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Unique Genomic Alterations of Cerebrospinal Fluid Cell-Free DNA Are Critical for Targeted Therapy of Non-Small Cell Lung Cancer With Leptomeningeal Metastasis

We reported unique molecular features of cerebrospinal fluid (CSF) of nonsmall cell lung cancer (NSCLC) patients with leptomeningeal metastasis (LM), suggesting establishing CSF as a better liquid biopsy in clinical practices. We performed next-generation panel sequencing of primary tumor tissue, pl...

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Autores principales: Wang, Yongsheng, Jiang, Feng, Xia, Ruixue, Li, Ming, Yao, Chengyun, Li, Yan, Li, Hui, Zhao, Qi, Shi, Mingke, Yu, Yanzhe, Shao, Yang W., Zhou, Guoren, Xia, Hongping, Miao, Liyun, Cai, Hourong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522975/
https://www.ncbi.nlm.nih.gov/pubmed/34671549
http://dx.doi.org/10.3389/fonc.2021.701171
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author Wang, Yongsheng
Jiang, Feng
Xia, Ruixue
Li, Ming
Yao, Chengyun
Li, Yan
Li, Hui
Zhao, Qi
Shi, Mingke
Yu, Yanzhe
Shao, Yang W.
Zhou, Guoren
Xia, Hongping
Miao, Liyun
Cai, Hourong
author_facet Wang, Yongsheng
Jiang, Feng
Xia, Ruixue
Li, Ming
Yao, Chengyun
Li, Yan
Li, Hui
Zhao, Qi
Shi, Mingke
Yu, Yanzhe
Shao, Yang W.
Zhou, Guoren
Xia, Hongping
Miao, Liyun
Cai, Hourong
author_sort Wang, Yongsheng
collection PubMed
description We reported unique molecular features of cerebrospinal fluid (CSF) of nonsmall cell lung cancer (NSCLC) patients with leptomeningeal metastasis (LM), suggesting establishing CSF as a better liquid biopsy in clinical practices. We performed next-generation panel sequencing of primary tumor tissue, plasma, and CSF from 131 NSCLC patients with LM and observed high somatic copy number variations (CNV) in CSF of NSCLC patients with LM. The status of EGFR-activating mutations was highly concordant between CSF, plasma, and primary tumors. ALK translocation was detected in 8.3% of tumor tissues but only 2.4% in CSF and 2.7% in plasma. Others such as ROS1 rearrangement, RET fusion, HER2 mutation, NTRK1 fusion, and BRAF V600E mutation were detected in 7.9% of CSF and 11.1% of tumor tissues but only 4% in plasma. Our study has shed light on the unique genomic variations of CSF and demonstrated that CSF might represent better liquid biopsy for NSCLC patients with LM.
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spelling pubmed-85229752021-10-19 Unique Genomic Alterations of Cerebrospinal Fluid Cell-Free DNA Are Critical for Targeted Therapy of Non-Small Cell Lung Cancer With Leptomeningeal Metastasis Wang, Yongsheng Jiang, Feng Xia, Ruixue Li, Ming Yao, Chengyun Li, Yan Li, Hui Zhao, Qi Shi, Mingke Yu, Yanzhe Shao, Yang W. Zhou, Guoren Xia, Hongping Miao, Liyun Cai, Hourong Front Oncol Oncology We reported unique molecular features of cerebrospinal fluid (CSF) of nonsmall cell lung cancer (NSCLC) patients with leptomeningeal metastasis (LM), suggesting establishing CSF as a better liquid biopsy in clinical practices. We performed next-generation panel sequencing of primary tumor tissue, plasma, and CSF from 131 NSCLC patients with LM and observed high somatic copy number variations (CNV) in CSF of NSCLC patients with LM. The status of EGFR-activating mutations was highly concordant between CSF, plasma, and primary tumors. ALK translocation was detected in 8.3% of tumor tissues but only 2.4% in CSF and 2.7% in plasma. Others such as ROS1 rearrangement, RET fusion, HER2 mutation, NTRK1 fusion, and BRAF V600E mutation were detected in 7.9% of CSF and 11.1% of tumor tissues but only 4% in plasma. Our study has shed light on the unique genomic variations of CSF and demonstrated that CSF might represent better liquid biopsy for NSCLC patients with LM. Frontiers Media S.A. 2021-10-04 /pmc/articles/PMC8522975/ /pubmed/34671549 http://dx.doi.org/10.3389/fonc.2021.701171 Text en Copyright © 2021 Wang, Jiang, Xia, Li, Yao, Li, Li, Zhao, Shi, Yu, Shao, Zhou, Xia, Miao and Cai https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Wang, Yongsheng
Jiang, Feng
Xia, Ruixue
Li, Ming
Yao, Chengyun
Li, Yan
Li, Hui
Zhao, Qi
Shi, Mingke
Yu, Yanzhe
Shao, Yang W.
Zhou, Guoren
Xia, Hongping
Miao, Liyun
Cai, Hourong
Unique Genomic Alterations of Cerebrospinal Fluid Cell-Free DNA Are Critical for Targeted Therapy of Non-Small Cell Lung Cancer With Leptomeningeal Metastasis
title Unique Genomic Alterations of Cerebrospinal Fluid Cell-Free DNA Are Critical for Targeted Therapy of Non-Small Cell Lung Cancer With Leptomeningeal Metastasis
title_full Unique Genomic Alterations of Cerebrospinal Fluid Cell-Free DNA Are Critical for Targeted Therapy of Non-Small Cell Lung Cancer With Leptomeningeal Metastasis
title_fullStr Unique Genomic Alterations of Cerebrospinal Fluid Cell-Free DNA Are Critical for Targeted Therapy of Non-Small Cell Lung Cancer With Leptomeningeal Metastasis
title_full_unstemmed Unique Genomic Alterations of Cerebrospinal Fluid Cell-Free DNA Are Critical for Targeted Therapy of Non-Small Cell Lung Cancer With Leptomeningeal Metastasis
title_short Unique Genomic Alterations of Cerebrospinal Fluid Cell-Free DNA Are Critical for Targeted Therapy of Non-Small Cell Lung Cancer With Leptomeningeal Metastasis
title_sort unique genomic alterations of cerebrospinal fluid cell-free dna are critical for targeted therapy of non-small cell lung cancer with leptomeningeal metastasis
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522975/
https://www.ncbi.nlm.nih.gov/pubmed/34671549
http://dx.doi.org/10.3389/fonc.2021.701171
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