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The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR
Glycosylation plays a crucial role in the folding, structure, quality control and trafficking of glycoproteins. Here, we explored whether the glycosylation status of MHC class I (MHC-I) molecules impacts their affinity for the peptide editor, TAPBPR. We demonstrate that the interaction between TAPBP...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pergamon Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524320/ https://www.ncbi.nlm.nih.gov/pubmed/34543843 http://dx.doi.org/10.1016/j.molimm.2021.09.007 |
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author | Ilca, F. Tudor Boyle, Louise H. |
author_facet | Ilca, F. Tudor Boyle, Louise H. |
author_sort | Ilca, F. Tudor |
collection | PubMed |
description | Glycosylation plays a crucial role in the folding, structure, quality control and trafficking of glycoproteins. Here, we explored whether the glycosylation status of MHC class I (MHC-I) molecules impacts their affinity for the peptide editor, TAPBPR. We demonstrate that the interaction between TAPBPR and MHC-I is stronger when MHC-I lacks a glycan. Subsequently, TAPBPR can dissociate peptides, even those of high affinity, more easily from non-glycosylated MHC-I compared to their glycosylated counterparts. In addition, TAPBPR is more resistant to peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached. Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and –B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange. The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context. |
format | Online Article Text |
id | pubmed-8524320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Pergamon Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-85243202021-11-01 The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR Ilca, F. Tudor Boyle, Louise H. Mol Immunol Article Glycosylation plays a crucial role in the folding, structure, quality control and trafficking of glycoproteins. Here, we explored whether the glycosylation status of MHC class I (MHC-I) molecules impacts their affinity for the peptide editor, TAPBPR. We demonstrate that the interaction between TAPBPR and MHC-I is stronger when MHC-I lacks a glycan. Subsequently, TAPBPR can dissociate peptides, even those of high affinity, more easily from non-glycosylated MHC-I compared to their glycosylated counterparts. In addition, TAPBPR is more resistant to peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached. Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and –B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange. The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context. Pergamon Press 2021-11 /pmc/articles/PMC8524320/ /pubmed/34543843 http://dx.doi.org/10.1016/j.molimm.2021.09.007 Text en © 2021 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ilca, F. Tudor Boyle, Louise H. The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR |
title | The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR |
title_full | The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR |
title_fullStr | The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR |
title_full_unstemmed | The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR |
title_short | The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR |
title_sort | glycosylation status of mhc class i molecules impacts their interactions with tapbpr |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524320/ https://www.ncbi.nlm.nih.gov/pubmed/34543843 http://dx.doi.org/10.1016/j.molimm.2021.09.007 |
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