Cargando…

Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil

BACKGROUND: Secondary salinized soil in greenhouses often contains excess nitrate. Inoculation of Bacillus megaterium NCT-2 with nitrate assimilation ability represents an attractive approach for soil remediation. However, the effects of NCT-2 on the structure and function of soil microbial communit...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Bin, Chu, Shaohua, Liu, Xiaorui, Zhang, Dan, Chai, Xiaotong, Yang, Xijia, Zhi, Yuee, Chi, Yaowei, Zhou, Pei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519178/
https://www.ncbi.nlm.nih.gov/pubmed/34721987
http://dx.doi.org/10.7717/peerj.12309
_version_ 1784584396866584576
author Wang, Bin
Chu, Shaohua
Liu, Xiaorui
Zhang, Dan
Chai, Xiaotong
Yang, Xijia
Zhi, Yuee
Chi, Yaowei
Zhou, Pei
author_facet Wang, Bin
Chu, Shaohua
Liu, Xiaorui
Zhang, Dan
Chai, Xiaotong
Yang, Xijia
Zhi, Yuee
Chi, Yaowei
Zhou, Pei
author_sort Wang, Bin
collection PubMed
description BACKGROUND: Secondary salinized soil in greenhouses often contains excess nitrate. Inoculation of Bacillus megaterium NCT-2 with nitrate assimilation ability represents an attractive approach for soil remediation. However, the effects of NCT-2 on the structure and function of soil microbial communities have not been explored. METHODS: Greenhouse experiments were carried out to investigate changes in soil properties, Brassica chinensis L. growth, bacterial, and fungal community structure and function in response to NCT-2 inoculation. RESULTS: The NCT-2 inoculant significantly reduced the nitrate content in B. chinensis and inhibited the rebound of soil nitrate in the later stage. The shifts of bacterial community structure and function by NCT-2 was negligible, and a greater disturbance of soil fungal community structure and function was observed, for example the strong inhibitory effect on ectomycorrhizal fungi. These results indicated that the NCT-2 inoculant likely achieved the remediation effect in secondary salinized soil by shifting fungal community. The present findings add to the current understanding of microbial interactions in response to bacterial inoculation and can be of great significance for the application of NCT-2 inoculants in secondary salinized soil remediation.
format Online
Article
Text
id pubmed-8519178
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-85191782021-10-28 Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil Wang, Bin Chu, Shaohua Liu, Xiaorui Zhang, Dan Chai, Xiaotong Yang, Xijia Zhi, Yuee Chi, Yaowei Zhou, Pei PeerJ Agricultural Science BACKGROUND: Secondary salinized soil in greenhouses often contains excess nitrate. Inoculation of Bacillus megaterium NCT-2 with nitrate assimilation ability represents an attractive approach for soil remediation. However, the effects of NCT-2 on the structure and function of soil microbial communities have not been explored. METHODS: Greenhouse experiments were carried out to investigate changes in soil properties, Brassica chinensis L. growth, bacterial, and fungal community structure and function in response to NCT-2 inoculation. RESULTS: The NCT-2 inoculant significantly reduced the nitrate content in B. chinensis and inhibited the rebound of soil nitrate in the later stage. The shifts of bacterial community structure and function by NCT-2 was negligible, and a greater disturbance of soil fungal community structure and function was observed, for example the strong inhibitory effect on ectomycorrhizal fungi. These results indicated that the NCT-2 inoculant likely achieved the remediation effect in secondary salinized soil by shifting fungal community. The present findings add to the current understanding of microbial interactions in response to bacterial inoculation and can be of great significance for the application of NCT-2 inoculants in secondary salinized soil remediation. PeerJ Inc. 2021-10-12 /pmc/articles/PMC8519178/ /pubmed/34721987 http://dx.doi.org/10.7717/peerj.12309 Text en ©2021 Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Wang, Bin
Chu, Shaohua
Liu, Xiaorui
Zhang, Dan
Chai, Xiaotong
Yang, Xijia
Zhi, Yuee
Chi, Yaowei
Zhou, Pei
Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil
title Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil
title_full Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil
title_fullStr Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil
title_full_unstemmed Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil
title_short Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil
title_sort changes in soil bacterial and fungal communities in response to bacillus megaterium nct-2 inoculation in secondary salinized soil
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519178/
https://www.ncbi.nlm.nih.gov/pubmed/34721987
http://dx.doi.org/10.7717/peerj.12309
work_keys_str_mv AT wangbin changesinsoilbacterialandfungalcommunitiesinresponsetobacillusmegateriumnct2inoculationinsecondarysalinizedsoil
AT chushaohua changesinsoilbacterialandfungalcommunitiesinresponsetobacillusmegateriumnct2inoculationinsecondarysalinizedsoil
AT liuxiaorui changesinsoilbacterialandfungalcommunitiesinresponsetobacillusmegateriumnct2inoculationinsecondarysalinizedsoil
AT zhangdan changesinsoilbacterialandfungalcommunitiesinresponsetobacillusmegateriumnct2inoculationinsecondarysalinizedsoil
AT chaixiaotong changesinsoilbacterialandfungalcommunitiesinresponsetobacillusmegateriumnct2inoculationinsecondarysalinizedsoil
AT yangxijia changesinsoilbacterialandfungalcommunitiesinresponsetobacillusmegateriumnct2inoculationinsecondarysalinizedsoil
AT zhiyuee changesinsoilbacterialandfungalcommunitiesinresponsetobacillusmegateriumnct2inoculationinsecondarysalinizedsoil
AT chiyaowei changesinsoilbacterialandfungalcommunitiesinresponsetobacillusmegateriumnct2inoculationinsecondarysalinizedsoil
AT zhoupei changesinsoilbacterialandfungalcommunitiesinresponsetobacillusmegateriumnct2inoculationinsecondarysalinizedsoil