue types covering both embryo and adult stages. Single-cell mapping of caudal fin regeneration revealed a unique characteristic of blastema population and key genetic regulation involved in zebrafish tissue repair. Overall, our single-cell datasets demonstrate the utility of zebrafish cell landscape resources in various fields of biological research.
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spelling pubmed-85172382021-10-16 Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution Jiang, Mengmeng Xiao, Yanyu E, Weigao Ma, Lifeng Wang, Jingjing Chen, Haide Gao, Ce Liao, Yuan Guo, Qile Peng, Jinrong Han, Xiaoping Guo, Guoji Front Cell Dev Biol Cell and Developmental Biology Zebrafish have been found to be a premier model organism in biological and regeneration research. However, the comprehensive cell compositions and molecular dynamics during tissue regeneration in zebrafish remain poorly understood. Here, we utilized Microwell-seq to analyze more than 250,000 single cells covering major zebrafish cell types and constructed a systematic zebrafish cell landscape. We revealed single-cell compositions for 18 zebrafish tissue types covering both embryo and adult stages. Single-cell mapping of caudal fin regeneration revealed a unique characteristic of blastema population and key genetic regulation involved in zebrafish tissue repair. Overall, our single-cell datasets demonstrate the utility of zebrafish cell landscape resources in various fields of biological research. Frontiers Media S.A. 2021-10-01 /pmc/articles/PMC8517238/ /pubmed/34660600 http://dx.doi.org/10.3389/fcell.2021.743421 Text en Copyright © 2021 Jiang, Xiao, E, Ma, Wang, Chen, Gao, Liao, Guo, Peng, Han and Guo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Jiang, Mengmeng
Xiao, Yanyu
E, Weigao
Ma, Lifeng
Wang, Jingjing
Chen, Haide
Gao, Ce
Liao, Yuan
Guo, Qile
Peng, Jinrong
Han, Xiaoping
Guo, Guoji
Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution
title Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution
title_full Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution
title_fullStr Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution
title_full_unstemmed Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution
title_short Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution
title_sort characterization of the zebrafish cell landscape at single-cell resolution
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517238/
https://www.ncbi.nlm.nih.gov/pubmed/34660600
http://dx.doi.org/10.3389/fcell.2021.743421
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