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Trilogy Development of Proopiomelanocortin Neurons From Embryonic to Adult Stages in the Mice Retina

Proopiomelanocortin-positive amacrine cells (POMC ACs) were first discovered in adult mouse retinas in 2010; however, the development of POMC-ACs has not been studied. We bred POMC-EGFP mice to label POMC-positive cells and investigated the development of POMC neurons from embryonic to adult stages....

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Autores principales: Zhang, Xuhong, Wang, Xiaoyu, Wang, Senjie, Peng, Wei, Ullah, Rahim, Fu, Junfen, Zhou, Yudong, Shen, Ye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523858/
https://www.ncbi.nlm.nih.gov/pubmed/34676208
http://dx.doi.org/10.3389/fcell.2021.718851
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author Zhang, Xuhong
Wang, Xiaoyu
Wang, Senjie
Peng, Wei
Ullah, Rahim
Fu, Junfen
Zhou, Yudong
Shen, Ye
author_facet Zhang, Xuhong
Wang, Xiaoyu
Wang, Senjie
Peng, Wei
Ullah, Rahim
Fu, Junfen
Zhou, Yudong
Shen, Ye
author_sort Zhang, Xuhong
collection PubMed
description Proopiomelanocortin-positive amacrine cells (POMC ACs) were first discovered in adult mouse retinas in 2010; however, the development of POMC-ACs has not been studied. We bred POMC-EGFP mice to label POMC-positive cells and investigated the development of POMC neurons from embryonic to adult stages. We found that POMC neuron development is mainly divided into three stages: the embryonic stage, the closed-eye stage, and the open-eye stage. Each stage has unique characteristics. In the embryonic stage, POMC neurons appeared in the retina at about E13. There was a cell number developmental peak at E15, followed by a steep decline at E16. POMC neurons showed a large soma and increased spine numbers at the closed-eye stage, and two dendritic sublaminas formed in the inner plexiform layer (IPL). The appearance and increased soma size and dendrite numbers did not occur continuously in space. We found that the soma number was asymmetric between the superior and inferior retinas according to the developmental topographic map. Density peaked in the superior retina, which existed persistently in the retinal ganglion cell layer (GCL), but disappeared from the inner nuclear layer (INL) at about P6. At the same time, the soma distribution in the INL was the most regular. At the open-eye stage, the development of POMC neurons was nearly stable only with only an increase in the IPL width, which increased the soma–dendrite distance.
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spelling pubmed-85238582021-10-20 Trilogy Development of Proopiomelanocortin Neurons From Embryonic to Adult Stages in the Mice Retina Zhang, Xuhong Wang, Xiaoyu Wang, Senjie Peng, Wei Ullah, Rahim Fu, Junfen Zhou, Yudong Shen, Ye Front Cell Dev Biol Cell and Developmental Biology Proopiomelanocortin-positive amacrine cells (POMC ACs) were first discovered in adult mouse retinas in 2010; however, the development of POMC-ACs has not been studied. We bred POMC-EGFP mice to label POMC-positive cells and investigated the development of POMC neurons from embryonic to adult stages. We found that POMC neuron development is mainly divided into three stages: the embryonic stage, the closed-eye stage, and the open-eye stage. Each stage has unique characteristics. In the embryonic stage, POMC neurons appeared in the retina at about E13. There was a cell number developmental peak at E15, followed by a steep decline at E16. POMC neurons showed a large soma and increased spine numbers at the closed-eye stage, and two dendritic sublaminas formed in the inner plexiform layer (IPL). The appearance and increased soma size and dendrite numbers did not occur continuously in space. We found that the soma number was asymmetric between the superior and inferior retinas according to the developmental topographic map. Density peaked in the superior retina, which existed persistently in the retinal ganglion cell layer (GCL), but disappeared from the inner nuclear layer (INL) at about P6. At the same time, the soma distribution in the INL was the most regular. At the open-eye stage, the development of POMC neurons was nearly stable only with only an increase in the IPL width, which increased the soma–dendrite distance. Frontiers Media S.A. 2021-10-05 /pmc/articles/PMC8523858/ /pubmed/34676208 http://dx.doi.org/10.3389/fcell.2021.718851 Text en Copyright © 2021 Zhang, Wang, Wang, Peng, Ullah, Fu, Zhou and Shen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Zhang, Xuhong
Wang, Xiaoyu
Wang, Senjie
Peng, Wei
Ullah, Rahim
Fu, Junfen
Zhou, Yudong
Shen, Ye
Trilogy Development of Proopiomelanocortin Neurons From Embryonic to Adult Stages in the Mice Retina
title Trilogy Development of Proopiomelanocortin Neurons From Embryonic to Adult Stages in the Mice Retina
title_full Trilogy Development of Proopiomelanocortin Neurons From Embryonic to Adult Stages in the Mice Retina
title_fullStr Trilogy Development of Proopiomelanocortin Neurons From Embryonic to Adult Stages in the Mice Retina
title_full_unstemmed Trilogy Development of Proopiomelanocortin Neurons From Embryonic to Adult Stages in the Mice Retina
title_short Trilogy Development of Proopiomelanocortin Neurons From Embryonic to Adult Stages in the Mice Retina
title_sort trilogy development of proopiomelanocortin neurons from embryonic to adult stages in the mice retina
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523858/
https://www.ncbi.nlm.nih.gov/pubmed/34676208
http://dx.doi.org/10.3389/fcell.2021.718851
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