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Increased circulating platelet and endothelial-derived microparticles in patients with cardiac syndrome X

BACKGROUND: Cardiac syndrome X (CSX) has been associated with endothelial dysfunction and inflammation. We conducted a case-control study to evaluate the association between plateletý and endothelial-derived microparticles (PMPs and EMPs), as specific quantitative plasma markers of endothelial dysfu...

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Autores principales: Ghaffari, Fereshteh, Rasmi, Yousef, Seyed Mohammadzad, Mir Hossein, Seyedi, Shahram, Shirpoor, Alireza, Roshani-Asl, Elmira, Saboory, Ehsan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Isfahan Cardiovascular Research Center, Isfahan University of Medical Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519618/
https://www.ncbi.nlm.nih.gov/pubmed/34703482
http://dx.doi.org/10.22122/arya.v17i0.2094
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author Ghaffari, Fereshteh
Rasmi, Yousef
Seyed Mohammadzad, Mir Hossein
Seyedi, Shahram
Shirpoor, Alireza
Roshani-Asl, Elmira
Saboory, Ehsan
author_facet Ghaffari, Fereshteh
Rasmi, Yousef
Seyed Mohammadzad, Mir Hossein
Seyedi, Shahram
Shirpoor, Alireza
Roshani-Asl, Elmira
Saboory, Ehsan
author_sort Ghaffari, Fereshteh
collection PubMed
description BACKGROUND: Cardiac syndrome X (CSX) has been associated with endothelial dysfunction and inflammation. We conducted a case-control study to evaluate the association between plateletý and endothelial-derived microparticles (PMPs and EMPs), as specific quantitative plasma markers of endothelial dysfunction, and the presence of CSX. METHODS: The present study was conducted on 40 CSX patients and 19 healthy individuals. C-reactive protein (CRP), and hematological and biochemical parameters were evaluated. The MP concentration in platelet-poor plasma (PPP) was quantitatively determined through flow cytometry using specific anti-human CD31, CD41a, CD62E, and CD144 antibodies. RESULTS: The mean platelet volume (MPV) and positive CRP rate (≥ 3.8 mg/l) were higher in patients compared to controls (P = 0.020 and P = 0.010, respectively). The CD62E+, CD144+, and CD31+41− EMPs, as well as CD41+ and CD31+CD41+ PMPs showed significant increase in CSX patients compared to controls (P < 0.050). There were direct correlations between the mean percentage of detected EMPs and PMPs as well as between their expression intensity; however, a reverse correlation was seen between the percentage of MPs and CD144 and CD41. Moreover, the MP level was reversely associated with prothrombin time (PT) and partial thromboplastin time (PTT) values. Only CD31+CD41+ PMP was correlated with CRP. CONCLUSION: It seems that EMPs and PMPs increase in CSX, which may contribute to various processes involved in the development of this syndrome.
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spelling pubmed-85196182021-10-25 Increased circulating platelet and endothelial-derived microparticles in patients with cardiac syndrome X Ghaffari, Fereshteh Rasmi, Yousef Seyed Mohammadzad, Mir Hossein Seyedi, Shahram Shirpoor, Alireza Roshani-Asl, Elmira Saboory, Ehsan ARYA Atheroscler Original Article BACKGROUND: Cardiac syndrome X (CSX) has been associated with endothelial dysfunction and inflammation. We conducted a case-control study to evaluate the association between plateletý and endothelial-derived microparticles (PMPs and EMPs), as specific quantitative plasma markers of endothelial dysfunction, and the presence of CSX. METHODS: The present study was conducted on 40 CSX patients and 19 healthy individuals. C-reactive protein (CRP), and hematological and biochemical parameters were evaluated. The MP concentration in platelet-poor plasma (PPP) was quantitatively determined through flow cytometry using specific anti-human CD31, CD41a, CD62E, and CD144 antibodies. RESULTS: The mean platelet volume (MPV) and positive CRP rate (≥ 3.8 mg/l) were higher in patients compared to controls (P = 0.020 and P = 0.010, respectively). The CD62E+, CD144+, and CD31+41− EMPs, as well as CD41+ and CD31+CD41+ PMPs showed significant increase in CSX patients compared to controls (P < 0.050). There were direct correlations between the mean percentage of detected EMPs and PMPs as well as between their expression intensity; however, a reverse correlation was seen between the percentage of MPs and CD144 and CD41. Moreover, the MP level was reversely associated with prothrombin time (PT) and partial thromboplastin time (PTT) values. Only CD31+CD41+ PMP was correlated with CRP. CONCLUSION: It seems that EMPs and PMPs increase in CSX, which may contribute to various processes involved in the development of this syndrome. Isfahan Cardiovascular Research Center, Isfahan University of Medical Sciences 2021-01 /pmc/articles/PMC8519618/ /pubmed/34703482 http://dx.doi.org/10.22122/arya.v17i0.2094 Text en © 2021 Isfahan Cardiovascular Research Center & Isfahan University of Medical Sciences https://creativecommons.org/licenses/by-nc/3.0/This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Ghaffari, Fereshteh
Rasmi, Yousef
Seyed Mohammadzad, Mir Hossein
Seyedi, Shahram
Shirpoor, Alireza
Roshani-Asl, Elmira
Saboory, Ehsan
Increased circulating platelet and endothelial-derived microparticles in patients with cardiac syndrome X
title Increased circulating platelet and endothelial-derived microparticles in patients with cardiac syndrome X
title_full Increased circulating platelet and endothelial-derived microparticles in patients with cardiac syndrome X
title_fullStr Increased circulating platelet and endothelial-derived microparticles in patients with cardiac syndrome X
title_full_unstemmed Increased circulating platelet and endothelial-derived microparticles in patients with cardiac syndrome X
title_short Increased circulating platelet and endothelial-derived microparticles in patients with cardiac syndrome X
title_sort increased circulating platelet and endothelial-derived microparticles in patients with cardiac syndrome x
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519618/
https://www.ncbi.nlm.nih.gov/pubmed/34703482
http://dx.doi.org/10.22122/arya.v17i0.2094
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