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Imaging and Localization of Single Emitters near Plasmonic Particles of Different Size, Shape, and Material

[Image: see text] Colloidal plasmonic materials are increasingly used in biosensing and catalysis, which has sparked the use of super-resolution localization microscopy to visualize processes at the interface of the particles. We quantify the effect of particle–emitter coupling on super-resolution l...

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Detalles Bibliográficos
Autores principales: Bloksma, Frank, Zijlstra, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521989/
https://www.ncbi.nlm.nih.gov/pubmed/34676018
http://dx.doi.org/10.1021/acs.jpcc.1c06665
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author Bloksma, Frank
Zijlstra, Peter
author_facet Bloksma, Frank
Zijlstra, Peter
author_sort Bloksma, Frank
collection PubMed
description [Image: see text] Colloidal plasmonic materials are increasingly used in biosensing and catalysis, which has sparked the use of super-resolution localization microscopy to visualize processes at the interface of the particles. We quantify the effect of particle–emitter coupling on super-resolution localization accuracy by simulating the point spread function (PSF) of single emitters near a plasmonic nanoparticle. Using a computationally inexpensive boundary element method, we investigate a broad range of conditions allowing us to compare the simulated localization accuracy to reported experimental results. We identify regimes where the PSF is not Gaussian anymore, resulting in large mislocalizations due to the appearance of multilobed PSFs. Such exotic PSFs occur when near-field excitation of quadrupole plasmons is efficient but unexpectedly also occur for large particle–emitter spacing where the coherent emission from the particle and emitter results in anisotropic emission patterns. We provide guidelines to enable faithful localization microscopy near colloidal plasmonic materials, which indicate that simply decreasing the coupling between particle and molecule is not sufficient for faithful super-resolution imaging.
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spelling pubmed-85219892021-10-19 Imaging and Localization of Single Emitters near Plasmonic Particles of Different Size, Shape, and Material Bloksma, Frank Zijlstra, Peter J Phys Chem C Nanomater Interfaces [Image: see text] Colloidal plasmonic materials are increasingly used in biosensing and catalysis, which has sparked the use of super-resolution localization microscopy to visualize processes at the interface of the particles. We quantify the effect of particle–emitter coupling on super-resolution localization accuracy by simulating the point spread function (PSF) of single emitters near a plasmonic nanoparticle. Using a computationally inexpensive boundary element method, we investigate a broad range of conditions allowing us to compare the simulated localization accuracy to reported experimental results. We identify regimes where the PSF is not Gaussian anymore, resulting in large mislocalizations due to the appearance of multilobed PSFs. Such exotic PSFs occur when near-field excitation of quadrupole plasmons is efficient but unexpectedly also occur for large particle–emitter spacing where the coherent emission from the particle and emitter results in anisotropic emission patterns. We provide guidelines to enable faithful localization microscopy near colloidal plasmonic materials, which indicate that simply decreasing the coupling between particle and molecule is not sufficient for faithful super-resolution imaging. American Chemical Society 2021-10-05 2021-10-14 /pmc/articles/PMC8521989/ /pubmed/34676018 http://dx.doi.org/10.1021/acs.jpcc.1c06665 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Bloksma, Frank
Zijlstra, Peter
Imaging and Localization of Single Emitters near Plasmonic Particles of Different Size, Shape, and Material
title Imaging and Localization of Single Emitters near Plasmonic Particles of Different Size, Shape, and Material
title_full Imaging and Localization of Single Emitters near Plasmonic Particles of Different Size, Shape, and Material
title_fullStr Imaging and Localization of Single Emitters near Plasmonic Particles of Different Size, Shape, and Material
title_full_unstemmed Imaging and Localization of Single Emitters near Plasmonic Particles of Different Size, Shape, and Material
title_short Imaging and Localization of Single Emitters near Plasmonic Particles of Different Size, Shape, and Material
title_sort imaging and localization of single emitters near plasmonic particles of different size, shape, and material
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521989/
https://www.ncbi.nlm.nih.gov/pubmed/34676018
http://dx.doi.org/10.1021/acs.jpcc.1c06665
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