Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics

The MGISEQ-2000 sequencer is widely used in various omics studies, but the performance of this platform for paleogenomics has not been evaluated. We here compare the performance of MGISEQ-2000 with the Illumina X-Ten on ancient human DNA using four samples from 1750BCE to 60CE. We found there were o...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Kongyang, Du, Panxin, Xiong, Jianxue, Ren, Xiaoying, Sun, Chang, Tao, Yichen, Ding, Yi, Xu, Yiran, Meng, Hailiang, Wang, Chuan-Chao, Wen, Shao-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521044/
https://www.ncbi.nlm.nih.gov/pubmed/34671385
http://dx.doi.org/10.3389/fgene.2021.745508
_version_ 1784584815360606208
author Zhu, Kongyang
Du, Panxin
Xiong, Jianxue
Ren, Xiaoying
Sun, Chang
Tao, Yichen
Ding, Yi
Xu, Yiran
Meng, Hailiang
Wang, Chuan-Chao
Wen, Shao-Qing
author_facet Zhu, Kongyang
Du, Panxin
Xiong, Jianxue
Ren, Xiaoying
Sun, Chang
Tao, Yichen
Ding, Yi
Xu, Yiran
Meng, Hailiang
Wang, Chuan-Chao
Wen, Shao-Qing
author_sort Zhu, Kongyang
collection PubMed
description The MGISEQ-2000 sequencer is widely used in various omics studies, but the performance of this platform for paleogenomics has not been evaluated. We here compare the performance of MGISEQ-2000 with the Illumina X-Ten on ancient human DNA using four samples from 1750BCE to 60CE. We found there were only slight differences between the two platforms in most parameters (duplication rate, sequencing bias, θ, δS, and λ). MGISEQ-2000 performed well on endogenous rate and library complexity although X-Ten had a higher average base quality and lower error rate. Our results suggest that MGISEQ-2000 and X-Ten have comparable performance, and MGISEQ-2000 can be an alternative platform for paleogenomics sequencing.
format Online
Article
Text
id pubmed-8521044
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85210442021-10-19 Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics Zhu, Kongyang Du, Panxin Xiong, Jianxue Ren, Xiaoying Sun, Chang Tao, Yichen Ding, Yi Xu, Yiran Meng, Hailiang Wang, Chuan-Chao Wen, Shao-Qing Front Genet Genetics The MGISEQ-2000 sequencer is widely used in various omics studies, but the performance of this platform for paleogenomics has not been evaluated. We here compare the performance of MGISEQ-2000 with the Illumina X-Ten on ancient human DNA using four samples from 1750BCE to 60CE. We found there were only slight differences between the two platforms in most parameters (duplication rate, sequencing bias, θ, δS, and λ). MGISEQ-2000 performed well on endogenous rate and library complexity although X-Ten had a higher average base quality and lower error rate. Our results suggest that MGISEQ-2000 and X-Ten have comparable performance, and MGISEQ-2000 can be an alternative platform for paleogenomics sequencing. Frontiers Media S.A. 2021-10-04 /pmc/articles/PMC8521044/ /pubmed/34671385 http://dx.doi.org/10.3389/fgene.2021.745508 Text en Copyright © 2021 Zhu, Du, Xiong, Ren, Sun, Tao, Ding, Xu, Meng, Wang and Wen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhu, Kongyang
Du, Panxin
Xiong, Jianxue
Ren, Xiaoying
Sun, Chang
Tao, Yichen
Ding, Yi
Xu, Yiran
Meng, Hailiang
Wang, Chuan-Chao
Wen, Shao-Qing
Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics
title Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics
title_full Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics
title_fullStr Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics
title_full_unstemmed Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics
title_short Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics
title_sort comparative performance of the mgiseq-2000 and illumina x-ten sequencing platforms for paleogenomics
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521044/
https://www.ncbi.nlm.nih.gov/pubmed/34671385
http://dx.doi.org/10.3389/fgene.2021.745508
work_keys_str_mv AT zhukongyang comparativeperformanceofthemgiseq2000andilluminaxtensequencingplatformsforpaleogenomics
AT dupanxin comparativeperformanceofthemgiseq2000andilluminaxtensequencingplatformsforpaleogenomics
AT xiongjianxue comparativeperformanceofthemgiseq2000andilluminaxtensequencingplatformsforpaleogenomics
AT renxiaoying comparativeperformanceofthemgiseq2000andilluminaxtensequencingplatformsforpaleogenomics
AT sunchang comparativeperformanceofthemgiseq2000andilluminaxtensequencingplatformsforpaleogenomics
AT taoyichen comparativeperformanceofthemgiseq2000andilluminaxtensequencingplatformsforpaleogenomics
AT dingyi comparativeperformanceofthemgiseq2000andilluminaxtensequencingplatformsforpaleogenomics
AT xuyiran comparativeperformanceofthemgiseq2000andilluminaxtensequencingplatformsforpaleogenomics
AT menghailiang comparativeperformanceofthemgiseq2000andilluminaxtensequencingplatformsforpaleogenomics
AT wangchuanchao comparativeperformanceofthemgiseq2000andilluminaxtensequencingplatformsforpaleogenomics
AT wenshaoqing comparativeperformanceofthemgiseq2000andilluminaxtensequencingplatformsforpaleogenomics

Ejemplares similares