Cargando…

Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment

Lymphangiogenesis is a stage of new lymphatic vessel formation in development and pathology, such as inflammation and tumor metastasis. Physiologically relevant models of lymphatic vessels have been in demand because studies on lymphatic vessels are required for understanding the mechanism of tumor...

Descripción completa

Detalles Bibliográficos
Autores principales: Cho, Youngkyu, Na, Kyuhwan, Jun, Yesl, Won, Jihee, Yang, Ji Hun, Chung, Seok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8520924/
https://www.ncbi.nlm.nih.gov/pubmed/34671596
http://dx.doi.org/10.3389/fbioe.2021.697657
_version_ 1784584785816977408
author Cho, Youngkyu
Na, Kyuhwan
Jun, Yesl
Won, Jihee
Yang, Ji Hun
Chung, Seok
author_facet Cho, Youngkyu
Na, Kyuhwan
Jun, Yesl
Won, Jihee
Yang, Ji Hun
Chung, Seok
author_sort Cho, Youngkyu
collection PubMed
description Lymphangiogenesis is a stage of new lymphatic vessel formation in development and pathology, such as inflammation and tumor metastasis. Physiologically relevant models of lymphatic vessels have been in demand because studies on lymphatic vessels are required for understanding the mechanism of tumor metastasis. In this study, a new three-dimensional lymphangiogenesis model in a tumor microenvironment is proposed, using a newly designed macrofluidic platform. It is verified that controllable biochemical and biomechanical cues, which contribute to lymphangiogenesis, can be applied in this platform. In particular, this model demonstrates that a reconstituted lymphatic vessel has an in vivo–like lymphatic vessel in both physical and biochemical aspects. Since biomechanical stress with a biochemical factor influences robust directional lymphatic sprouting, whether our model closely approximates in vivo, the initial lymphatics in terms of the morphological and genetic signatures is investigated. Furthermore, attempting an incorporation with a tumor spheroid, this study successfully develops a complex tumor microenvironment model for use in lymphangiogenesis and reveals the microenvironment factors that contribute to tumor metastasis. As a first attempt at a coculture model, this reconstituted model is a novel system with a fully three-dimensional structure and can be a powerful tool for pathological drug screening or disease model.
format Online
Article
Text
id pubmed-8520924
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85209242021-10-19 Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment Cho, Youngkyu Na, Kyuhwan Jun, Yesl Won, Jihee Yang, Ji Hun Chung, Seok Front Bioeng Biotechnol Bioengineering and Biotechnology Lymphangiogenesis is a stage of new lymphatic vessel formation in development and pathology, such as inflammation and tumor metastasis. Physiologically relevant models of lymphatic vessels have been in demand because studies on lymphatic vessels are required for understanding the mechanism of tumor metastasis. In this study, a new three-dimensional lymphangiogenesis model in a tumor microenvironment is proposed, using a newly designed macrofluidic platform. It is verified that controllable biochemical and biomechanical cues, which contribute to lymphangiogenesis, can be applied in this platform. In particular, this model demonstrates that a reconstituted lymphatic vessel has an in vivo–like lymphatic vessel in both physical and biochemical aspects. Since biomechanical stress with a biochemical factor influences robust directional lymphatic sprouting, whether our model closely approximates in vivo, the initial lymphatics in terms of the morphological and genetic signatures is investigated. Furthermore, attempting an incorporation with a tumor spheroid, this study successfully develops a complex tumor microenvironment model for use in lymphangiogenesis and reveals the microenvironment factors that contribute to tumor metastasis. As a first attempt at a coculture model, this reconstituted model is a novel system with a fully three-dimensional structure and can be a powerful tool for pathological drug screening or disease model. Frontiers Media S.A. 2021-10-04 /pmc/articles/PMC8520924/ /pubmed/34671596 http://dx.doi.org/10.3389/fbioe.2021.697657 Text en Copyright © 2021 Cho, Na, Jun, Won, Yang and Chung. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Cho, Youngkyu
Na, Kyuhwan
Jun, Yesl
Won, Jihee
Yang, Ji Hun
Chung, Seok
Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment
title Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment
title_full Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment
title_fullStr Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment
title_full_unstemmed Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment
title_short Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment
title_sort three-dimensional in vitro lymphangiogenesis model in tumor microenvironment
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8520924/
https://www.ncbi.nlm.nih.gov/pubmed/34671596
http://dx.doi.org/10.3389/fbioe.2021.697657
work_keys_str_mv AT choyoungkyu threedimensionalinvitrolymphangiogenesismodelintumormicroenvironment
AT nakyuhwan threedimensionalinvitrolymphangiogenesismodelintumormicroenvironment
AT junyesl threedimensionalinvitrolymphangiogenesismodelintumormicroenvironment
AT wonjihee threedimensionalinvitrolymphangiogenesismodelintumormicroenvironment
AT yangjihun threedimensionalinvitrolymphangiogenesismodelintumormicroenvironment
AT chungseok threedimensionalinvitrolymphangiogenesismodelintumormicroenvironment