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Genome Engineering in Plant Using an Efficient CRISPR-xCas9 Toolset With an Expanded PAM Compatibility

The CRISPR-Cas9 system enables simple, rapid, and effective genome editing in many species. Nevertheless, the requirement of an NGG protospacer adjacent motif (PAM) for the widely used canonical Streptococcus pyogenes Cas9 (SpCas9) limits the potential target sites. The xCas9, an engineered SpCas9 v...

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Detalles Bibliográficos
Autores principales:	Zhang, Chengwei, Kang, Guiting, Liu, Xinxiang, Zhao, Si, Yuan, Shuang, Li, Lu, Yang, Yongxing, Wang, Feipeng, Zhang, Xiang, Yang, Jinxiao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Genome Editing
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525348/ https://www.ncbi.nlm.nih.gov/pubmed/34713242 http://dx.doi.org/10.3389/fgeed.2020.618385

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