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Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex

Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. mac...

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Autores principales: Pinotti, Heloisa, de Oliveira, Jader, Ravazi, Amanda, Madeira, Fernanda Fernandez, dos Reis, Yago Visinho, de Oliveira, Ana Beatriz Bortolozo, de Azeredo-Oliveira, Maria Tercília Vilela, da Rosa, João Aristeu, Alevi, Kaio Cesar Chaboli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519420/
https://www.ncbi.nlm.nih.gov/pubmed/34653197
http://dx.doi.org/10.1371/journal.pone.0257992
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author Pinotti, Heloisa
de Oliveira, Jader
Ravazi, Amanda
Madeira, Fernanda Fernandez
dos Reis, Yago Visinho
de Oliveira, Ana Beatriz Bortolozo
de Azeredo-Oliveira, Maria Tercília Vilela
da Rosa, João Aristeu
Alevi, Kaio Cesar Chaboli
author_facet Pinotti, Heloisa
de Oliveira, Jader
Ravazi, Amanda
Madeira, Fernanda Fernandez
dos Reis, Yago Visinho
de Oliveira, Ana Beatriz Bortolozo
de Azeredo-Oliveira, Maria Tercília Vilela
da Rosa, João Aristeu
Alevi, Kaio Cesar Chaboli
author_sort Pinotti, Heloisa
collection PubMed
description Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group.
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spelling pubmed-85194202021-10-16 Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex Pinotti, Heloisa de Oliveira, Jader Ravazi, Amanda Madeira, Fernanda Fernandez dos Reis, Yago Visinho de Oliveira, Ana Beatriz Bortolozo de Azeredo-Oliveira, Maria Tercília Vilela da Rosa, João Aristeu Alevi, Kaio Cesar Chaboli PLoS One Research Article Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group. Public Library of Science 2021-10-15 /pmc/articles/PMC8519420/ /pubmed/34653197 http://dx.doi.org/10.1371/journal.pone.0257992 Text en © 2021 Pinotti et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pinotti, Heloisa
de Oliveira, Jader
Ravazi, Amanda
Madeira, Fernanda Fernandez
dos Reis, Yago Visinho
de Oliveira, Ana Beatriz Bortolozo
de Azeredo-Oliveira, Maria Tercília Vilela
da Rosa, João Aristeu
Alevi, Kaio Cesar Chaboli
Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
title Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
title_full Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
title_fullStr Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
title_full_unstemmed Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
title_short Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
title_sort revisiting the hybridization processes in the triatoma brasiliensis complex (hemiptera, triatominae): interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519420/
https://www.ncbi.nlm.nih.gov/pubmed/34653197
http://dx.doi.org/10.1371/journal.pone.0257992
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