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The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells
The super elongation complex (SEC) has been reported to play a key role in the proliferation and differentiation of mouse embryonic stem cells. However, the expression pattern and function of the SEC in the inner ear has not been investigated. Here, we studied the inner ear expression pattern of thr...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519586/ https://www.ncbi.nlm.nih.gov/pubmed/34658793 http://dx.doi.org/10.3389/fncel.2021.735723 |
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author | Chen, Yin Qiang, Ruiying Zhang, Yuan Cao, Wei Wu, Leilei Jiang, Pei Ai, Jingru Ma, Xiangyu Dong, Ying Gao, Xia Li, He Lu, Ling Zhang, Shasha Chai, Renjie |
author_facet | Chen, Yin Qiang, Ruiying Zhang, Yuan Cao, Wei Wu, Leilei Jiang, Pei Ai, Jingru Ma, Xiangyu Dong, Ying Gao, Xia Li, He Lu, Ling Zhang, Shasha Chai, Renjie |
author_sort | Chen, Yin |
collection | PubMed |
description | The super elongation complex (SEC) has been reported to play a key role in the proliferation and differentiation of mouse embryonic stem cells. However, the expression pattern and function of the SEC in the inner ear has not been investigated. Here, we studied the inner ear expression pattern of three key SEC components, AFF1, AFF4, and ELL3, and found that these three proteins are all expressed in both cochlear hair cells (HCs)and supporting cells (SCs). We also cultured Lgr5+ inner ear progenitors in vitro for sphere-forming assays and differentiation assays in the presence of the SEC inhibitor flavopiridol. We found that flavopiridol treatment decreased the proliferation ability of Lgr5+ progenitors, while the differentiation ability of Lgr5+ progenitors was not affected. Our results suggest that the SEC might play important roles in regulating inner ear progenitors and thus regulating HC regeneration. Therefore, it will be very meaningful to further investigate the detailed roles of the SEC signaling pathway in the inner ear in vivo in order to develop effective treatments for sensorineural hearing loss. |
format | Online Article Text |
id | pubmed-8519586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85195862021-10-16 The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells Chen, Yin Qiang, Ruiying Zhang, Yuan Cao, Wei Wu, Leilei Jiang, Pei Ai, Jingru Ma, Xiangyu Dong, Ying Gao, Xia Li, He Lu, Ling Zhang, Shasha Chai, Renjie Front Cell Neurosci Cellular Neuroscience The super elongation complex (SEC) has been reported to play a key role in the proliferation and differentiation of mouse embryonic stem cells. However, the expression pattern and function of the SEC in the inner ear has not been investigated. Here, we studied the inner ear expression pattern of three key SEC components, AFF1, AFF4, and ELL3, and found that these three proteins are all expressed in both cochlear hair cells (HCs)and supporting cells (SCs). We also cultured Lgr5+ inner ear progenitors in vitro for sphere-forming assays and differentiation assays in the presence of the SEC inhibitor flavopiridol. We found that flavopiridol treatment decreased the proliferation ability of Lgr5+ progenitors, while the differentiation ability of Lgr5+ progenitors was not affected. Our results suggest that the SEC might play important roles in regulating inner ear progenitors and thus regulating HC regeneration. Therefore, it will be very meaningful to further investigate the detailed roles of the SEC signaling pathway in the inner ear in vivo in order to develop effective treatments for sensorineural hearing loss. Frontiers Media S.A. 2021-10-01 /pmc/articles/PMC8519586/ /pubmed/34658793 http://dx.doi.org/10.3389/fncel.2021.735723 Text en Copyright © 2021 Chen, Qiang, Zhang, Cao, Wu, Jiang, Ai, Ma, Dong, Gao, Li, Lu, Zhang and Chai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular Neuroscience Chen, Yin Qiang, Ruiying Zhang, Yuan Cao, Wei Wu, Leilei Jiang, Pei Ai, Jingru Ma, Xiangyu Dong, Ying Gao, Xia Li, He Lu, Ling Zhang, Shasha Chai, Renjie The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells |
title | The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells |
title_full | The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells |
title_fullStr | The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells |
title_full_unstemmed | The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells |
title_short | The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells |
title_sort | expression and roles of the super elongation complex in mouse cochlear lgr5+ progenitor cells |
topic | Cellular Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519586/ https://www.ncbi.nlm.nih.gov/pubmed/34658793 http://dx.doi.org/10.3389/fncel.2021.735723 |
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