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Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women

BACKGROUND: Human papilloma virus (HPV) is involved in the development of cancer of the cervix, mouth and throat, anus, penis, vulva, or vagina, but it has not been much considered as a cause of breast cancer. Recently, a number of investigations have linked breast cancer to viral infections. High-r...

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Autores principales: Gebregzabher, Endale, Seifu, Daniel, Tigneh, Wondemagegnhu, Bokretsion, Yonas, Bekele, Abebe, Abebe, Markos, Lillsunde-Larsson, Gabriella, Karlsson, Christina, Karlsson, Mats G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523267/
https://www.ncbi.nlm.nih.gov/pubmed/34671492
http://dx.doi.org/10.1155/2021/2140151
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author Gebregzabher, Endale
Seifu, Daniel
Tigneh, Wondemagegnhu
Bokretsion, Yonas
Bekele, Abebe
Abebe, Markos
Lillsunde-Larsson, Gabriella
Karlsson, Christina
Karlsson, Mats G.
author_facet Gebregzabher, Endale
Seifu, Daniel
Tigneh, Wondemagegnhu
Bokretsion, Yonas
Bekele, Abebe
Abebe, Markos
Lillsunde-Larsson, Gabriella
Karlsson, Christina
Karlsson, Mats G.
author_sort Gebregzabher, Endale
collection PubMed
description BACKGROUND: Human papilloma virus (HPV) is involved in the development of cancer of the cervix, mouth and throat, anus, penis, vulva, or vagina, but it has not been much considered as a cause of breast cancer. Recently, a number of investigations have linked breast cancer to viral infections. High-risk HPV types, predominantly HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, and 59, are established as carcinogens in humans. In this study we aimed to detect 19 high-risk and 9 low-risk HPVs from archived breast tumor tissue among Ethiopian women. METHODS: In this study, 75 breast cancer patients from Tikur Anbassa Specialized Hospital in Addis Ababa (Ethiopia) were included. HPV detection and genotyping were done using the novel Anyplex™ II HPV28 Detection Assay at the Orebro University Hospital, Sweden. The Anyplex™ II PCR System detects 19 high-risk HPV types (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 73, and 82) and 9 low-risk HPV types (6, 11, 40, 42, 43, 44, 54, 61, and 70). IHC for p16 was done using an automated system, the Dako Autostainer Link. RESULTS: Out of the 75 valid tests, two were found to be positive (2.7%) for HPV. One of the cases was positive for the high-risk HPV16 genotype while the other was positive both for the high-risk HPV39 and the low-risk HPV6. The cell cycle protein p16 was highly expressed in the case positive for the high-risk HPV16, but it was not expressed in the case positive for HPV39. CONCLUSION: The prevalence of HPV is low in Ethiopian breast cancer patients, but the role played by HPV in breast carcinogenesis among Ethiopian breast cancer patients cannot be commented based on these observations.
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spelling pubmed-85232672021-10-19 Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women Gebregzabher, Endale Seifu, Daniel Tigneh, Wondemagegnhu Bokretsion, Yonas Bekele, Abebe Abebe, Markos Lillsunde-Larsson, Gabriella Karlsson, Christina Karlsson, Mats G. Int J Breast Cancer Research Article BACKGROUND: Human papilloma virus (HPV) is involved in the development of cancer of the cervix, mouth and throat, anus, penis, vulva, or vagina, but it has not been much considered as a cause of breast cancer. Recently, a number of investigations have linked breast cancer to viral infections. High-risk HPV types, predominantly HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, and 59, are established as carcinogens in humans. In this study we aimed to detect 19 high-risk and 9 low-risk HPVs from archived breast tumor tissue among Ethiopian women. METHODS: In this study, 75 breast cancer patients from Tikur Anbassa Specialized Hospital in Addis Ababa (Ethiopia) were included. HPV detection and genotyping were done using the novel Anyplex™ II HPV28 Detection Assay at the Orebro University Hospital, Sweden. The Anyplex™ II PCR System detects 19 high-risk HPV types (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 73, and 82) and 9 low-risk HPV types (6, 11, 40, 42, 43, 44, 54, 61, and 70). IHC for p16 was done using an automated system, the Dako Autostainer Link. RESULTS: Out of the 75 valid tests, two were found to be positive (2.7%) for HPV. One of the cases was positive for the high-risk HPV16 genotype while the other was positive both for the high-risk HPV39 and the low-risk HPV6. The cell cycle protein p16 was highly expressed in the case positive for the high-risk HPV16, but it was not expressed in the case positive for HPV39. CONCLUSION: The prevalence of HPV is low in Ethiopian breast cancer patients, but the role played by HPV in breast carcinogenesis among Ethiopian breast cancer patients cannot be commented based on these observations. Hindawi 2021-10-11 /pmc/articles/PMC8523267/ /pubmed/34671492 http://dx.doi.org/10.1155/2021/2140151 Text en Copyright © 2021 Endale Gebregzabher et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gebregzabher, Endale
Seifu, Daniel
Tigneh, Wondemagegnhu
Bokretsion, Yonas
Bekele, Abebe
Abebe, Markos
Lillsunde-Larsson, Gabriella
Karlsson, Christina
Karlsson, Mats G.
Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women
title Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women
title_full Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women
title_fullStr Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women
title_full_unstemmed Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women
title_short Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women
title_sort detection of high- and low-risk hpv dna in archived breast carcinoma tissues from ethiopian women
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523267/
https://www.ncbi.nlm.nih.gov/pubmed/34671492
http://dx.doi.org/10.1155/2021/2140151
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