An easy-to-use CRISPRi plasmid tool for inducible knockdown in E. coli

CRISPR-interference (CRISPRi) is a powerful genetic tool with wide application in bacteria. In this work a single plasmid system is presented which expresses deactivated Cas9 and a short guide RNA sequence from separate inducible promoters, enabling up to 40-fold repression of target genes with mini...

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Detalles Bibliográficos
Autor principal: Bradley, Robert W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524100/
https://www.ncbi.nlm.nih.gov/pubmed/34703773
http://dx.doi.org/10.1016/j.btre.2021.e00680

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524100/
https://www.ncbi.nlm.nih.gov/pubmed/34703773
http://dx.doi.org/10.1016/j.btre.2021.e00680

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