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Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment
Lymphangiogenesis is a stage of new lymphatic vessel formation in development and pathology, such as inflammation and tumor metastasis. Physiologically relevant models of lymphatic vessels have been in demand because studies on lymphatic vessels are required for understanding the mechanism of tumor...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8520924/ https://www.ncbi.nlm.nih.gov/pubmed/34671596 http://dx.doi.org/10.3389/fbioe.2021.697657 |
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author | Cho, Youngkyu Na, Kyuhwan Jun, Yesl Won, Jihee Yang, Ji Hun Chung, Seok |
author_facet | Cho, Youngkyu Na, Kyuhwan Jun, Yesl Won, Jihee Yang, Ji Hun Chung, Seok |
author_sort | Cho, Youngkyu |
collection | PubMed |
description | Lymphangiogenesis is a stage of new lymphatic vessel formation in development and pathology, such as inflammation and tumor metastasis. Physiologically relevant models of lymphatic vessels have been in demand because studies on lymphatic vessels are required for understanding the mechanism of tumor metastasis. In this study, a new three-dimensional lymphangiogenesis model in a tumor microenvironment is proposed, using a newly designed macrofluidic platform. It is verified that controllable biochemical and biomechanical cues, which contribute to lymphangiogenesis, can be applied in this platform. In particular, this model demonstrates that a reconstituted lymphatic vessel has an in vivo–like lymphatic vessel in both physical and biochemical aspects. Since biomechanical stress with a biochemical factor influences robust directional lymphatic sprouting, whether our model closely approximates in vivo, the initial lymphatics in terms of the morphological and genetic signatures is investigated. Furthermore, attempting an incorporation with a tumor spheroid, this study successfully develops a complex tumor microenvironment model for use in lymphangiogenesis and reveals the microenvironment factors that contribute to tumor metastasis. As a first attempt at a coculture model, this reconstituted model is a novel system with a fully three-dimensional structure and can be a powerful tool for pathological drug screening or disease model. |
format | Online Article Text |
id | pubmed-8520924 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85209242021-10-19 Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment Cho, Youngkyu Na, Kyuhwan Jun, Yesl Won, Jihee Yang, Ji Hun Chung, Seok Front Bioeng Biotechnol Bioengineering and Biotechnology Lymphangiogenesis is a stage of new lymphatic vessel formation in development and pathology, such as inflammation and tumor metastasis. Physiologically relevant models of lymphatic vessels have been in demand because studies on lymphatic vessels are required for understanding the mechanism of tumor metastasis. In this study, a new three-dimensional lymphangiogenesis model in a tumor microenvironment is proposed, using a newly designed macrofluidic platform. It is verified that controllable biochemical and biomechanical cues, which contribute to lymphangiogenesis, can be applied in this platform. In particular, this model demonstrates that a reconstituted lymphatic vessel has an in vivo–like lymphatic vessel in both physical and biochemical aspects. Since biomechanical stress with a biochemical factor influences robust directional lymphatic sprouting, whether our model closely approximates in vivo, the initial lymphatics in terms of the morphological and genetic signatures is investigated. Furthermore, attempting an incorporation with a tumor spheroid, this study successfully develops a complex tumor microenvironment model for use in lymphangiogenesis and reveals the microenvironment factors that contribute to tumor metastasis. As a first attempt at a coculture model, this reconstituted model is a novel system with a fully three-dimensional structure and can be a powerful tool for pathological drug screening or disease model. Frontiers Media S.A. 2021-10-04 /pmc/articles/PMC8520924/ /pubmed/34671596 http://dx.doi.org/10.3389/fbioe.2021.697657 Text en Copyright © 2021 Cho, Na, Jun, Won, Yang and Chung. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Cho, Youngkyu Na, Kyuhwan Jun, Yesl Won, Jihee Yang, Ji Hun Chung, Seok Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment |
title | Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment |
title_full | Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment |
title_fullStr | Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment |
title_full_unstemmed | Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment |
title_short | Three-Dimensional In Vitro Lymphangiogenesis Model in Tumor Microenvironment |
title_sort | three-dimensional in vitro lymphangiogenesis model in tumor microenvironment |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8520924/ https://www.ncbi.nlm.nih.gov/pubmed/34671596 http://dx.doi.org/10.3389/fbioe.2021.697657 |
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