Protocol to alter a protein’s phase separation capacity to control cell fate transitions

Phase separation of proteins regulates transcription. Here, we present a protocol to manipulate phase separation capacity of a protein. We use this protocol to disrupt phase separation by mutating residues at intrinsically disordered regions (IDRs). Further, we rescue the disabled phase separation b...

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Detalles Bibliográficos
Autores principales: Ma, Qian, Wang, Jia, Yu, Haopeng, Sun, Junyi, Sun, Jun, Chen, Jiahao, Yu, Yangyinhui, Fang, Peihang, Tian, Qi, Ding, Junjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524243/
https://www.ncbi.nlm.nih.gov/pubmed/34704075
http://dx.doi.org/10.1016/j.xpro.2021.100887

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524243/
https://www.ncbi.nlm.nih.gov/pubmed/34704075
http://dx.doi.org/10.1016/j.xpro.2021.100887

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