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An easy-to-use CRISPRi plasmid tool for inducible knockdown in E. coli
CRISPR-interference (CRISPRi) is a powerful genetic tool with wide application in bacteria. In this work a single plasmid system is presented which expresses deactivated Cas9 and a short guide RNA sequence from separate inducible promoters, enabling up to 40-fold repression of target genes with mini...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524100/ https://www.ncbi.nlm.nih.gov/pubmed/34703773 http://dx.doi.org/10.1016/j.btre.2021.e00680 |