Protocols for isolation and characterization of mouse placental hemogenic endothelial cells

The murine mid-gestational placenta has been identified as a hematopoietic site during embryonic development. Here, we describe a protocol for isolation and characterization of the hemogenic endothelial (HE) cells from mouse placenta. We also describe techniques for dissection of placental tissues a...

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Detalles Bibliográficos
Autores principales: Liang, Guixian, Huang, Baofeng, Wang, Fengchao, Liu, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517604/
https://www.ncbi.nlm.nih.gov/pubmed/34693362
http://dx.doi.org/10.1016/j.xpro.2021.100884
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author Liang, Guixian
Huang, Baofeng
Wang, Fengchao
Liu, Feng
author_facet Liang, Guixian
Huang, Baofeng
Wang, Fengchao
Liu, Feng
author_sort Liang, Guixian
collection PubMed
description The murine mid-gestational placenta has been identified as a hematopoietic site during embryonic development. Here, we describe a protocol for isolation and characterization of the hemogenic endothelial (HE) cells from mouse placenta. We also describe techniques for dissection of placental tissues and for the optimization of tissue digestion and antibody conjugation conditions to identify HE cells via fluorescence-activated cell sorting. For details on the usage and application of this protocol, please refer to Liang et al. (2021).
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spelling pubmed-85176042021-10-21 Protocols for isolation and characterization of mouse placental hemogenic endothelial cells Liang, Guixian Huang, Baofeng Wang, Fengchao Liu, Feng STAR Protoc Protocol The murine mid-gestational placenta has been identified as a hematopoietic site during embryonic development. Here, we describe a protocol for isolation and characterization of the hemogenic endothelial (HE) cells from mouse placenta. We also describe techniques for dissection of placental tissues and for the optimization of tissue digestion and antibody conjugation conditions to identify HE cells via fluorescence-activated cell sorting. For details on the usage and application of this protocol, please refer to Liang et al. (2021). Elsevier 2021-10-11 /pmc/articles/PMC8517604/ /pubmed/34693362 http://dx.doi.org/10.1016/j.xpro.2021.100884 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Liang, Guixian
Huang, Baofeng
Wang, Fengchao
Liu, Feng
Protocols for isolation and characterization of mouse placental hemogenic endothelial cells
title Protocols for isolation and characterization of mouse placental hemogenic endothelial cells
title_full Protocols for isolation and characterization of mouse placental hemogenic endothelial cells
title_fullStr Protocols for isolation and characterization of mouse placental hemogenic endothelial cells
title_full_unstemmed Protocols for isolation and characterization of mouse placental hemogenic endothelial cells
title_short Protocols for isolation and characterization of mouse placental hemogenic endothelial cells
title_sort protocols for isolation and characterization of mouse placental hemogenic endothelial cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517604/
https://www.ncbi.nlm.nih.gov/pubmed/34693362
http://dx.doi.org/10.1016/j.xpro.2021.100884
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