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Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application
In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA designs, or Cas proteins, but can also be a platf...
Autores principales: | Yue, Jin-Jun, Yuan, Jin-Ling, Wu, Fu-Hui, Yuan, Yu-Hsuan, Cheng, Qiao-Wei, Hsu, Chen-Tran, Lin, Choun-Sea |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525356/ https://www.ncbi.nlm.nih.gov/pubmed/34713263 http://dx.doi.org/10.3389/fgeed.2021.717017 |
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