Baboon Envelope Pseudotyped “Nanoblades” Carrying Cas9/gRNA Complexes Allow Efficient Genome Editing in Human T, B, and CD34(+) Cells and Knock-in of AAV6-Encoded Donor DNA in CD34(+) Cells

Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells and living organisms. However, their delivery into human blood cells can be challenging. Here, we have utilized “nanoblades,” a new technology that delivers a genomic cleaving agent into cells. These are...

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Detalles Bibliográficos
Autores principales: Gutierrez-Guerrero, Alejandra, Abrey Recalde, Maria Jimena, Mangeot, Philippe E., Costa, Caroline, Bernadin, Ornellie, Périan, Séverine, Fusil, Floriane, Froment, Gisèle, Martinez-Turtos, Adriana, Krug, Adrien, Martin, Francisco, Benabdellah, Karim, Ricci, Emiliano P., Giovannozzi, Simone, Gijsbers, Rik, Ayuso, Eduard, Cosset, François-Loïc, Verhoeyen, Els
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Genome Editing
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525375/
https://www.ncbi.nlm.nih.gov/pubmed/34713246
http://dx.doi.org/10.3389/fgeed.2021.604371

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