OsRELA Regulates Leaf Inclination by Repressing the Transcriptional Activity of OsLIC in Rice

Leaf angle is one of the most important agronomic traits in rice, and changes in leaf angle can alter plant architecture to affect photosynthetic efficiency and thus determine grain yield. Therefore, it is important to identify key genes controlling leaf angle and elucidate the molecular mechanisms...

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Autores principales: Zhu, Chen-li, Xing, Bao, Teng, Shou-zhen, Deng, Chen, Shen, Zhen-yong, Ai, Peng-fei, Lu, Tie-gang, Zhang, Sheng-wei, Zhang, Zhi-guo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519309/
https://www.ncbi.nlm.nih.gov/pubmed/34659326
http://dx.doi.org/10.3389/fpls.2021.760041
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author Zhu, Chen-li
Xing, Bao
Teng, Shou-zhen
Deng, Chen
Shen, Zhen-yong
Ai, Peng-fei
Lu, Tie-gang
Zhang, Sheng-wei
Zhang, Zhi-guo
author_facet Zhu, Chen-li
Xing, Bao
Teng, Shou-zhen
Deng, Chen
Shen, Zhen-yong
Ai, Peng-fei
Lu, Tie-gang
Zhang, Sheng-wei
Zhang, Zhi-guo
author_sort Zhu, Chen-li
collection PubMed
description Leaf angle is one of the most important agronomic traits in rice, and changes in leaf angle can alter plant architecture to affect photosynthetic efficiency and thus determine grain yield. Therefore, it is important to identify key genes controlling leaf angle and elucidate the molecular mechanisms to improve rice yield. We obtained a mutant rela (regulator of leaf angle) with reduced leaf angle in rice by EMS mutagenesis, and map-based cloning revealed that OsRELA encodes a protein of unknown function. Coincidentally, DENSE AND ERECT PANICLE 2 (DEP2) was reported in a previous study with the same gene locus. RNA-seq analysis revealed that OsRELA is involved in regulating the expression of ILI and Expansin family genes. Biochemical and genetic analyses revealed that OsRELA is able to interact with OsLIC, a negative regulator of BR signaling, through its conserved C-terminal domain, which is essential for OsRELA function in rice. The binding of OsRELA can activate the expression of downstream genes repressed by OsLIC, such as OsILI1, a positive regulator of leaf inclination in rice. Therefore, our results suggest that OsRELA can act as a transcriptional regulator and is involved in the regulation of leaf inclination by regulating the transcriptional activity of OsLIC.
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spelling pubmed-85193092021-10-16 OsRELA Regulates Leaf Inclination by Repressing the Transcriptional Activity of OsLIC in Rice Zhu, Chen-li Xing, Bao Teng, Shou-zhen Deng, Chen Shen, Zhen-yong Ai, Peng-fei Lu, Tie-gang Zhang, Sheng-wei Zhang, Zhi-guo Front Plant Sci Plant Science Leaf angle is one of the most important agronomic traits in rice, and changes in leaf angle can alter plant architecture to affect photosynthetic efficiency and thus determine grain yield. Therefore, it is important to identify key genes controlling leaf angle and elucidate the molecular mechanisms to improve rice yield. We obtained a mutant rela (regulator of leaf angle) with reduced leaf angle in rice by EMS mutagenesis, and map-based cloning revealed that OsRELA encodes a protein of unknown function. Coincidentally, DENSE AND ERECT PANICLE 2 (DEP2) was reported in a previous study with the same gene locus. RNA-seq analysis revealed that OsRELA is involved in regulating the expression of ILI and Expansin family genes. Biochemical and genetic analyses revealed that OsRELA is able to interact with OsLIC, a negative regulator of BR signaling, through its conserved C-terminal domain, which is essential for OsRELA function in rice. The binding of OsRELA can activate the expression of downstream genes repressed by OsLIC, such as OsILI1, a positive regulator of leaf inclination in rice. Therefore, our results suggest that OsRELA can act as a transcriptional regulator and is involved in the regulation of leaf inclination by regulating the transcriptional activity of OsLIC. Frontiers Media S.A. 2021-10-01 /pmc/articles/PMC8519309/ /pubmed/34659326 http://dx.doi.org/10.3389/fpls.2021.760041 Text en Copyright © 2021 Zhu, Xing, Teng, Deng, Shen, Ai, Lu, Zhang and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhu, Chen-li
Xing, Bao
Teng, Shou-zhen
Deng, Chen
Shen, Zhen-yong
Ai, Peng-fei
Lu, Tie-gang
Zhang, Sheng-wei
Zhang, Zhi-guo
OsRELA Regulates Leaf Inclination by Repressing the Transcriptional Activity of OsLIC in Rice
title OsRELA Regulates Leaf Inclination by Repressing the Transcriptional Activity of OsLIC in Rice
title_full OsRELA Regulates Leaf Inclination by Repressing the Transcriptional Activity of OsLIC in Rice
title_fullStr OsRELA Regulates Leaf Inclination by Repressing the Transcriptional Activity of OsLIC in Rice
title_full_unstemmed OsRELA Regulates Leaf Inclination by Repressing the Transcriptional Activity of OsLIC in Rice
title_short OsRELA Regulates Leaf Inclination by Repressing the Transcriptional Activity of OsLIC in Rice
title_sort osrela regulates leaf inclination by repressing the transcriptional activity of oslic in rice
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519309/
https://www.ncbi.nlm.nih.gov/pubmed/34659326
http://dx.doi.org/10.3389/fpls.2021.760041
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