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Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies)
We describe a general method that allows structure determination of small proteins by single-particle cryo-electron microscopy (cryo-EM). The method is based on the availability of a target-binding nanobody, which is then rigidly attached to two scaffolds: 1) a Fab fragment of an antibody directed a...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
National Academy of Sciences
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521671/ https://www.ncbi.nlm.nih.gov/pubmed/34620716 http://dx.doi.org/10.1073/pnas.2115001118 |