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Compartment Niche Shapes the Assembly and Network of Cannabis sativa-Associated Microbiome

Interactions between plants and microbes may promote the growth of plants and regulate the production of secondary metabolites. Hemp (Cannabis sativa) is an annual herb and an important commercial crop. However, the assembly and network of hemp-associated microbiomes inhabiting in soil and plant com...

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Autores principales: Wei, Guangfei, Ning, Kang, Zhang, Guozhuang, Yu, Haibin, Yang, Shuming, Dai, Fei, Dong, Linlin, Chen, Shilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524047/
https://www.ncbi.nlm.nih.gov/pubmed/34675893
http://dx.doi.org/10.3389/fmicb.2021.714993
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author Wei, Guangfei
Ning, Kang
Zhang, Guozhuang
Yu, Haibin
Yang, Shuming
Dai, Fei
Dong, Linlin
Chen, Shilin
author_facet Wei, Guangfei
Ning, Kang
Zhang, Guozhuang
Yu, Haibin
Yang, Shuming
Dai, Fei
Dong, Linlin
Chen, Shilin
author_sort Wei, Guangfei
collection PubMed
description Interactions between plants and microbes may promote the growth of plants and regulate the production of secondary metabolites. Hemp (Cannabis sativa) is an annual herb and an important commercial crop. However, the assembly and network of hemp-associated microbiomes inhabiting in soil and plant compartments have not been comprehensively understood. This work investigated the assembly and network of bacterial and fungal communities living in soils (bulk and rhizosphere) and plant compartments (root, stem, leaf, and flower) of four hemp ecotypes cultivated in the same habitat. Microbiome assembly was predominantly shaped by compartment niche. Microbial alpha diversity was the highest in soil, continually decreased from root to flower. Core bacterial genera Pseudomonas, Bacillus, Rhizobium, Planococcus, and Sphingomonas were mostly enriched in aerial endosphere niches; Clitopilus, Plectosphaerella, and Mortierella were enriched in belowground endosphere. Microbial network complexity and connectivity decreased from root to flower. According to source tracking analysis, hemp microbiota primarily originated from soil and were subsequently filtered in different plant compartments. This work provides details on hemp-associated microbiome along the soil–plant continuum and a comprehensive understanding of the origin and transmission mode of endophytes in hemp.
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spelling pubmed-85240472021-10-20 Compartment Niche Shapes the Assembly and Network of Cannabis sativa-Associated Microbiome Wei, Guangfei Ning, Kang Zhang, Guozhuang Yu, Haibin Yang, Shuming Dai, Fei Dong, Linlin Chen, Shilin Front Microbiol Microbiology Interactions between plants and microbes may promote the growth of plants and regulate the production of secondary metabolites. Hemp (Cannabis sativa) is an annual herb and an important commercial crop. However, the assembly and network of hemp-associated microbiomes inhabiting in soil and plant compartments have not been comprehensively understood. This work investigated the assembly and network of bacterial and fungal communities living in soils (bulk and rhizosphere) and plant compartments (root, stem, leaf, and flower) of four hemp ecotypes cultivated in the same habitat. Microbiome assembly was predominantly shaped by compartment niche. Microbial alpha diversity was the highest in soil, continually decreased from root to flower. Core bacterial genera Pseudomonas, Bacillus, Rhizobium, Planococcus, and Sphingomonas were mostly enriched in aerial endosphere niches; Clitopilus, Plectosphaerella, and Mortierella were enriched in belowground endosphere. Microbial network complexity and connectivity decreased from root to flower. According to source tracking analysis, hemp microbiota primarily originated from soil and were subsequently filtered in different plant compartments. This work provides details on hemp-associated microbiome along the soil–plant continuum and a comprehensive understanding of the origin and transmission mode of endophytes in hemp. Frontiers Media S.A. 2021-10-05 /pmc/articles/PMC8524047/ /pubmed/34675893 http://dx.doi.org/10.3389/fmicb.2021.714993 Text en Copyright © 2021 Wei, Ning, Zhang, Yu, Yang, Dai, Dong and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wei, Guangfei
Ning, Kang
Zhang, Guozhuang
Yu, Haibin
Yang, Shuming
Dai, Fei
Dong, Linlin
Chen, Shilin
Compartment Niche Shapes the Assembly and Network of Cannabis sativa-Associated Microbiome
title Compartment Niche Shapes the Assembly and Network of Cannabis sativa-Associated Microbiome
title_full Compartment Niche Shapes the Assembly and Network of Cannabis sativa-Associated Microbiome
title_fullStr Compartment Niche Shapes the Assembly and Network of Cannabis sativa-Associated Microbiome
title_full_unstemmed Compartment Niche Shapes the Assembly and Network of Cannabis sativa-Associated Microbiome
title_short Compartment Niche Shapes the Assembly and Network of Cannabis sativa-Associated Microbiome
title_sort compartment niche shapes the assembly and network of cannabis sativa-associated microbiome
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524047/
https://www.ncbi.nlm.nih.gov/pubmed/34675893
http://dx.doi.org/10.3389/fmicb.2021.714993
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