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A needle tip CCEA microfluidic device based on enhanced Dean flow for cell washing
Particle/cell washing is an essential technique in biological and clinical manipulations. Herein, we propose a novel circular contraction–expansion array (CCEA) microdevice. It can be directly connected to a needle tip without connection tubes. Its small size and centrosymmetric structure are benefi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519928/ https://www.ncbi.nlm.nih.gov/pubmed/34721889 http://dx.doi.org/10.1038/s41378-021-00311-9 |
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author | Shi, Xin Tan, Wei Lu, Yuwen Cao, Wenfeng Zhu, Guorui |
author_facet | Shi, Xin Tan, Wei Lu, Yuwen Cao, Wenfeng Zhu, Guorui |
author_sort | Shi, Xin |
collection | PubMed |
description | Particle/cell washing is an essential technique in biological and clinical manipulations. Herein, we propose a novel circular contraction–expansion array (CCEA) microdevice. It can be directly connected to a needle tip without connection tubes. Its small size and centrosymmetric structure are beneficial to low sample consumption, high connection stability, and a wide application range. Computational fluid dynamics (CFD) simulation results show that the CCEA structure can produce a stronger Dean flow and lead to faster particle/cell focusing than the circle structure and CEA structure with the same length. Experimentally, an optimal flow rate ratio of 1:3 and an optimal total flow rate of 120 μL/min were found to ensure a stable fluid distribution. Under these conditions, rapid focusing of 10–20 μm particles with high efficiencies was achieved. Compared with a normal CEA device using tubes, the particle loss rate could be reduced from 64 to 7% when washing 500 μL of a rare sample. Cell suspensions with concentrations from 3 × 10(5)/mL to 1 × 10(3)/mL were tested. The high cell collection efficiency (>85% for three cell lines) and stable waste removal efficiency (>80%) reflected the universality of the CCEA microfluidic device. After the washing, the cell activities of H1299 cells and MCF-7 cells were calculated to be 93.8 and 97.5%, respectively. This needle-tip CCEA microfluidic device showed potential in basic medical research and clinical diagnosis. |
format | Online Article Text |
id | pubmed-8519928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85199282021-10-29 A needle tip CCEA microfluidic device based on enhanced Dean flow for cell washing Shi, Xin Tan, Wei Lu, Yuwen Cao, Wenfeng Zhu, Guorui Microsyst Nanoeng Article Particle/cell washing is an essential technique in biological and clinical manipulations. Herein, we propose a novel circular contraction–expansion array (CCEA) microdevice. It can be directly connected to a needle tip without connection tubes. Its small size and centrosymmetric structure are beneficial to low sample consumption, high connection stability, and a wide application range. Computational fluid dynamics (CFD) simulation results show that the CCEA structure can produce a stronger Dean flow and lead to faster particle/cell focusing than the circle structure and CEA structure with the same length. Experimentally, an optimal flow rate ratio of 1:3 and an optimal total flow rate of 120 μL/min were found to ensure a stable fluid distribution. Under these conditions, rapid focusing of 10–20 μm particles with high efficiencies was achieved. Compared with a normal CEA device using tubes, the particle loss rate could be reduced from 64 to 7% when washing 500 μL of a rare sample. Cell suspensions with concentrations from 3 × 10(5)/mL to 1 × 10(3)/mL were tested. The high cell collection efficiency (>85% for three cell lines) and stable waste removal efficiency (>80%) reflected the universality of the CCEA microfluidic device. After the washing, the cell activities of H1299 cells and MCF-7 cells were calculated to be 93.8 and 97.5%, respectively. This needle-tip CCEA microfluidic device showed potential in basic medical research and clinical diagnosis. Nature Publishing Group UK 2021-10-15 /pmc/articles/PMC8519928/ /pubmed/34721889 http://dx.doi.org/10.1038/s41378-021-00311-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Shi, Xin Tan, Wei Lu, Yuwen Cao, Wenfeng Zhu, Guorui A needle tip CCEA microfluidic device based on enhanced Dean flow for cell washing |
title | A needle tip CCEA microfluidic device based on enhanced Dean flow for cell washing |
title_full | A needle tip CCEA microfluidic device based on enhanced Dean flow for cell washing |
title_fullStr | A needle tip CCEA microfluidic device based on enhanced Dean flow for cell washing |
title_full_unstemmed | A needle tip CCEA microfluidic device based on enhanced Dean flow for cell washing |
title_short | A needle tip CCEA microfluidic device based on enhanced Dean flow for cell washing |
title_sort | needle tip ccea microfluidic device based on enhanced dean flow for cell washing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519928/ https://www.ncbi.nlm.nih.gov/pubmed/34721889 http://dx.doi.org/10.1038/s41378-021-00311-9 |
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